Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download manual lens stretcher
Document related concepts
Transcript
Applica'on: eSEM images of the accommoda'on apparatus The BIONIKO Manual Lens Stretcher (MLS) is designed to mount and stretch the accommodation apparatus in ex-vivo eyes, to study structures including Applica'on: eSEM images of the accommoda'on apparatus Cliary processes. the ciliary body, the zonules and the lens. It provides researchers the means to handle these delicate tissue structures in order to simulate accommodation or perform morphological studies on the exposed structures. Its compact size allows the MLS to Stretched view of the accommodation apparatus, including the anterior hyaloid membrane. Zonular fiber attachment on lens capsule. be used within a larger optical or imaging instrument. As published by A. Bernal, J.M. Parel and F. Manns in Invest Ophthalmol Vis Sci. 2006 Nov;47(11):4708-13 www.bioniko.com Phone: (507) BIONIKO Email: [email protected] © Copyright 2014 BIONIKO BIONIKO MANUAL LENS STRETCHER (MLS) SHOES FEATURES There are 8 SHOES in a “SHOE-set”. The SHOES are the eye adapters of the MLS mechanism. They are radially arranged around the eye and glued to the sclera at the posterior chamber level below the limbus. SHOE-Sets come in “Sizes”, and are removable and inter-changeable. SHOE Size is specified with two numbers depending on eye biometry: “G”-”C” G - Average Globe Diameter (at equator) C - Average Corneal Diameter (at limbus) G ACCESSORIES • Exposure and handling of posterior chamber structures • Immersion chambers • Apertures • 4mm Ø Max stretch • Heated systems • Dissection stages • Anterior access to posterior structures, such as the anterior hyaloid membrane are possible • Illumination stages • Custom SHOES C Our Human standard SHOE size is: 24-12 (24mm globe diameter, 12mm corneal diameter). We “custom-fit” SHOES to match your research needs. SHOE-Sets are available in disposable plastic or in reusable aluminum. CASE CLICK FOR MLS ASSEMBLY VIDEO SET UP TOP PLATE 1. Prepare eye. Remove conjunctiva and dry scleral surface with a sponge. 2. Mount eye. With the stretcher in the unstretched position, add a drop of super-glue (cyanoacrylate) to the curved surface of the 8 shoes; align cornea with aperture and glue sclera to the shoes. Wait for a couple of minutes for glue to cure. 3. Dissect. Remove posterior pole and remove excess vitreous with a sponge and scissors. Dissect cornea and iris to expose the accommodation apparatus. 4. Segment. Cut sclera between segments, taking care not to cut through all the tissue. Only the scleral shell should be cut to allow stretching. The soft tissues beneath the sclera should be left as intact as possible. SHOES APPLICATIONS • Accommodation simulation BOTTOM PLATE • Ocular optics / IOL research • Morphology research; used with advanced microscopy techniques: SEM, eSEM, Confocal, AFM
