Download SMART Team

The Battle Within:
The Influenza Virus and the Keepers
of the Body
Tyler Ebert,Trevor Kraeger,
Tyler Stokka, Lucas Dahlke, Kyle Schwan,
Jordan Kappel
MSOE CBM Dr. Colton reference
DCE SMART team
World Pandemic
In recent times, the H1N1 Influenza
Virus has infected millions of people
world wide.
SMART Team
 Students Modeling A Research Topic
 Objective: To learn about the world of
proteins and viruses through
modeling and research.
Milwaukee School of
Engineering
Our Project
 This Years Project: The NS1 Protein, a
key component of the H1N1 virus,
and the Interferon Protein, the red
alert agent of the bodies.
Virus on the War Path
Every moment of every day, viruses
are attempting to infiltrate our
bodies.
Viral Anatomy
Enemy At the Gate
Behind Enemy Lines
A New Hope:Immunity
 The biological defenses our bodies
use to combat a wide range of
pathogens.
Native Immunity
 Native Immunity is your first line
defense against invader, does not
discriminate one foreign substance
from another. It has a policy of kill
first, ask questions later.
Inflammation
 A non-specific, physiologic reaction of
vascular tissue to injury and microbial
invasion. Recognized by the presence
of redness, swelling, heat, and pain.
 Without inflammation and repair,
infections would go unchecked and
wounds would never heal.
Image of Inflammation
Acquired Immunity
 Developed immune response against
certain immunogens after exposure to
identical or similar viruses.
Before
After
Interferons: The Call to Action
When a virus has infiltrated the cell a protein called TRIM 25 is
responsible for identifying their presence. TRIM 25 then hooks up
with RIG 1 who upon receiving the news begins to produce
Interferons.
Interferons then travel to every nearby cell alerting them of the
imminent danger and allowing them to prepare to defend their
homelands.
Visualization of the Interferons
The Best Laid Plans Go Astray
 The H1N1 gains its potency among
human because at key component
the NS1 Protein.
 The NS1 has a unique structure that
allows it insert itself into the TRIM 25
protein. Without being able to
connect to RIG 1 no interferons are
release. Therefore, the enemy goes
undetected.
Live Demonstration
X-Ray crystallography
X Ray
Crystallography
data obtained
from the Protein
Data Bank
Notice the X,Y, Z
coordinates are given for
each atom from the Xray
Data
Our Model: NS1
Our Model: Interferons
Bibliography: One Site To Show
Them All, One Site To Find Them.
www.medicineworld.org/
www.msoe.com
www.wikipedia.org
www.howstuffworks.com
www.youtube.com
www.pdb.org
www.facebook.com/lordoftheringsfanclub
One Site to Hold Them All, And
In Its Brightness Bind Them.
GOOGLE SCHOLAR