Download Recombinant Human GM-CSF

PRODUCT DATA SHEET
Recombinant Human GM-CSF
Catalog Number: 90025
Description: GM-CSF is a cytokine that controls the production, differentiation, and function of granulocytes and
macrophages. The active form of the protein is found extracellularly as a homodimer. The GM-CSF gene has been localized to
a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5qsyndrome and acute myelogenous leukemia.
PRODUCT SPECIFICATIONS
Product Details: Purified, full-length human recombinant
GM-CSF protein (amino acids 18-144, 127 a.a.) produced in
human cells. (Accession NP_000749.2; UniProt P04141)
Source: Human cell line
Predicted Molecular Weight: 14.5 kDa
Tag: StrepII at N
Cell Culture Medium: Chemically-defined, serum- and
animal-product-free culture medium
Purity: >95% pure by SDS-PAGE
Endotoxin: <0.1EU per g protein by LAL method
Biological Activity: ED50 is 0.1-0.25 ng/ml as determined
by a TF-1 cell proliferation assay
Formulation: Lyophilized from 0.2µm filtered solution in
0.3X PBS with 10% Trehalose (carrier-free).
Reconstitution: Centrifuge the vial prior to opening.
Reconstitute in PBS to a concentration of 0.1 mg/ml.
Stability & Storage: 12 months at -20°C as supplied; 1
month at 4°C after reconstitution. Avoid freeze/thaw
cycles.
Sequence: APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETS
CATQIITFESFKENLKDFLLVIPFDCWEPVQE
SDS-PAGE of 2 g purified recombinant GM-CSF.
The bioactivity of the purified recombinant GM-CSF was
determined by a TF-1 cell proliferation assay.
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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