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LUmo
Microplate Chemiluminescence Immunoassay( CLIA) System
A
..
utobio
autobio diagnostics
an autobio group company | www.autobio-diagnostics.com
• 13485
ISO 9001
C E R T I F I E D IVD C
LUmo
Autobio LUmo is a state of the art microplate luminometer,
designed to meet the demanding requirements of today’s
microplate based, glow luminescence applications.
Product highlights
High sensitivity 1 x 10-23 mole HRP
… allows detection of lower analyte concentrations, earlier
diagnosis of diseases and efficient tracing of treatments.
Large dynamic range 0 to 1.6 x 109 RLU
… significantly increases assay linearity and enables reliable
interpretation of very low and very high analyte concentrations
in one test run without additional sample dilution.
Very low crosstalk
LUmo incorporates a sophisticated, adaptive positioning system,
keeping the detector as close as possible above the sample to
eliminate instrument related crosstalk and make optimum use
of LUmo‘s high sensitivity and dynamic.
Speed
It takes typically less than 40 seconds to read a 96-well plate
at 0.1 sec integration time. With fast Luminol-based reaction
systems, high reading speed greatly improves assay precision
across a plate.
Specifications LUmo
Detection system
Photomultiplier module
Detection mode
Glow luminescence
Spectral Range
300 - 650nm
Dynamic / Indication
Range
0 - 1.600.000.000 RLU
Measurement unit
RLU (Relative Light Units)
Sensitivity at 545nm
1 x 10-23 mole HRP
Repeatability
< 2%
Crosstalk
< 1 x 10-7 (typical)
Measurement time
typically <40 seconds at 0.1 sec.
integration time for a 96-well
plate
Integration time
0.1 sec to 10 sec in increments of
0.1sec
Plate types
96-well and 48-well (4x12) microplates, max. height 15.2 mm
Shaking
3 modes
Dimensions
33.5cm x 20.5cm x 47.0 cm (w
x h x l)
Weight
~13.0kg net
Power supply
external
voltage range
100-240VAC ±10%, 47-63Hz
Data connections
Serial Interface RS232 (9-pin),
USB 2.0
Environmental conditions
Operating
Temperature: 10°C to 40°C
Humidity: 15%-75% RH noncondensing
Storage
Temperature: -10°C to 50°C
Humidity: <95% RH non-condensing
Scope of supply
Instrument, power supply, power
cable, dustcover, serial cable,
USB cable, AUTOsoft - Autobio
Control and Evaluation Software
(Windows®) and user manual
on CD
AUTOsoft
AUTOsoft control and evaluation software
Autobio considers software used to control the instruments and
perform the required evaluation as integral part of an efficient
system, focussed on processing the variety of microplate based
applications. AUTOsoft – Autobios powerful Windows® control
and evaluation software – is designed to perform the vast majority of routine and scientific applications.
Internal quality control function
… used to monitor and statistically analyze controls over time
and test runs.
AUTOsoft features and highlights
Plate Layouts
… can be defined and saved independent from the test definition using convenient positioning, filling and replicate functions.
Quantitative evaluation:
Curve fits modes: linear regression, point to point, cubic spline
and 4 parameter fit
Axis scaling: linear, logarithmic, logit-log axis scaling and extrapolation option
Multitest and Kindling test
Up to 12 different assays per sample (multitest) or up to 5
subsequently drawn samples of one patient (kindling test) can
be processed on one plate.
Sample IDs and patient data
can either be entered manually, generated automatically, or
imported from a file. The corresponding results are displayed
together with the sample data or printed in a sample report.
Directory
…is used to manage commonly used data and simplify routine
data entry.
Standard curves can be stored and used for subsequent test
runs as measured or adjusted by single or dual point calibration.
OEM customization and multilingual user interface
The user interface of AUTOsoft can easily be customized to
match the requirements of OEM customers regarding graphics
and language.
CLIA Diagnostic System
Principle of CLIA
Chemiluminescence Immunoassay (CLIA) using Microplate
luminometers provides a sensitive, high throughput, and
economical way to quantitatively measure antigen in the
samples.
Luminescence enhancer
Application of HRP-luminol system, with the addition of
luminescence enhancer, the substrate solution provides a
more reliable and sensitive value.analytical procedures whose
performance far exceeds that of immunoassays based on the
use of radioactive labels.
10000
with enhancer
without enhancer
9000
RLU
8000
7000
6000
0
CLIA platform introduction
CLIA is the most widely used technical platform
Chemiluminescence immunoassay is now established as
one of the best alternatives to conventional ELISA for the
low concentrations of analytes in complex samples. During
the last two decades the technology has evolved into
analytical procedures whose performance far exceeds that of
immunoassays based on the use of radioactive labels.
CLIA
ELISA
70s
80s
90s
15
Minute
20
25
2000
Comparison of CLIA and ELISA
2020
Not required long incubation time.
No addition of stopping reagent (colorimetric method is
required).
More economical compared to conventional colorimetric
method (Ex: Enzyme-linked immunosorbent assays, ELISA)
More sensitive compare to the colorimetric method
30
Luminescence enhancer produces a long-lasting light
emission instead of a short peak kinetics
The advantage of Autobio CLIA
The advantage of CLIA is the increased sensitivity & dynamic
range, which allows detection of lower analytes concentrations
& earlier diagnosis of disease. The luminol in the tracer reagent
will enhance the signal strength a long-lasting glow reaction
that is superior to other substrates. This results in an assay up to
1.6x109more sensitive than ELISA.
glow
flash
65000
50000
RLU
60s
10
80000
RIA
20 th
5
35000
20000
0
5
10
15
Minute
20
25
30
35
High sensitivity and reliability of glow reaction will bring
better result in the CLIA test.
CLIA Kits
Test Menu
Items
Packaging
Cat.no
HBsAg
96T
CL0310-2
Anti-HBs
96T
CL0311-2
HBeAg
96T
CL0312-2
Anti-HBe
96T
CL0313-2
Anti-HBc
96T
CL0314-2
AFP
96T
CL0203-2
CEA
96T
CL0205-2
PSA
96T
CL0206-2
f-PSA
96T
CL0208-2
HCG
96T
CL0106-2
CA125
96T
CL0209-2
CA153
96T
CL0210-2
CA199
96T
CL0211-2
CA50
96T
CL0207-2
SF ( ferritin)
96T
CL0212-2
T3
96T
CL1001-2
T4
96T
CL1002-2
TSH
96T
CL1003-2
FT3
96T
CL1004-2
FT4
96T
CL1005-2
Anti-TG
96T
CL1006-2
Anti-TPO
96T
CL1007-2
LH (Luteinizing hormone)
96T
CL1101-2
FSH( Follicle Stimulating Hormone)
96T
CL1102-2
PRL (Prolactin)
96T
CL1103-2
P (Progesterone)
96T
CL1104-2
T (Testosterone)
96T
CL1105-2
96T
CL1106-2
Hepatitis
Autobio CLIA products
Our CLIA kits employ glow-based chemiluminescent reactions.
Our series of the kits covers Thyroid function markers, Gonadal
hormones, Tumor markers, Diabetes marker, infectious disease
and other markers. They can be used to replace ELISA that has
been widely used in many research and diagnostic applications.
Tumor Markers
Thyroid
Fertility
Hypertension
Adrenocorticotropic Hormone
(ACTH)
96T
CLJ0202
Aldosterone (ALD)
96T
CLJ0102
E2 (Estradiol)
Cortisol (Cor)
96T
CLJ0302
Diabetes
Renin
96T
CLJ0402
Insulin (INS)
96T
CL1201-2
Angiotensin II (A II)
96T
CLJ0502
C-peptide (C-P)
96T
CL1202-2
Autobio Diagnostics Co., Ltd.
offers more than 200 in vitro diagnostic products including CLIA(microplate based CLIA and magnetic
particle based CLIA), ELISA, POCT (Point of Care Test), Microbiology . As an ISO 9001, EN ISO13485 and
GMP certified manufacturer, Autobio supplies high quality products through its well established sales
network and is reknown as a reliable OEM partner. For details please visit www.autobio-diagnostics.com
Autobio Diagnostics Co., Ltd. | No. 87 Jingbei Yi Road | National Eco & Tec Development Area | Zhengzhou City | China 450016
T: +86-371-6798-5313 | F: +86-371-6798-5804 | E: [email protected] | W: www.autobio-diagnostics.com
flyer Microplate CLIA 20140715e