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Analytical and Bioanalytical Chemistry
Electronic Supplementary Material
Naked-eye detection as a universal approach to lower the limit of
detection of enzyme-linked immunoassays
Erin F. O'Connor, Sureyya Paterson, Roberto de la Rica
Fig. S1 Photograph of colored solutions in Figure 2a. 2 L was extracted from each
well and pipetted in a tube. The concentration in each sample is, from left to right, 0,
10-12, 10-11, 10-10, 10-9, 10-8, 10-7 and 10-6 g·mL-1. The limit of detection for naked eye
detection is 10-8 g·mL-1, which is the same as the limit of detection reported in Fig.
2a. This experiment demonstrates that the limit of detection is not affected by visual
artifacts, and therefore that the lower limit of detection in Fig. 3 is originated by
higher absorbance values.