Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Maurice Wilkins wikipedia , lookup
Agarose gel electrophoresis wikipedia , lookup
Non-coding DNA wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
SNP genotyping wikipedia , lookup
Gel electrophoresis of nucleic acids wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
DNA supercoil wikipedia , lookup
Cre-Lox recombination wikipedia , lookup
Molecular cloning wikipedia , lookup
FAQ: How many fragments of DNA can be assembled in one reaction? NEBuilder® HiFi DNA Assembly: Bridging dsDNA with a ssDNA Oligo Learn how NEBuilder® HiFi DNA Assembly bridges dsDNA with a ssDNA oligo. The number of DNA segments that can be assembled in one reaction is dependent on the length and sequence of the fragments. Gibson Assembly has been used to efficiently assemble up to twelve 0.4 kb inserts into a vector at one time. However, we recommend the assembly of five or fewer inserts into a vector in one reaction in order to produce a clone with the correct insert. A strategy involving sequential assembly can be used if all of the fragments cannot be assembled in a single reaction. Links to this resource Products: Gibson Assembly® Master Mix