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Download Pegylated (12 kD) Interferon-α 2b (human)
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Pegylated (12 kD) Interferon-α 2b (human) PRODUCT INFORMATION Cat. No.: Z00395 Size: 20 ug or 1 mg or 100 ug Source: Escherichia coli. MW: 19,269 Dalton. Version: 09/27/2006 Description At least 23 different variants of Interferon-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end. Recombinant Human IFN-a 2b produced in E. coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19,269 Dalton. The Interferon-α 2b gene was obtained from human leukocytes. rHuPEG-IFN-α 2b is manufactured by attaching a 12,000 dalton methoxypolyethylene glycol-N-hydroxysuccinimid to IFN-a 2b having a total molecular mass of 31,269 dlaton. The rHuIFN-α 2b is purified by proprietary chromatographic techniques. Purity Greater than 98.0% as determined by: Analysis by SEC-HPLC using SEC column (Tosoh, TSK GEL Alpha series, G3000PWXL column). Flow rate: 1 ml/min. Solvent: Water. Elution time for conjugated PEG-IFN: ~5.5 min. Endotoxin Level Less than 0.1 ng/μg (IEU/μg) of IFN-α 2b. Quantitation Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IFN-α 2b as a Reference Standard. Specific Activity GenScript's pegylated IFN-α 2b is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 0.7X108 IU/mg. Storage Pegylated IFN α-2b although stable at 15 °C for 2 weeks, should be stored at 4 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Formulation Each ml contains 8 mg sodium chloride, 0.05 mg tween 80, 0.05 mg acetic acid and 2.62 mg sodium acetate pH6 and 10 mg benzyl alcohol. Sequence analysis The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln. N-terminal methionine has been completely removed enzymatically. ** For non-clinical research use only. ** Tel: 732-885-9188 Fax: 732-210-0262 E-Mail: [email protected] www.genscript.com
