Download Interesting facts: • There are several different types of cell lines that

NPTEL – Biotechnology – Cell Biology
Interesting facts:
• There are several different types of cell lines that can be finite or continuous, prepared
from normal tissue as well as neuroplastic tissues.
• Cell bank system is generally used for maintenance of cell line with constant supply of
starting material as well as it perform cell lines characterization and detection of cell
line cross contamination.
• In drug discovery, stably transfected cell lines are generally used.
Questions:
1. What are the basic types of cell lines and which type of tissue involve in formation of
cell line?
2. What are the conditions required for cell line growth?
3. What are the criteria for choosing cell lines?
4. Which type of precaution one should take during cell line maintenance?
5. Give some example of human cell lines and what are the characteristic of those cell
lines?
References:
1. Fresheny, I. “Culture of animal cell – A manual of basic technique and
specialized application” by; chaptor-2: Biology of cultured cells; page-23
2. http://www.biology online.org/dictionary/Cell_line
3. http://atcc.custhelp.com/app/answers/detail/a_id/355/~/definition-of-%22cellline%22
4. http://www.biology-online.org/dictionary/Stock_culture
5. http://bio.lonza.com/uploads/tx_mwaxmarketingmaterial/Lonza_TechREF_Gener
ation_of_Stable_Cell_Lines_low_res.pdf
Joint initiative of IITs and IISc – Funded by MHRD
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