Download Biotechnology Pre-Test

Name: _______________________________
Per. ___ Date: ________
Biotechnology Pre-Test
Write the word or words that best describe each statement next to each question below. This is not for a grade, but
do attempt each question even if you are not sure.
1. In the gel below, which lane appears to have no restriction sites for EcoRI, the enzyme mixed with
each sample of DNA? Explain your answer.
A B
C
D
E
F
2. Which band(s) in the gel have the shortest pieces of DNA?
How do you know?
3. All of the genetic material from an organism is called a(an)
__________________.
4. What is the object used to aliquot very small amounts of
liquid? _______________________
5. What is the technique that separates DNA according to its
net charge and size in an agarose gel?
___________________________________
6. Name the process of amplifying large amounts of DNA for
further study or manipulation. (write full name) ______________________________
7. The separation of a two-stranded DNA molecule by heating is called ___________________.
8. Bonding of complementary single-stranded primers during the second stage of PCR is called
__________________________.
9. Enzymes isolated from bacteria that cut DNA at specific base sequences are called
________________________________.
10. Circular DNA found in bacteria is called a(n) _________________________.
11. Select the appropriate micropipette and show what the dial should read to measure each of the
following amounts of liquid.
Write A, B, C, or D on the line.
12. How many micrometers are in one millimeter?
13. What is the symbol for micrometer?
14. What part of the DNA is removed from the pre-mRNA or spliced before the final transcript is
created?
15. When loading a centrifuge, what is the #1 rule? Why is this rule important?
16. What volume can each pipette below accurately hold? What volume is each of the following
micropipette's holding?
A
A ______
B
B ______
C
C ______
Teachers: appoint the students who get both #11 & 16 correct to be pipette "experts" in the event
that many students need your help verifying their micropipette settings during the lab.
Answers
1. lane B – there is only one band so it wasn’t cut
2. the DNA in lane F (perhaps A and C) - they traveled the farthest from the wells
3. genome
4. micropipette
5. gel electrophoresis
6. polymerase chain reaction
7. denaturation
8. annealing
9. restriction enzymes
10. plasmid
11. B - 025, D - 070 (these two can be in any order), C - 030, A - 150
12. 1000
13. µ
14. intron
15. BALANCE = make sure you space the tubes equally, otherwise the motor can burn out
16. A: range 2-20 µl, volume 5.2 µl
B: range 20-200 µl, volume 70 µl
C: range 100-1000 µl, volume 150 µl