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ProductInformation
N,N-Dimethylformamide
For molecular biology
CAT No
Grade
Application
Storage
C1396
BioUltra, ≥99.5%
Molecular biology
Room temperature
BioXtra, ≥99.0%
Molecular biology
Room temperature
Product Name:
N,N-Dimethylformamide
CAS Number:
68-12-2
Formula:
C3H7NO
Molecular weight:
73.10
Melting point:
- 61°C
Boiling point
153 °C
Density
0.9445 g/ml (25 °C)
Synonym:
DIMETHYLAMIDE; FORMDIMETHYLAMIDE; FORMYLDIMETHYLAMINE; DIMETHYLFORMAMIDE;
DMF; DMFA; N',N-DIMETHYLFORMAMIDE
Suitability
Dimethylformamide is an organic compound with the formula (CH3)2NC(O)H. Commonly abbreviated as DMF (although this
acronym is sometimes used for dimethylfuran), this colourless liquid is miscible with water and the majority of organic
liquids. DMF is a common solvent for chemical reactions. Dimethylformamide is odorless whereas technical grade or
degraded samples often have a fishy smell due to impurity of dimethylamine. As its name indicates, it is a derivative of
formamide, the amide of formic acid.
Dimethylformamide is a polar (hydrophilic) aprotic solvent with a high boiling point. It facilitates reactions that follow polar
mechanisms, such as SN2 reactions. Dimethylformamide can be synthesized from methyl formate anddimethylamine or by
reaction of dimethylamine with carbon monoxide. Dimethylformamide is reactive toward strong bases such as sodium
hydroxide or strong acids such as hydrochloric acid or sulfuric acid and is hydrolyzed back into formic acid and
dimethylamine, especially at elevated temperatures.
Applications
N, N-Dimethylformamide (DMF) is a solvent with a low evaporation rate, useful for preparing solutions with a variety of
hydrophobic organic compounds used in molecular biology applications. It was used to solubilize MTT crystals in cell
viability assays, and was also used in feruloyl esterase activity assay in molds exhibiting high activity of the enzyme.
This product has been tested to be suitable as a solvent for chromogenic substrates (X-Gal) in molecular biology applications
and as a solvent for MTT in molecular biology applications.
The primary use of dimethylformamide is as a solvent with low evaporation rate. DMF is used in the production of acrylic
fibers and plastics. It is also used as a solvent in peptide coupling for pharmaceuticals, in the development and production
of pesticides, and in the manufacture of adhesives, synthetic leathers, fibers, films, and surface coatings.
DMF is a polar organic solvent that is widely used in the synthesis of organic compounds. The high solven power and
stability of DMF at elevated temperatures contribute to its utility. DMF is a solvent for vinyl resins, acetylene, butadiene,
and polyacrylic fibers. It is also a catalyst in carboxylation reactions. DMF is also utilized in chromatographic applications
such as HPLC, capillary electrochromatography, and capillary electrophoresis. A capillary electrophoresis study has been
published on the mobility of large organic cations in such non-aqueous solvents as DMF. DMF has been used for the
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separation of aliphatic amines in microfabricated capillary electrophoresis devices. CNBr-activated diol-silica supports for
high performance affinity chromatography have been prepared with DMF. DMF has also been used for peptide and protein
crystallization.
Precautions and Disclaimer
For Laboratory Use Only. Not for drug, household or other uses.
Preparation Instructions
This product is freely miscible with water and with most common organic solvents, including alcohols, esters, ethers,
ketones, and chlorinated and aromatic hydrocarbons. The pH of a 0.5 M DMF aqueous solution is 6.7
Specification
Grade
BioUltra
Purity (GC)
Appearance (Form)
Appearance (Clarity)
Appearance (Color)
Infrared spectrum
UV Absorbance 400nm
UV Absorbance 340nm
UV Absorbance 310nm
Grade
 99.5 %
Liquid
Clear
Colorless
Conforms to Structure
≤ 0.01 _
≤ 0.01 _
≤ 0.05 _
Purity (GC)
Appearance (Form)
Infrared spectrum
Appearance (Color)
UV Absorbance 400nm
UV Absorbance 340nm
UV Absorbance 310nm
UV Absorbance 295nm
 99.0 %
Liquid
Conforms to Structure
Colorless
≤ 0.01
≤ 0.01
≤ 0.05
≤ 0.10
UV Absorbance 295nm
UV Absorbance 275nm
UV Absorbance 270nm
Color Test
Water (by Karl Fischer)
Residue on Evaporation
Titrable Acid (meq/g)
Titrable Base (meq/g)
≤ 0.10 _
≤ 0.30 _
≤ 1.0 _
≤ 15 APHA
≤ 0.15 %
≤ 0.005 %
≤ 0.0005
≤ 0.003
UV Absorbance 275nm
UV Absorbance 270nm
Water (by Karl Fischer)
Residue on Evaporation
Free Amines
Titrable Acid (meq/g)
Titrable Base (meq/g)
≤ 0.30
≤ 1.0
≤ 0.005 %
≤ 0.0005 %
≤ 8 ppm as Dimethylamine
≤ 0.0005
≤ 0.003
BioXltra
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