Download Student Learning Outcomes

Annexure ‘CD – 01’
FORMAT FOR COURSE CURRICULUM
Course Title: Bacteriology
L
T
P/S
SW/
FW
2
0
4
2
Course Code: MBT601
TOTAL
CREDIT
UNITS
05
Credit Units: 3+2
Course Objectives:
 The objective of the course is to illustrate on the basic principles of Bacteriology, metabolism, life cycle, genetics, and importance of microbes in dayto-day life.
 The course also aims to demonstrate fundamental knowledge of metabolic reactions in Bacterial cell, their growth and development and relevance to
applied microbiology.
Pre-requisites: Basic knowledge of Life Sciences
Student Learning Outcomes:
 The students will define about the fundamentals of microbiology and current research in bacteriology.
 Students will describe the fundamental understanding of the biology of microorganisms, the Genetic, metabolic, and systematic diversity of
microbial life and the diverse roles microorganism play in the environment, including the multitude of applications of microbes in biotechnology,
the food industry, agriculture, and medicine.
 Students can demonstrate an understanding, and ability to use, the scientific methods including observation, hypotheses testing, data collection, and
analysis.
 Critical analysis and evaluation of published research in the field of bacteriology.
Course Contents/Syllabus:
Module I
Morphology and ultra structure of bacteria - morphological types - cell walls of archaebacteria - gram negative - gram
positive eubacteria - eukaryotes, L-forms - cell wall synthesis, antigenic properties - capsule - types, composition and
function, cell membranes - structure - composition - properties. Structure function of flagella- cilia – pili -gas vesicles chromosomes, carboxysomes - magnetosomes and phycobolisomes - nucleoid - cell division - spores.
Module II
Reserve food materials- polyhydroxybutyrate - polyphosphate granules - oil droplets cyanophycin granules and sulphur
inclusions.
Module III
Cultivation of bacteria - aerobic - anaerobic - shaker - still - nutritional types - culture media used - growth curve generation time - growth kinetics - asynchronous - synchronous - batch - continuous culture - measurement of growth
and factors affecting growth, control of bacteria - physical and chemical agents - preservation methods.
Module IV
Classification of microorganisms - Introduction - Haeckel's three kingdom concept - Whittaker's five kingdom concept three domain concept of Carl Woese, Basis of microbial classification, Classification and salient features of bacteria
according to the Bergey's manual of determinative bacteriology, cyanobacteria, prochlorons and cyanelles.
Module V
Early discovery of pathogenic microorganisms; development of bacteriology as scientific discipline; contributions made
by eminent scientists. Classification of medically important microorganisms; Normal microbial flora of human body;
role of the resident flora and the human host. Sporing and non sporing anaerobes, organisms belonging to
Enterobacteriaceae, Non-fermenting Gram negative bacilli.
Lab/ Practical details, if applicable:
•
•
•
•
•
•
•
Introduction to types of media
Isolation of microbes from soil using serial dilution technique
Isolation of microflora using soil plating technique
Staining and microscopic observation
Measurements using micrometer
Growth curve preparation for fungi
Effect of fungicides on bacteria and fungi
Weightage (%)
30
10
20
20
20
Pedagogy:
(a) Lecture Plan/Session Plan : Lectures, Writing on Board, Power point
(b) Lab Practicals: Hands-On Training
(c) Self Work: Case Studies, Review of Literature, Home Assignment
presentation, discussion of various Case studies.
Assessment/ Examination Scheme:
Theory L/T (%)
Lab/Practical/Studio (%)
End Term Examination
60
40
100
Theory Assessment (L&T):
Components
(Drop down)
Weightage (%)
Class Test 1
5
Continuous Assessment/Internal Assessment (30)
Self-work
Home Assignment Seminar/ Presentation
10
5
5
End Term Examination
Attendance
5
70
Lab/ Practical/ Studio Assessment:
Components
(Drop down)
Weightage (%)
Continuous Assessment/Internal Assessment (40)
Performance Lab record
Viva
Attendance
20
10
5
5
End Term Examination (60)
Practical
Viva
40
20
Text & References:
• Salle AJ, Fundamental Principles of Bacteriology. (ISBN-10: 0070544808, ISBN-13: 9780070544802)
• Brook TD, Madigan M.T, Biology of Microorganisms. Prentice Hall Int. Inc. (ISBN-10: 0135208750, ISBN-13: 978-0135208755)
• Pelczar MJ, Chan ECS, Kreig NR Microbiology, Mc Graw Hill. (ISBN: 9780070492585)
• R. Ananthanarayanan and CK Jayaram Panicker (1997) Text of Microbiology, Orient Longman. (ISBN-13: 9788173716744, ISBN-10 : 8173716749)
Any other Study Material: Research Papers