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GENE CORRECTION’S INFLUENCE UPON THE KIDNEYS’ STRUCTURE IN
EXPERIMENTAL ATHEROSCLEROSIS
R.P.Piskun, O.A.Romashkina
Atherosclerosis is to be accompanied with the narrowing of vessels’ lumen followed by the
worsening of blood circulation and blood supply of various organs including kidneys. As a result
chronic kidney insufficiency appears due to kidney tissue sclerosis and nephron’s destruction. So
the vicious circle is formed because atherosclerosis stimulates hypertrophy development, and
hypertrophy promotes atherosclerosis. Gene therapy, an advanced biomedical technology, is
considered to be based on injection of gene construction to the cells for therapeutic purposes.
Actually, the injected gene is a new pharmaceutical drug which can be used for treatment of multi
factorial disease such as atherosclerosis. The achievements in molecular biology proved the
mutations of gene apolipoprotein E, the main protein of high density lipoproteins having
antiatherosclerotic properties, may cause lipoprotein metabolism disorders leading to the
development of atherosclerosis. The lack of integrated studying of kidneys structure in experimental
atherosclerosis and in condition of its gene correction determined the necessity of this research.
Materials and methods
The research was carried out in male rats of reproductive age. The model of atherosclerosis was
created with the help of cholesterin together with sunflower oil leading into rats’ stomach during 30
days in condition of thyroid gland function depression. The animals were distributed in 4 groups:
the first group involved the intact animals, the second one contained the rats with the experimental
modeling of atherosclerosis, the third one included the rats which obtained gene apolipoprotein E
for preventing purpose on the first day of experiment, the fourth group involved the animals which
obtained gene apolipoprotein E for therapeutic purpose on the fifteenth day of experiment.
Morphologic studying has been carried out on the histologic preparations painted by hematoxylin
and eosin, and according to Van Gieson’s method.
Results and discussion
Characteristic features of the first group of rats are the follows: renal corpuscles have the narrow
Bowman’s space, epithelium of the proximal convoluted tubules has the homogeneous cytoplasm,
renal corpuscles have a round or an oval shape. Characteristic features of the second group of rats
are the follows: contracted renal corpuscles have a wide Bowman’s space, kidney arteria’s wall is
thickened, there is the perivascular oedema, all these changes are local, and they are situated among
the normal structure areas. Characteristic features of the third group of rats are the follows: renal
corpuscles keep a round shape, Bowman’s space is narrow, little amount of renal corpuscles has the
enlarged Bowman’s space. Characteristic features of the fourth group of rats are the follows:
solitary renal corpuscles are reduced, and the rest of renal corpuscles have no structural changes,
kidney vessels are plethoric .
Conclusions
1. Pathologic changes in renal corpuscle structure accompanied by the injuring of knot of
capillaries and capsule’s extension as well as stroma’s oedema take place in the kidneys of rats with
the experimental modeling of atherosclerosis.
2. Gene apolipoprotein E correction promotes the positive effect in all components of nephron, so
this is evidence of regeneration processes in the target organ.
3. Data which have been obtained in case of the research prove the aspects for the further studying
of gene apolipoprotein E action in condition of the experimental modeling of atherosclerosis.