Download Human Chorionic Gonadotropin (hCG)

PA1181
US office:
Acris Antibodies, Inc.
San Diego, CA
UNITED STATES
Phone: +1-858-888-7900
Fax: +1-858-888-7904
[email protected]
Acris Antibodies GmbH
Schillerstr. 5
32052 Herford
GERMANY
Phone: +49-5221-34606-0
Fax: +49-5221-34606-11
[email protected]
Human Chorionic Gonadotropin (hCG)
Alternate names:
Choriogonadotropin subunit beta, CGB, CGB3, beta hCG, hCG-beta
Catalog No.:
PA1181
Quantity:
10 µg
Background:
Human chorionic gonadotropin (hCG) is a peptide hormone produced in pregnancy, that is
made by the embryo soon after conception and later by the syncytiotrophoblast (part of the
placenta). Its role is to prevent the disintegration of the corpus luteum of the ovary and
thereby maintain progesterone production that is critical for a pregnancy in humans. hCG
may have additional functions, for instance it is thought that it affects the immune
tolerance of the pregnancy. Early pregnancy testing generally is based on the detection or
measurement of hCG.
Uniprot ID:
P01233
Species:
Human
Source:
CHO cells Chinese Hamster Ovary Cells (CHO)
Format:
State: Sterile filtered, white lyophilized (freeze-dried) powder
Purity: >99% Purified by proprietary chromatographic techniques. Purity > 99.0 %,
determined by:
(a) Analysis by RP-HPLC, (b) Analysis by SDS-PAGE
Buffer System: Lyophilized from a concentrated (1 mg/ml) solution containing 110 mg
mannitol, 1.7 mg phosphoric acid, 0.4 mg L-Methionine and 0.2 mg poloxamer pH 7.
Reconstitution: Reconstitute in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can
then be further diluted to other aqueous solutions.
Description:
Human Chorionic Gonadotropin Recombinant produced in Chinese hamster ovary (CHO)
cells by recombinant DNA technology is a single glycoprotein chain consisting of two
non-covalently linked subunits - designated alpha and beta consisting of 92 and 145
amino acid residues (237 a.a. total, Mw: 36.7kDa), respectively, with carbohydrate
moieties linked to ASN-52 and ASN-78 (on alpha subunit) and ASN-13, ASN-30, SER-121,
SER-127, SER-132 and SER-138 (on beta subunit). The primary structure of the alpha chain
of r-hCG is identical to that of the alpha chain of hCG, FSH and LH.
The glycoform pattern of the alpha subunit of r-hCG is closely comparable to urinary
derived hCG (u-hCG), the differences mainly being due to the branching and sialylation
extent of the oligosaccharides. The beta chain has both O and N-glycosylation sites and its
structure and glycosylation pattern are also very similar to that of urinary hCG.
Biological Activity: The biological activity of choriogonadotropin alpha is determined by
using the seminal vesicle weight gain test in male rats described in the "Chorionic
Gonadotrophins" monograph of the European Pharmacopoeia. The in vivo biological
activity of choriogonadotropin alpha has been calibrated against the third international
reference preparation IS75/587 for chorionic gonadotropin.
For research and in vitro use only. Not for diagnostic or therapeutic work.
Material Safety Datasheets are available at www.acris-antibodies.com or on request.
AV/20081126
Antibody Hotline - Technical Questions - Antibody Location Service
Free Call: 0800-2274746 (Germany only) - www.acris-antibodies.com
1/2
PA1181: Human Chorionic Gonadotropin (hCG)
Specific Activity: - 26,000 IU/mg
Molecular weight: 37 kD
Storage:
Lyophilized hCG, although stable at room temperature for 3 weeks, should be stored
desiccated below -18°C. Upon reconstitution CG-beta should be stored at 2-8°C between
2-7 days and for future use below -20°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Shelf life: one year from despatch.
For research and in vitro use only. Not for diagnostic or therapeutic work.
Material Safety Datasheets are available at www.acris-antibodies.com or on request.
AV/20081126
Antibody Hotline - Technical Questions - Antibody Location Service
Free Call: 0800-2274746 (Germany only) - www.acris-antibodies.com
2/2