Download Slide

From: Basement Membrane-Dependent Modification of Phenotype and Gene Expression in Human Retinal Pigment
Epithelial ARPE-19 Cells
Invest. Ophthalmol. Vis. Sci.. 2004;45(8):2786-2794. doi:10.1167/iovs.03-0943
Figure Legend:
ARPE-19 cells show a quantitative increase in rod outer segment phagocytosis after culture on PLC membranes.
ARPE-19 cells were grown to confluence on either Transwell polyester filters or PLC membranes and maintained at
confluence for a further 6 weeks. Cells were cultured in the presence of Alexa Fluor 555-labeled rod outer segments
(ROS) for 3 hours, fixed, and the ROS internalization monitored using laser scanning confocal microscopy. Sections
through the middle of the cells were recorded and density maps of the fluorescence established by integrative density
analysis. (A) Shown are pseudocolored density maps of representative images. Phagocytosed ROS appear in green,
veryofsmall
and faint,
possibly pinocytic
in red,
large,invery
bright
representing
aggregated
ROS
in reserved.
Date
download:
6/11/2017
Thevesicles
Association
for and
Research
Vision
and objects
Ophthalmology
Copyright
© 2017. All
rights
white. (B) Quantitative assessment of ROS phagocytosis (green areas in panel A) from ten distinct fields of view.