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Human Skin Tissue Equivalent: A Model for Human Skin Studies
Verhoeff's Elastic Stain: collagen in the dermis
is stained red, cell nuclei are stained black and
the epidermis is stained dark brown.
Keratinocytes will form a functional
stratum corneum
Epidermal
Layer
Keratinocytes in basal layer
Dermal
Layer
Dermal fibroblasts, which
produce:
Collagen, elastin, MMP-1,
and hyaluronic acid
Human Skin Tissue Equivalent: A Model for Human Skin Studies
Alcian Blue (pH 2.5) Stain: Hyaluronic acid in
the dermis is stained blue. Nuclear fast red
was used to counterstain the epidermis
Epidermal
Layer
Hyaluronic Acid:
Dermal
Layer
1.
Highly charged glycosaminoglycan found in
the extracellular environment.
2.
Attracts water, forming a gel.
3.
Cosmetic function: provides volume to skin.
4.
Loss of HA with aging (skin atrophy)
5.
Materials which can increase HA production
can have an anti-aging effect by providing
volume to aged skin (anti-wrinkling effect)