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CELL PHYSIOLOGY CELLS ARE THE BASIC UNIT OF LIFE CELL ORGANELLES Mitochondrion Nucleus CELL ORGANELLES Cell Membrane Nucleus Endoplasmic reticulum Golgi complex Mitochondria Lysosomes Microfilaments and microtubules Vesicles THE NUCLEUS Site of DNA (In chromosomes) Enclosed by a membrane DNA REPLICATION The double helix “unzips” New bases pair up with the old ones The molecule replicates itself Two identical copies each with one old strand and one new RNA TRANSCRIPTION Messenger RNA “copies” from DNA m-RNA caries the message to the rough ER ENDOPLASMIC RETICULUM Fluid filled membrane system Rough ER: Protein Synthesis Smooth ER: Synthesis of Lipids Rough ER : Site of Protein Synthesis Rough ER contains ribosomes M-RNA attaches to ribosome A triplet of bases is a Codon Transfer RNA has an anticodon at one end and the appropriate amino acid at the other As the code is read, amino acids are assembled into a protein ER -> Golgi -> Secretion Exocytosis Vesicles fuse with the membrane They open to the outside and discharge their contents MITOCHONDRIA Extract Energy from Food Fuels Energy is stored in ATP Aerobic Metabolism Anaerobic Metabolism Sugar can be burned without oxygen - anaerobically Far more energy released from burning sugar aerobically Glycolysis is anaerobic-carried out in cytosol Glucose ----> 3 Carbon fragments plus 2 ATP Aerobic Metabolism Pyruvic Acid (3 C fragment) enters Mitochondria Combines with Coenzyme A loosing a CO2 and becoming Acetyl Coenzyme A (2 C fragment) This fragment enters a cyclic reaction scheme, the Citric Acid Cycle Ultimately, 34 more ATP’s are produced THE CYTOSKELETON Microtubules Microfilaments Intermediate Filaments Microtubular Lattice