Derivatization - Sigma
... HPLC Derivatization Reagents for UV/VIS Detection .............................. 58 Reagents for Fluorescent Derivatives......................................................... 60 Reagents for Electrochemical Derivatives ............................................... 61 HPLC Derivatization Reagent ...
... HPLC Derivatization Reagents for UV/VIS Detection .............................. 58 Reagents for Fluorescent Derivatives......................................................... 60 Reagents for Electrochemical Derivatives ............................................... 61 HPLC Derivatization Reagent ...
Fulltext: english,
... Abstract. In this study, the electrochemical reduction and determination of oxcarbazepine were easily realized in various buffer solutions in the pH range of 4.50 to 11.15 in real samples using glassy carbon electrode (GCE) by cyclic voltammetric (CV) and differential pulse voltammetric (DPV) techni ...
... Abstract. In this study, the electrochemical reduction and determination of oxcarbazepine were easily realized in various buffer solutions in the pH range of 4.50 to 11.15 in real samples using glassy carbon electrode (GCE) by cyclic voltammetric (CV) and differential pulse voltammetric (DPV) techni ...
Supporting Information for Angew. Chem. Int. Ed. Z52444 © Wiley
... appropriate caution in a fume hood due to the flammable nature of mixtures of oxygen and organic solvents. Spectroscopic grade chloroform (Aldrich) or chloroform stabilized by amylenes was used without further purification. Chloroform stabilized by EtOH must be distilled prior to use. All other comm ...
... appropriate caution in a fume hood due to the flammable nature of mixtures of oxygen and organic solvents. Spectroscopic grade chloroform (Aldrich) or chloroform stabilized by amylenes was used without further purification. Chloroform stabilized by EtOH must be distilled prior to use. All other comm ...
Lab Manual Yr 1 organic
... The guidelines below are recommended for working safely in the laboratory. Know the location of all exits for the laboratory and the building. Know the location of the alarm and fire extinguishers and how to operate them. Know the location and use of safety showers, eye-washes and safety aid boxes. ...
... The guidelines below are recommended for working safely in the laboratory. Know the location of all exits for the laboratory and the building. Know the location of the alarm and fire extinguishers and how to operate them. Know the location and use of safety showers, eye-washes and safety aid boxes. ...
International Journal of
... atom. It is a clear to yellowish low melting solid, insoluble in water. Benzoxazole finds use in research as a starting material for the synthesis of larger, usually bioactive structures. It is found with in the chemical structures of pharmaceutical drugs such as flunoxa ...
... atom. It is a clear to yellowish low melting solid, insoluble in water. Benzoxazole finds use in research as a starting material for the synthesis of larger, usually bioactive structures. It is found with in the chemical structures of pharmaceutical drugs such as flunoxa ...
Liquid chromatography: a tool for the analysis of metal species
... separation column. The separation occurs according to their respective affinities towards the active sites of the separating resin. Ligands are also required to avoid precipitation when an acidic eluent is not suitable for the columns selected. A low-capacity silica-based cation-exchange column was ...
... separation column. The separation occurs according to their respective affinities towards the active sites of the separating resin. Ligands are also required to avoid precipitation when an acidic eluent is not suitable for the columns selected. A low-capacity silica-based cation-exchange column was ...
Gas Chromatography
... separation. However, helium has a larger range of flowrates that are comparable to hydrogen in efficiency, with the added advantage that helium is non-flammable, and works with a greater number of detectors. Therefore, helium is the most common carrier gas used. Detectoron are the flame ionization d ...
... separation. However, helium has a larger range of flowrates that are comparable to hydrogen in efficiency, with the added advantage that helium is non-flammable, and works with a greater number of detectors. Therefore, helium is the most common carrier gas used. Detectoron are the flame ionization d ...
ert207 analytical chemistry
... stationary phases, each of which can separate analytes according to different chemical properties. • Most stationary phases are based on silica that has been bonded to a specific functional group. ...
... stationary phases, each of which can separate analytes according to different chemical properties. • Most stationary phases are based on silica that has been bonded to a specific functional group. ...
Separation and Purification Methods
... high-performance liquid chromatography (HPLC), and thin-layer chromatography (TLC). Sample sizes for these procedures are usually quite small, from microgram to milligram quantities. In some cases, the chromatograph is coupled to another analytical instrument, such as a mass spectrometer or nuclear ...
... high-performance liquid chromatography (HPLC), and thin-layer chromatography (TLC). Sample sizes for these procedures are usually quite small, from microgram to milligram quantities. In some cases, the chromatograph is coupled to another analytical instrument, such as a mass spectrometer or nuclear ...
Paper chromatography
... polar SiOH groups at the surface of these adsorbents, and will tend to stick or adsorb onto the fine particles of the adsorbent while weakly polar molecules are held less tightly. • Weakly polar molecules generally tend to move through the adsorbent more rapidly than the polar species. Roughly, the ...
... polar SiOH groups at the surface of these adsorbents, and will tend to stick or adsorb onto the fine particles of the adsorbent while weakly polar molecules are held less tightly. • Weakly polar molecules generally tend to move through the adsorbent more rapidly than the polar species. Roughly, the ...
lab-6-chrmatography
... attracted differently to the porous stationary phase depending on its polarity and the polarity of the stationary phase chosen. Remember that “Like attracts Like”. If the stationary phase is polar then polar components will be attracted or stick more to it but non-polar components will move acro ...
... attracted differently to the porous stationary phase depending on its polarity and the polarity of the stationary phase chosen. Remember that “Like attracts Like”. If the stationary phase is polar then polar components will be attracted or stick more to it but non-polar components will move acro ...
Chromatography
... polar SiOH groups at the surface of these adsorbents, and will tend to stick or adsorb onto the fine particles of the adsorbent while weakly polar molecules are held less tightly. • Weakly polar molecules generally tend to move through the adsorbent more rapidly than the polar species. Roughly, the ...
... polar SiOH groups at the surface of these adsorbents, and will tend to stick or adsorb onto the fine particles of the adsorbent while weakly polar molecules are held less tightly. • Weakly polar molecules generally tend to move through the adsorbent more rapidly than the polar species. Roughly, the ...
High-performance liquid chromatography
High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out the column.HPLC has been used for medical (e.g. detecting vitamin D levels in blood serum), legal (e.g. detecting performance enhancement drugs in urine), research (e.g. separating the components of a complex biological sample, or of similar synthetic chemicals from each other), and manufacturing (e.g. during the production process of pharmaceutical and biological products) purposes.Chromatography can be described as a mass transfer process involving adsorption. HPLC relies on pumps to pass a pressurized liquid and a sample mixture through a column filled with a sorbent, leading to the separation of the sample components. The active component of the column, the sorbent, is typically a granular material made of solid particles (e.g. silica, polymers, etc.), 2–50 micrometers in size. The components of the sample mixture are separated from each other due to their different degrees of interaction with the sorbent particles. The pressurized liquid is typically a mixture of solvents (e.g. water, acetonitrile and/or methanol) and is referred to as a ""mobile phase"". Its composition and temperature play a major role in the separation process by influencing the interactions taking place between sample components and sorbent. These interactions are physical in nature, such as hydrophobic (dispersive), dipole–dipole and ionic, most often a combination.HPLC is distinguished from traditional (""low pressure"") liquid chromatography because operational pressures are significantly higher (50–350 bar), while ordinary liquid chromatography typically relies on the force of gravity to pass the mobile phase through the column. Due to the small sample amount separated in analytical HPLC, typical column dimensions are 2.1–4.6 mm diameter, and 30–250 mm length. Also HPLC columns are made with smaller sorbent particles (2–50 micrometer in average particle size). This gives HPLC superior resolving power (the ability to distinguish between compounds) when separating mixtures, which makes it a popular chromatographic technique.The schematic of an HPLC instrument typically includes a sampler, pumps, and a detector. The sampler brings the sample mixture into the mobile phase stream which carries it into the column. The pumps deliver the desired flow and composition of the mobile phase through the column. The detector generates a signal proportional to the amount of sample component emerging from the column, hence allowing for quantitative analysis of the sample components. A digital microprocessor and user software control the HPLC instrument and provide data analysis. Some models of mechanical pumps in a HPLC instrument can mix multiple solvents together in ratios changing in time, generating a composition gradient in the mobile phase. Various detectors are in common use, such as UV/Vis, photodiode array (PDA) or based on mass spectrometry. Most HPLC instruments also have a column oven that allows for adjusting the temperature the separation is performed at.