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Photosynthesis Photosynthesis Process uses the sun’s energy to make glucose Carried out in green plants and some bacteria Purpose is to trap sun’s energy and store it in glucose (food for the plant) Photosynthesis occurs in the chloroplast photons SUN 6CO2 + 12H2O C6H12O6 + 6O2 + 6H2O carbon dioxide + water glucose + oxygen + water 2 6CO2 + 12H2O C6H12O6 + 6O2 + 6H2O carbon dioxide + water glucose + oxygen + water 3 Stomata (stoma) Pores in a plant’s cuticle through which water and gases are exchanged between the plant and the atmosphere. Oxygen (O2) & Water Carbon Dioxide (CO2) & Water Guard Cell Guard Cell Found on the underside of leaves Chloroplast Organelle where photosynthesis takes place. Stroma: gel filled space Outer Membrane Thylakoid: green disks Granum: stack Inner Membrane of thylakoids Inter Membrane Space Thylakoid stacks are connected together 6 Thylakoid Thylakoid Membrane Granum Thylakoid Space (Lumen) Grana make up the inner membrane 7 Why are plants green? Electromagnetic Spectrum Pigments Pigments are light-absorbing compounds. Pigments appear colored because they absorb light of certain wavelengths and reflect that of others. Chlorophyll a and b are the primary pigments in green plants that absorbs red and blue/violet light and reflects green light. Structure of Chlorophyll Accessory Pigments Chloroplasts also contain other pigments called accessory pigments that capture other wavelengths to absorb more energy. Carotenoids-yellow/orange Anthocyanin-red/purple Photosynthesis Reactions CO2 Dark Reactions ATP NADPH H2O + CO2 Sugars Light Reactions O2 14 Light and Dark Reactions Photosynthesis Steps 1. Light reaction (Light-dependent reaction) Chlorophyll traps sunlight and makes electrons and ATP to run the dark reaction. 2. Dark reaction (Light-independent reaction) Calvin Cycle uses ATP and electrons from the light reaction and carbon dioxide from the air to make glucose. ATP All organisms require energy for: Active transport Cell division Movement Production of proteins 17 NADPH NADP+ is an electron acceptor NADP+ + 2e- + 2 H+ NADPH + H+ LIGHT REACTION Requires light Pigments absorb photons of light and excite electrons Occurs in two photosystems in the thylakoid membranes Photosystem: a unit of several hundred chlorophyll molecules and associated acceptor molecules Light Reaction Summary Input: light energy H2O Output: ATP NADPH O2 20 Dark Reaction (Calvin Cycle) American scientist Melvin Calvin Occurs in the stroma. Uses ATP and NADPH from light reactions. Uses CO2 to produce glucose (C6H12O6). To produce 1 glucose: it takes 6 turns of the cycle and uses 18 ATP and 12 NADPH. Dark Reactions Summary Input: ATP NADPH CO2 Output: ADP NADP+ H2O Sugars (glucose) 22 Rate of Photosynthesis The rate at which a plant can carry photosynthesis is affected by its environment. 1. Temperature – enzymes directly impacted 2. Light intensity – no light = no photosynthesis 3. Water - required 4. [CO2]- Calvin (Dark) cycle requirements The End!