Project Title: Comparative genomics and transcriptomics to
... subsequent infection of patients has become a major concern in hospitals and health care facilities (8). A. baumannii strains have now been identified that are resistant to all known antimicrobials (26, 37). The one exacerbating factor in addition to the rapid spread of antimicrobial resistance is t ...
... subsequent infection of patients has become a major concern in hospitals and health care facilities (8). A. baumannii strains have now been identified that are resistant to all known antimicrobials (26, 37). The one exacerbating factor in addition to the rapid spread of antimicrobial resistance is t ...
The effect of higher protein human milk fortifier on growth in preterm
... was removed. Infants were weighed daily by care staff and weekly by trained research personnel. Length and head circumference – important measures of growth – were assessed weekly. Lean mass gain, which is better associated with adult metabolic health outcomes than adipose tissue gain in the in-hos ...
... was removed. Infants were weighed daily by care staff and weekly by trained research personnel. Length and head circumference – important measures of growth – were assessed weekly. Lean mass gain, which is better associated with adult metabolic health outcomes than adipose tissue gain in the in-hos ...
Classification of plant-pathogenic mycoplasma
... of recent papers on dot and Southern hybridization has contributed to our better understanding of the relatedness of the MLOs (Bertaccini et al., 1990; Bonnet et al., 1990; Lee & Davis, 1988; Lee et al., 1990; Kuske et al., 1991). Based on Southern hybridization with a DNA fragment of an MLO associa ...
... of recent papers on dot and Southern hybridization has contributed to our better understanding of the relatedness of the MLOs (Bertaccini et al., 1990; Bonnet et al., 1990; Lee & Davis, 1988; Lee et al., 1990; Kuske et al., 1991). Based on Southern hybridization with a DNA fragment of an MLO associa ...
The 100000 Genomes Project Protocol
... standards. In addition, Genomics England, and its partners will ensure that the tools provided within the secure infrastructure will both accelerate scientific progress and support the focused, interdisciplinary collaboration needed for clinical interpretation and patient benefit. To maximise the va ...
... standards. In addition, Genomics England, and its partners will ensure that the tools provided within the secure infrastructure will both accelerate scientific progress and support the focused, interdisciplinary collaboration needed for clinical interpretation and patient benefit. To maximise the va ...
Fragaria multicipita - DigitalCommons@University of Nebraska
... subgroups (putative taxa) that are classified within major 16S rRNA RFLP groups are distinguished on the basis of differences in one or few restriction sites in 16S rDNA (Lee et al. 1998) and ‘Candidatus Phytoplasma species’ have been designated on the basis of 16S rRNA gene sequences, it is importa ...
... subgroups (putative taxa) that are classified within major 16S rRNA RFLP groups are distinguished on the basis of differences in one or few restriction sites in 16S rDNA (Lee et al. 1998) and ‘Candidatus Phytoplasma species’ have been designated on the basis of 16S rRNA gene sequences, it is importa ...
Protein Purification by Inverse Transition Cycling
... ions and their concentrations). Examples of the effects of these two factors on the Tt for a given ELP construct are shown in Figures 2 and 3. Because the Tt decreases logarithmically with increasing concentration (Fig. 2), the effect of ELP concentration is of greatest practical importance at conce ...
... ions and their concentrations). Examples of the effects of these two factors on the Tt for a given ELP construct are shown in Figures 2 and 3. Because the Tt decreases logarithmically with increasing concentration (Fig. 2), the effect of ELP concentration is of greatest practical importance at conce ...
uk fetp core learning objectives
... field epidemiology work effectively, and will have attained the set of competencies that have agreed as core for field epidemiologists in the ...
... field epidemiology work effectively, and will have attained the set of competencies that have agreed as core for field epidemiologists in the ...
Preparation of PCR Products for DNA Sequencing
... into the Gel Nebulizer inside the Micropure separator (Figure 1). The device was capped and centrifuged for 10 min at 14 000× g. The filtrate from Micropure was sequenced without further purification or concentration. The agarose gels were prepared in different electrophoresis buffers to assess buff ...
... into the Gel Nebulizer inside the Micropure separator (Figure 1). The device was capped and centrifuged for 10 min at 14 000× g. The filtrate from Micropure was sequenced without further purification or concentration. The agarose gels were prepared in different electrophoresis buffers to assess buff ...
Looking for Darwin in Genomic Sequences—Validity and Success of
... In most cases, we lack prior knowledge of where in each gene positive selection may be acting. LRTs can still be validly used to identify genes showing previously unsuspected signals of selection—‘‘fishing expeditions’’ may actually find and catch fish. Given a significant test result, statistical m ...
... In most cases, we lack prior knowledge of where in each gene positive selection may be acting. LRTs can still be validly used to identify genes showing previously unsuspected signals of selection—‘‘fishing expeditions’’ may actually find and catch fish. Given a significant test result, statistical m ...
improving health through the use of genomic data
... ic data, and commercial activities will have a point of contact for building up innovation and business around genomic data. The systematic collection of genetic information has increased rapidly in several countries during recent years. In order for genetic information to be more widely used in hea ...
... ic data, and commercial activities will have a point of contact for building up innovation and business around genomic data. The systematic collection of genetic information has increased rapidly in several countries during recent years. In order for genetic information to be more widely used in hea ...
Domain-Based Benchmark Experiments
... A problem domain in the sense of this paper is collection of data sets. For a benchmark experiment the complete domain or a sample from the domain is available. Note that such a domain might even be indefinitely large, e.g., the domain of all fMRI images of human brains. Naturally, domain-based benc ...
... A problem domain in the sense of this paper is collection of data sets. For a benchmark experiment the complete domain or a sample from the domain is available. Note that such a domain might even be indefinitely large, e.g., the domain of all fMRI images of human brains. Naturally, domain-based benc ...
Lecture II: Genomic Methods
... Large SNP numbers requires automated procedure Recent algorithms – clustering/pooling strategies • Raw hybridization intensities normalized • Information combined across different samples at each SNP • Assign genotypes to entire clusters • For each sample, estimate probability of each of three genot ...
... Large SNP numbers requires automated procedure Recent algorithms – clustering/pooling strategies • Raw hybridization intensities normalized • Information combined across different samples at each SNP • Assign genotypes to entire clusters • For each sample, estimate probability of each of three genot ...
ACMG clinical laboratory standards for next
... Next-generation sequencing technologies have been and continue to be deployed in clinical laboratories, enabling rapid transformations in genomic medicine. These technologies have reduced the cost of large-scale sequencing by several orders of magnitude, and continuous advances are being made. It is ...
... Next-generation sequencing technologies have been and continue to be deployed in clinical laboratories, enabling rapid transformations in genomic medicine. These technologies have reduced the cost of large-scale sequencing by several orders of magnitude, and continuous advances are being made. It is ...
Co-evolving residues in membrane proteins
... and active sites (Gloor et al., 2005) allowing for the identification of functionally important regions in combination with entropic measures (Saraf et al., 2003). Lately, WW domain sequences that fold into stable structures and function like natural WW domains could be artificially engineered based ...
... and active sites (Gloor et al., 2005) allowing for the identification of functionally important regions in combination with entropic measures (Saraf et al., 2003). Lately, WW domain sequences that fold into stable structures and function like natural WW domains could be artificially engineered based ...
synthetic life - Biochemical Society
... Synthetic biology has a powerful vision for merging engineering design practice into the construction of biology systems and cells at the genetic level. It has led some commentators to predict a “Biotechnological Revolution” that will be transformative for the 21st century, but with such transformat ...
... Synthetic biology has a powerful vision for merging engineering design practice into the construction of biology systems and cells at the genetic level. It has led some commentators to predict a “Biotechnological Revolution” that will be transformative for the 21st century, but with such transformat ...
Whole Genome Sequencing versus Traditional Genotyping for
... lineage. The main performance outcome measure of whole genome sequencing (WGS) analyses was the degree of correlation of the WGS analyses with contact tracing data and the spatio-temporal distribution of the outbreak cases. WGS analyses of the 86 isolates revealed 85 single nucleotide polymorphisms ...
... lineage. The main performance outcome measure of whole genome sequencing (WGS) analyses was the degree of correlation of the WGS analyses with contact tracing data and the spatio-temporal distribution of the outbreak cases. WGS analyses of the 86 isolates revealed 85 single nucleotide polymorphisms ...
Laboratory Detection and Gene Cassette Stability of the
... The G170N mutation, ascribed to a CC to AA base pair substitution on positions 493-494 of the blaGES-2 coding region, distinguishes this ESBL from blaGES-1 and the blaIBC-type genes, making it an ideal target for developing a novel sequence-specific, peptide nucleic acid (PNA)-based, multiplex-PCR d ...
... The G170N mutation, ascribed to a CC to AA base pair substitution on positions 493-494 of the blaGES-2 coding region, distinguishes this ESBL from blaGES-1 and the blaIBC-type genes, making it an ideal target for developing a novel sequence-specific, peptide nucleic acid (PNA)-based, multiplex-PCR d ...
The Polymerase Chain Reaction (PCR) provides an extremely
... Sequencing methods - The process of determining the order of the nucleotide bases along a DNA strand is called DNA sequencing - In 1977 two separate methods for sequencing DNA were developed: the chain termination method or cycle sequencing (Sanger et al.) and the chemical degradation method or Maxa ...
... Sequencing methods - The process of determining the order of the nucleotide bases along a DNA strand is called DNA sequencing - In 1977 two separate methods for sequencing DNA were developed: the chain termination method or cycle sequencing (Sanger et al.) and the chemical degradation method or Maxa ...
For Print
... and Gil McVean. It has developed a unique analytical platform for genomic sequence data analysis and interpretation, and combines this with world-leading expertise in the analysis and data mining of large genomic databases. Genomics plc has already been awarded three grants from the U.K. Small Busin ...
... and Gil McVean. It has developed a unique analytical platform for genomic sequence data analysis and interpretation, and combines this with world-leading expertise in the analysis and data mining of large genomic databases. Genomics plc has already been awarded three grants from the U.K. Small Busin ...
S1 Text. Supplementary Methods
... We performed filtering of the assembly considered in analyses of allelic expression biases, to identify regions where we have high confidence in our SNP calls. To do so, we first identified genomic regions with evidence for large-scale copy-number variation, second, we identified repeats and selfish ...
... We performed filtering of the assembly considered in analyses of allelic expression biases, to identify regions where we have high confidence in our SNP calls. To do so, we first identified genomic regions with evidence for large-scale copy-number variation, second, we identified repeats and selfish ...
Archives of microbiology
... state, its optimal growth occurs at 10 % sucrose and pH 5.5 (Gillis et al. 1989; Stephan et al. 1991), and can fix nitrogen in the presence of 30 % sucrose at pH 3.0. However, its nitrogenase activity is partially inhibited by NH4+ at high sucrose concentrations, and it is not affected by the high c ...
... state, its optimal growth occurs at 10 % sucrose and pH 5.5 (Gillis et al. 1989; Stephan et al. 1991), and can fix nitrogen in the presence of 30 % sucrose at pH 3.0. However, its nitrogenase activity is partially inhibited by NH4+ at high sucrose concentrations, and it is not affected by the high c ...
Diapositiva 1
... Minisatellites are molecular marker loci consisting of tandem repeat units of a 10-50 base motif, flanked by conserved endonuclease restriction sites. They are detected by gel electrophoresis of restricted DNA and subsequent Southern blot hybridization to a radiolabeled DNA probe containing multiple ...
... Minisatellites are molecular marker loci consisting of tandem repeat units of a 10-50 base motif, flanked by conserved endonuclease restriction sites. They are detected by gel electrophoresis of restricted DNA and subsequent Southern blot hybridization to a radiolabeled DNA probe containing multiple ...
Saunders 1..13
... clinical symptoms might not be evident in newborns. Genetic screens performed on newborns are rapid, but are designed to unearth only a few genetic disorders, and serial gene sequencing is too slow to be clinically useful. Together, these complicating factors lead to the administration of treatments ...
... clinical symptoms might not be evident in newborns. Genetic screens performed on newborns are rapid, but are designed to unearth only a few genetic disorders, and serial gene sequencing is too slow to be clinically useful. Together, these complicating factors lead to the administration of treatments ...
Supplemental Methods Document Experimental design Soil s
... Amplicon sequences were analyzed using QIIME package [3]. Sequences were clustered into Operational Taxonomic Units (OTUs) using the pick_otus.py script and Uclust [4] method at 90% similarity. Some of the Anguil Bulk samples (9 samples, see Additional file 2) were excluded from the analysis as we ...
... Amplicon sequences were analyzed using QIIME package [3]. Sequences were clustered into Operational Taxonomic Units (OTUs) using the pick_otus.py script and Uclust [4] method at 90% similarity. Some of the Anguil Bulk samples (9 samples, see Additional file 2) were excluded from the analysis as we ...
Protein CVermont: Symptomatic Type I1 Protein C
... minutes of incubation in the dark, 50 p L of 4 mol/L H,SO, was added to each well to stop the color development. The plates were read at 490 nm. The dynamic range of the assay extended from 3% to 50% of the PIVKA protein C present in the warfarin plasma reference pool. Pooled plasma from patients on ...
... minutes of incubation in the dark, 50 p L of 4 mol/L H,SO, was added to each well to stop the color development. The plates were read at 490 nm. The dynamic range of the assay extended from 3% to 50% of the PIVKA protein C present in the warfarin plasma reference pool. Pooled plasma from patients on ...
Bioinformatics
Bioinformatics /ˌbaɪ.oʊˌɪnfərˈmætɪks/ is an interdisciplinary field that develops methods and software tools for understanding biological data. As an interdisciplinary field of science, bioinformatics combines computer science, statistics, mathematics, and engineering to analyze and interpret biological data.Bioinformatics is both an umbrella term for the body of biological studies that use computer programming as part of their methodology, as well as a reference to specific analysis ""pipelines"" that are repeatedly used, particularly in the fields of genetics and genomics. Common uses of bioinformatics include the identification of candidate genes and nucleotides (SNPs). Often, such identification is made with the aim of better understanding the genetic basis of disease, unique adaptations, desirable properties (esp. in agricultural species), or differences between populations. In a less formal way, bioinformatics also tries to understand the organisational principles within nucleic acid and protein sequences.