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Interface NTR CTR a Buried area (Å2)a, b 3110 2950 ΔGint ( kcal/mol)a, c -32.1 -23.8 ΔGdiss, (kcal/mol)a, d 9.3 2.0 The value were calculated using the program Protein Interfaces, Surface, and Assemblies (PISA). b Buried area indicates the solvent-accessible surface area of monomeric units buried upon assembly formation c ΔGint indicates the solvation free energy gain upon formation of the assembly. Negative values of ΔGint mean that subunits favor to from complex. d ΔGdiss indicates the free energy of assembly dissociation. Positive values of ΔGdiss indicate that an external driving force should be applied in order to dissociate the assembly, therefore assemblies with ΔGdiss over 0 are thermodynamically stable.