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Microscopes and Microscopy Quiz
Name:_______________
Class:_________
Date:_______________
1. Base your answer to the following question on
the diagram of a microscope below and on your knowledge of
biology.
6. Base your answer to the following question on The diagram
below shows how a coverslip should be lowered onto some
single-celled organisms during the preparation of a wet mount.
Why is this a preferred procedure?
1)
2)
3)
4)
The coverslip will prevent the slide from breaking.
The organisms will be more evenly distributed.
The possibility of breaking the coverslip is reduced.
The possibility of trapping air bubbles is reduced.
7. Base your answer to the following question on A compound
light microscope is represented in the diagram below.
State two ways the image seen through the microscope differs
from the actual specimen being observed.
2. A student prepared a slide of pollen grains from a flower. First
the pollen was viewed through the low-power objective lens
and then, without moving the slide, viewed through the highpower objective lens of a compound light microscope.
Which statement best describes the relative number and
appearance of the pollen grains observed using these two
objectives?
1) low power: 25 small pollen grains; high power: 100 large
pollen grains
2) low power: 100 small pollen grains; high power: 25 large
pollen grains
3) low power: 25 large pollen grains; high power: 100 small
pollen grains
4) low power: 100 large pollen grains; high power: 25 small
pollen grains
3. While viewing a specimen under high power of a compound
light microscope, a student noticed that the specimen was out
of focus. Which part of the microscope should the student turn
to obtain a clearer image under high power?
1) eyepiece
3) fine adjustment
2) coarse adjustment
4) nosepiece
4. After switching from the high-power to the low-power objective
lens of a compound light microscope, the area of the
low-power field will appear
1) larger and brighter
3) larger and darker
Which microscope part is correctly paired with its function?
1) A – magnifies the image of the specimen
2) B – used for focusing only when the high-power objective
is used
3) C – provides the field of view with the largest diameter
4) D – holds the specimen on the stage
8. The total magnification of an image formed by a compound
light microscope is a result of the combined magnifications of
the
2) smaller and brighter
4) smaller and darker
5. What is the highest magnification that can be obtained with a
microscope that has a 10× eyepiece with 10× and 43×
objectives?
1) 43×
2) 100×
3) 430×
4) 4300×
Page 1
1)
2)
3)
4)
eyepiece and diaphragm
objective and mirror
eyepiece and objective
low-power objective and high-power objective
9. Base your answer to the following question on Which sequence contains the correct order of steps for a student to
follow to observe the nucleus of protozoa in a stained wet mount, using a compound light microscope?
1) 1
2) 2
3) 3
4) 4
10. Base your answer to the following question on the diagrams
below and on your knowledge of biology.
13. Which paragraph describes the correct procedure for
preparing a stained wet mount of onion epidermis?
1) Place a piece of onion epidermis on a slide. Add two
drops of water and one drop of stain. Cover the slice by
dropping a cover slip directly on top of it. Press the
coverslip to force air bubbles out. Add one drop of water
to one edge of the coverslip, and add one drop of stain
to the opposite edge.
2) Add one drop of stain to a piece of onion epidermis.
Using forceps, place the epidermis on a slide. Blot the
epidermis with a piece of paper towel to remove the
excess stain. Drop a coverslip onto the specimen.
3) Place a piece of onion epidermis on a slide. Add one drop
One similarity between all the microscopes shown is that they
1)
2)
3)
4)
lack a diaphragm and coarse adjustment
have three oculars
depend on electrons passing through the specimen
of water. Put one edge of a coverslip in the water drop,
then slowly lower the opposite edge to the water. Put one
drop of stain at one edge of the coverslip. Put a piece of
paper towel at the opposite side of the coverslip. Allow
the towel to absorb some water so that the stain will move
under the coverslip.
have two objective lenses
11. Base your answer to the following question on the diagram
below of a microscope and on your knowledge of biology.
4) Add one drop of stain to a slide. Place a piece of onion
epidermis on top of the stain. Use a piece of paper towel
to absorb the stain. Drop a coverslip on the epidermis to
flatten it out. Lift the coverslip and add a drop of water to
the epidermis. Replace the coverslip.
14. A slide of human blood cells was observed in focus under the
low-power objective of a compound light microscope that had
clean lenses. When the microscope was switched to high
power, the image was dark and fuzzy. Which parts of the
microscope should be used to correct this situation?
1)
2)
3)
4)
The highest possible magnification that can be obtained when
using this microscope is
1) 40×
2) 100×
3) 400×
4) 4,000×
12. A coverslip should be used for preparing a
1)
2)
3)
4)
frog for dissection
solution of iodine for food testing
wet mount of elodea (a simple plant)
test to determine the pH of a solution
Page 2
nosepiece and coarse adjustment
diaphragm and ocular
objective and fine adjustment
diaphragm and fine adjustment
15. Base your answer to the following question on the diagram below and on your knowledge of biology.
Which laboratory technique is illustrated in the diagram?
1)
2)
3)
4)
testing a specimen for amino acids
determining the pH of a specimen
measuring the photosynthetic rate in a specimen
preparing a wet mount of a specimen
Page 3
Answer Key
Microscopes and Microscopy Quiz
1.
— enlarged — upside
down — backward —
more detail
2.
2
3.
3
4.
1
5.
3
6.
4
7.
1
8.
3
9.
1
10.
4
11.
3
12.
3
13.
3
14.
4
15.
4
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