Fragaria multicipita - DigitalCommons@University of Nebraska
... primed by primer pair P1/P7 but from only rrnA in PCR primed by primer pair R16mF2/R16mR1. Preferential amplification of DNA from operon rrnA was explained by base mismatches between the R16mF2/R16mR1 primers and primer annealing sites in rrnB. The results revealed potential for classification of a ...
... primed by primer pair P1/P7 but from only rrnA in PCR primed by primer pair R16mF2/R16mR1. Preferential amplification of DNA from operon rrnA was explained by base mismatches between the R16mF2/R16mR1 primers and primer annealing sites in rrnB. The results revealed potential for classification of a ...
Biotechnology Timeline
... Japanese scientists create the first DNA molecule made almost entirely of artificial parts. This advances the field of gene therapy and brings scientists one step closer to creating an artificial organism. ...
... Japanese scientists create the first DNA molecule made almost entirely of artificial parts. This advances the field of gene therapy and brings scientists one step closer to creating an artificial organism. ...
Candidatus Liberibacter asiaticus - Southwest Florida Research and
... was transferred to an Eppendorf tube and incubated at 65°C for 30 min. Subsequently, one-third volume of 5 M potassium acetate was added, mixed thoroughly, and incubated on ice for 20 min. The mixture was centrifuged for 10 min at 13,000 rpm, and DNA was precipitated from 0.4 ml of supernatant by ad ...
... was transferred to an Eppendorf tube and incubated at 65°C for 30 min. Subsequently, one-third volume of 5 M potassium acetate was added, mixed thoroughly, and incubated on ice for 20 min. The mixture was centrifuged for 10 min at 13,000 rpm, and DNA was precipitated from 0.4 ml of supernatant by ad ...
This article was published in an Elsevier journal. The attached copy
... strain DY380 was obtained from Neal Copeland and Craig Stranthdee and used for mutagenesis. A Cre recombinase expression plasmid pGS403 was a gift from L. Enquist. 2.2. BAC methods 2.2.1. Generating ORF deletion mutants Primers were synthesized by Sigma-Genosys (Woodlands, TX) and stored in TE buffe ...
... strain DY380 was obtained from Neal Copeland and Craig Stranthdee and used for mutagenesis. A Cre recombinase expression plasmid pGS403 was a gift from L. Enquist. 2.2. BAC methods 2.2.1. Generating ORF deletion mutants Primers were synthesized by Sigma-Genosys (Woodlands, TX) and stored in TE buffe ...
Catabolic Plasmids - UQ eSpace
... system which detects the substrates of the degradative pathway. The regulatory genes, and sometimes the degradative genes, are expressed constitutively at a low level, but in the presence of substrate they activate the transcription of the degradative genes as well as enhancing their own expression. ...
... system which detects the substrates of the degradative pathway. The regulatory genes, and sometimes the degradative genes, are expressed constitutively at a low level, but in the presence of substrate they activate the transcription of the degradative genes as well as enhancing their own expression. ...
Preface 1 PDF
... development of powerful standard techniques, such as yeast two-hybrid, synthetic genetic array analysis, and tetrad analysis. Many proteins important in human biology were first discovered by studying their homologues in yeast, which include cell cycle proteins, signaling proteins and protein-process ...
... development of powerful standard techniques, such as yeast two-hybrid, synthetic genetic array analysis, and tetrad analysis. Many proteins important in human biology were first discovered by studying their homologues in yeast, which include cell cycle proteins, signaling proteins and protein-process ...
Ligation mediated PCR performed at low denaturation temperatures
... Melting temperatures of genomic DNA fragments obtained by digestion of restriction nuclease depends on their GC content and length. It is known that intervals of temperatures corresponding to the full transition from double- to singlestranded structure for a restriction DNA fragment may vary from ~0 ...
... Melting temperatures of genomic DNA fragments obtained by digestion of restriction nuclease depends on their GC content and length. It is known that intervals of temperatures corresponding to the full transition from double- to singlestranded structure for a restriction DNA fragment may vary from ~0 ...
Powerpoint summary
... They received the Nobel Prize in Medicine along with Wilkins and Franklin ...
... They received the Nobel Prize in Medicine along with Wilkins and Franklin ...
Table SI. Primers used for creation of the PHAC1co and
... UGTB1 -989Rev and PUGT1TRev_MluI. Additionally the 3’ end of the UGTB1coding sequence followed by the URA3 selectable marker was amplified from plasmid pGKO_ugtB1 (Saerens et al., 2011b) with primers URA3-677For_MluI and GTII +1296Rev. The purified PUGT1T fragment and selection marker were cut overn ...
... UGTB1 -989Rev and PUGT1TRev_MluI. Additionally the 3’ end of the UGTB1coding sequence followed by the URA3 selectable marker was amplified from plasmid pGKO_ugtB1 (Saerens et al., 2011b) with primers URA3-677For_MluI and GTII +1296Rev. The purified PUGT1T fragment and selection marker were cut overn ...
Additional file 5
... D) Hybridization signals associated with BpK96243 (left) and BtE264 (right) genomic DNA. See panel A) for details. E) Probe distributions of common and strain-specific probes. Red probes correspond to probes showing true signals only in the BpK96243 hybridization, purple probes to those common to bo ...
... D) Hybridization signals associated with BpK96243 (left) and BtE264 (right) genomic DNA. See panel A) for details. E) Probe distributions of common and strain-specific probes. Red probes correspond to probes showing true signals only in the BpK96243 hybridization, purple probes to those common to bo ...
Assay Standards Working Group Recommendations, November 2012
... In order to ensure that the data are reproducible, experiments should be performed with two or more biological replicates, unless there is a compelling reason indicating that this is impractical or wasteful (e.g. overlapping time points with high temporal resolution). A biological replicate is defin ...
... In order to ensure that the data are reproducible, experiments should be performed with two or more biological replicates, unless there is a compelling reason indicating that this is impractical or wasteful (e.g. overlapping time points with high temporal resolution). A biological replicate is defin ...
Agrobacterium
... plant, and tumors form. The ratio of auxin to cytokinin produced by the tumor genes determines the morphology of the tumor (root-like, disorganized or shoot-like). Agrobacterium in humans Although generally seen as an infection in plants, Agrobacterium can be responsible for opportunistic infections ...
... plant, and tumors form. The ratio of auxin to cytokinin produced by the tumor genes determines the morphology of the tumor (root-like, disorganized or shoot-like). Agrobacterium in humans Although generally seen as an infection in plants, Agrobacterium can be responsible for opportunistic infections ...
Assembly of microarrays for genome-wide measurement of
... amplification of mixtures of subclones from BACs, but found the ligation-mediated PCR procedure to be superior. Array printing. We used a custom built printer, employing a 4 x 4 array of quartz capillary tubes spaced on 3 mm centers to print ~70-100 μm diameter spots on 130 μm centers. We printed e ...
... amplification of mixtures of subclones from BACs, but found the ligation-mediated PCR procedure to be superior. Array printing. We used a custom built printer, employing a 4 x 4 array of quartz capillary tubes spaced on 3 mm centers to print ~70-100 μm diameter spots on 130 μm centers. We printed e ...
DNA Sequencing - Department of Computer Science
... A single “run” takes about 10 days to generate about 600 billion nucleotides of data Cost of the reagents is $5-10K per run; multiplexing (sequencing many samples per run) further reduces cost per genome ...
... A single “run” takes about 10 days to generate about 600 billion nucleotides of data Cost of the reagents is $5-10K per run; multiplexing (sequencing many samples per run) further reduces cost per genome ...
Lutz Heide, Pharmaceutical Institute, Tübingen University
... common type of phosphorylation in eukaryotes, on contrary, in bacteria phosphorylation occurs predominantly on histidine and aspartate (two-component system). Until the early 1990s it was largely considered that these two phosphorylation systems are mutually exclusive. Two-component systems: Crucial ...
... common type of phosphorylation in eukaryotes, on contrary, in bacteria phosphorylation occurs predominantly on histidine and aspartate (two-component system). Until the early 1990s it was largely considered that these two phosphorylation systems are mutually exclusive. Two-component systems: Crucial ...
Document
... Genomes can be modeled by permutations: each gene can be assigned a unique number and is exactly found once in the genome. ...
... Genomes can be modeled by permutations: each gene can be assigned a unique number and is exactly found once in the genome. ...
DNA Dots - miniPCR
... functions. This can take a lot of trial and error. But recently scientists have found a way to use the CRISPR/Cas9 system to solve this problem. The technique takes advantage of Cas9’s ability to work as DNA scissors that we can target to any gene as long as we can make RNA to guide it. Researchers ...
... functions. This can take a lot of trial and error. But recently scientists have found a way to use the CRISPR/Cas9 system to solve this problem. The technique takes advantage of Cas9’s ability to work as DNA scissors that we can target to any gene as long as we can make RNA to guide it. Researchers ...
Replication Deficient Viral Vectors - The Medical University of South
... Examples of High Risk Work with Viral Vectors Vector – Replication competent vector, capable of infecting humans Insert Gene – toxin or toxic at high levels, oncogene, immune modulation, increases viral tropism or pathogenicity Procedures – aerosol production (homogenization, vortexing in open tube ...
... Examples of High Risk Work with Viral Vectors Vector – Replication competent vector, capable of infecting humans Insert Gene – toxin or toxic at high levels, oncogene, immune modulation, increases viral tropism or pathogenicity Procedures – aerosol production (homogenization, vortexing in open tube ...
A Branch-and-Bound Method for the Multichromosomal
... of start and end points. Fig. 1 shows an example. We improve the basic branch-and-bound algorithm to enumerate each genome only once by using the canonical ordering. There are two states in the algorithm: (1) open chromosome and (2) build chromosome. The algorithm is in state “open chromosome” when ...
... of start and end points. Fig. 1 shows an example. We improve the basic branch-and-bound algorithm to enumerate each genome only once by using the canonical ordering. There are two states in the algorithm: (1) open chromosome and (2) build chromosome. The algorithm is in state “open chromosome” when ...
2008 BSHG newesletter 01
... If many different samples need to be analysed for the same region on a single run, then some means of identifying the sample derivation of each individual analysis needs to be employed. There are two main approaches available. Firstly, the sequencing array can be divided into sample specific regions ...
... If many different samples need to be analysed for the same region on a single run, then some means of identifying the sample derivation of each individual analysis needs to be employed. There are two main approaches available. Firstly, the sequencing array can be divided into sample specific regions ...
Recombinant Plasmids
... Recombinant Plasmids are extremely useful in society. It is because it is now possible to create unlimited amounts of whatever needed. These new cultured cells are used to manufacture ...
... Recombinant Plasmids are extremely useful in society. It is because it is now possible to create unlimited amounts of whatever needed. These new cultured cells are used to manufacture ...
Current Uses of Synthetic Biology for Chemicals
... requirement for hydrogen in the process. These simple processes enable the production of diesel from scalable renewable resources at a price competitive with petroleum (without subsidy). Synthetic biology has been essential in engineering the LS9 microbial catalysts. The biosynthetic pathways to pro ...
... requirement for hydrogen in the process. These simple processes enable the production of diesel from scalable renewable resources at a price competitive with petroleum (without subsidy). Synthetic biology has been essential in engineering the LS9 microbial catalysts. The biosynthetic pathways to pro ...
Using GenomiPhi DNA Amplification Kit for the Representative
... Distribution of High Consensus Sequence For Direct PCR from Soil Amplification Strategy ...
... Distribution of High Consensus Sequence For Direct PCR from Soil Amplification Strategy ...