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Pre/AP-GT Biology 2011
Biotechnology
Chapter 13 Genetic Engineering
Objective #6
Become familiar with modern genetic technology and its practical applications.
Explore the impact of research on society and the environment and compare genetic
variations in plants and animals
A. Intro to Genetic Engineering
1. What is selective breeding? Give an example of how humans have used selective breeding.
2. What is Luther Burbank’s contribution to science?
3. Burbank used hybridization to create variations of plants. What is hybridization?
4. What is inbreeding? What are the risks associated with inbreeding?
5. When a scientist wants to increase variation in a species, they will induce mutations. What does
“inducing mutations” mean and how does a scientist do this?
6. Give an example of a successful bacteria mutant and its impact on society.
7. Describe how polyploidy in plants occurs.
8. Compare a polyploidy plant’s characteristics to its diploid relative.
9. What is genetic engineering?
B.
1.
2.
3.
Recombinant DNA, etc
How is DNA extracted from a cell?
How is DNA “cut”? Define restriction enzyme.
After “cutting” the DNA the fragments are separated through the process of gel electrophoresis.
Describe this process.
4. What is recombinant DNA?
5. A DNA sequence from a human can be inserted into a bacterial plasmid. What is a plasmid?
6. Study figure 13-9. List the steps involved in transferring a human gene into a plasmid.
7. Once the new, recombinant plasmid containing human DNA is created, it is transferred into a
bacterial cell. What will that recombinant bacterial cell begin to produce?
8. How do you feel about the process of manipulating and recombining DNA from different organisms,
for example human/bacteria?
9. Read about GM foods p.330. Are stricter regulations needed?
10. What is biotechnology?
11. People with diabetes often require insulin injections. How is human insulin produced today using
biotechnology?
12. Study figure 13-12. Why is the plant glowing?
13. Biotechnology has improved the ability of plants to resist insects, allowing for fewer pesticides to
be used on crops. How?
14. What is a clone? What basic steps were followed to produce Dolly?
Activity # 1: PAPER PLASMID ACTIVITY – Making Recombinant DNA
Follow instructions and turn in completed activity to your teacher.
C. Go to http://learn.genetics.utah.edu/content/tech/genetherapy/ and briefly describe what you learn
after reading the information from each of the main links:
1a. WHAT IS GENE THERAPY ?
The what and why of gene therapy research.
1b. CHOOSING TARGETS FOR GENE THERAPY
See how researchers decide which disorders are appropriate for gene therapy.
1c. GENE DELIVERY: THE KEY TO GENE THERAPY
Putting therapeutic genes into cells is easier said than done. Find out why.
1d. CYSTIC FIBROSIS: CASE STUDY
An in depth look at the genetic disorder cystic fibrosis and the application of gene therapy as a
potential treatment
2. Complete the “space doctor” activity:
SPACE DOCTOR
You are the doctor! Design and test gene therapy treatments with ailing aliens
D. Go back to the main page http://learn.genetics.utah.edu/content/tech/genetherapy/ , scroll down and
click on:
WHAT ARE SOME ISSUES IN GENE THERAPY ?
Consider some important questions in the debate over gene therapy research.
Describe your response to one of the “questions to ponder”