Interactions of Annexins with the mu Subunits of the Clathrin
... To search for proteins that interact with the N-terminal domain of annexin A2, the N-terminal 34 residues were fused to the yeast GAL4 DNA binding domain and used as a bait in a yeast two-hybrid screen of a human fetal brain cDNA library. The bait construct was found not to cause autoactivation in c ...
... To search for proteins that interact with the N-terminal domain of annexin A2, the N-terminal 34 residues were fused to the yeast GAL4 DNA binding domain and used as a bait in a yeast two-hybrid screen of a human fetal brain cDNA library. The bait construct was found not to cause autoactivation in c ...
Cdc6 in S phase in human cells - Journal of Cell Science
... phase. A single round of DNA synthesis can be induced in nuclei from HeLa cells chemically synchronised in late G1 (Krude et al., 1997) or nuclei from mouse cells released from quiescence into G1 (Stoeber et al., 1998). In the latter case the proportion of nuclei which begin DNA synthesis is greatly ...
... phase. A single round of DNA synthesis can be induced in nuclei from HeLa cells chemically synchronised in late G1 (Krude et al., 1997) or nuclei from mouse cells released from quiescence into G1 (Stoeber et al., 1998). In the latter case the proportion of nuclei which begin DNA synthesis is greatly ...
Golgins and GTPases, giving identity and structure to the Golgi
... This doesn’t mean that they are unimportant. Rather, it probably indicates that the Golgi is structurally adapted in different species to meet the specific needs of different cell types. For example, the recent finding that GM130 is not required for mammalian cell viability at 34.5 8C, but is essent ...
... This doesn’t mean that they are unimportant. Rather, it probably indicates that the Golgi is structurally adapted in different species to meet the specific needs of different cell types. For example, the recent finding that GM130 is not required for mammalian cell viability at 34.5 8C, but is essent ...
Photoactivatable GFP tagging cassettes for protein
... Yeast cell biologists use a variety of fluorescent protein tags for determining protein localization and for measuring protein dynamics using fluorescence recovery after photobleaching (FRAP). Although many modern fluorescent proteins, such as those with photoactivatable and photoconvertible charact ...
... Yeast cell biologists use a variety of fluorescent protein tags for determining protein localization and for measuring protein dynamics using fluorescence recovery after photobleaching (FRAP). Although many modern fluorescent proteins, such as those with photoactivatable and photoconvertible charact ...
This article was published in an Elsevier journal. The attached copy
... from vegetative forms of Bacillus cereus. The purification procedure included ammonium sulphate fractionation, pH 4 treatment, ion exchange chromatography on DEAE-Sephacel, gel filtration on Sephacryl S-300 HR and affinity chromatography on N6-adenosyl agarose. The enzyme shows a good stability to b ...
... from vegetative forms of Bacillus cereus. The purification procedure included ammonium sulphate fractionation, pH 4 treatment, ion exchange chromatography on DEAE-Sephacel, gel filtration on Sephacryl S-300 HR and affinity chromatography on N6-adenosyl agarose. The enzyme shows a good stability to b ...
SERCA pump level is a critical determinant of Ca2+ homeostasis
... protein level was only modest above the endogenous expression. The basis for this observation is still not known. There might be competition between the exogenous and the endogenous protein and/or there are powerful post-transcriptional mechanisms working to maintain a physiological SERCA level. Sti ...
... protein level was only modest above the endogenous expression. The basis for this observation is still not known. There might be competition between the exogenous and the endogenous protein and/or there are powerful post-transcriptional mechanisms working to maintain a physiological SERCA level. Sti ...
Disruption of somitogenesis by a novel dominant allele of Lfng
... maintains the enzymatic activity of endogenous LFNG, but has a longer intracellular half-life than the wild-type LFNG protein (Shifley and Cole, 2008). We expressed RLFNG in vivo by replacing exon 1 of the endogenous Lfng locus with a new exon containing sequences encoding the N-terminus and type II ...
... maintains the enzymatic activity of endogenous LFNG, but has a longer intracellular half-life than the wild-type LFNG protein (Shifley and Cole, 2008). We expressed RLFNG in vivo by replacing exon 1 of the endogenous Lfng locus with a new exon containing sequences encoding the N-terminus and type II ...
Divergent Functional Properties of the Ribosome
... cross-linked to nascent polypeptide chains. Because Ssbs are members of a divergent subclass of Hsp70s found thus far only in fungi, we asked if the structural requirements for in vivo function were similar to those of “classic” Hsp70s. An intact peptide-binding domain is essential and an alteration ...
... cross-linked to nascent polypeptide chains. Because Ssbs are members of a divergent subclass of Hsp70s found thus far only in fungi, we asked if the structural requirements for in vivo function were similar to those of “classic” Hsp70s. An intact peptide-binding domain is essential and an alteration ...
The TRAPP Complex: Insights into its Architecture and
... and Bet3p shows that all the patches are remarkably conserved from yeast to humans (Figure 3). While the patch conservation supports the structural model, finer structure resolution and binding analysis of Ypt1p with relevant TRAPP I subunit mutations are required for nailing down the specific inter ...
... and Bet3p shows that all the patches are remarkably conserved from yeast to humans (Figure 3). While the patch conservation supports the structural model, finer structure resolution and binding analysis of Ypt1p with relevant TRAPP I subunit mutations are required for nailing down the specific inter ...
SURVEY AND SUMMARY H1 histones
... introns commonly observed in other protein-coding genes (85,86). Alternatively, somatic H1 genes contain a 30 stem-loop sequence allowing for rapid translation during DNA replication, while permitting tight regulation of gene expression after the conclusion of S phase (83,86,87). The expression patt ...
... introns commonly observed in other protein-coding genes (85,86). Alternatively, somatic H1 genes contain a 30 stem-loop sequence allowing for rapid translation during DNA replication, while permitting tight regulation of gene expression after the conclusion of S phase (83,86,87). The expression patt ...
The Platform Protein Is Essential for Type IV Pilus
... expression in Pseudomonas aeruginosa. By inactivating the retraction aspect of pilus function, genes essential for T4P assembly were discriminated. In contrast to previous studies in the T4P system of Neisseria spp., we found that components of the inner membrane subcomplex consisting of PilMNOP wer ...
... expression in Pseudomonas aeruginosa. By inactivating the retraction aspect of pilus function, genes essential for T4P assembly were discriminated. In contrast to previous studies in the T4P system of Neisseria spp., we found that components of the inner membrane subcomplex consisting of PilMNOP wer ...
Ribosomes slide on lysine-encoding homopolymeric A stretches
... eLife digest Genes provide the instructions to assemble proteins from smaller molecules called amino acids. When a gene is ‘switched on’, the DNA that makes up the gene is copied into messenger ribonucleic acid (or mRNA) molecules, composed of building blocks called nucleotides. There are four types ...
... eLife digest Genes provide the instructions to assemble proteins from smaller molecules called amino acids. When a gene is ‘switched on’, the DNA that makes up the gene is copied into messenger ribonucleic acid (or mRNA) molecules, composed of building blocks called nucleotides. There are four types ...
The TN5 Transposon - Biochemistry | UW-Madison
... Transposition is, in general, a quite rare, highly regulated process. Such tight regul~.tion mightenable the host cell to strike a balance betweeninsuring proliferation of the transposable element and insuring the cell’s owngenetic survival--the very process of transposition causes chromosome breaka ...
... Transposition is, in general, a quite rare, highly regulated process. Such tight regul~.tion mightenable the host cell to strike a balance betweeninsuring proliferation of the transposable element and insuring the cell’s owngenetic survival--the very process of transposition causes chromosome breaka ...
Increased and controlled expression of the Rickettsia
... an obligate exchange transport system that is specific for ATP and ADP, has been extremely difficult due to limited quantities of material available from these obligate intracytoplasmic bacteria and by the toxicity and poor expression in recombinant Escherichia coli expression systems. In this study ...
... an obligate exchange transport system that is specific for ATP and ADP, has been extremely difficult due to limited quantities of material available from these obligate intracytoplasmic bacteria and by the toxicity and poor expression in recombinant Escherichia coli expression systems. In this study ...
Reduced initiation frequency from oriC restores viability of a
... E. coli cells with reduced DnaA protein activity, due either to certain mutations in domain III or IV of the protein, or to the introduction of additional datA sites, initiate replication at an increased cell mass per origin. Initiations are often asynchronous, indicating that not all origins are in ...
... E. coli cells with reduced DnaA protein activity, due either to certain mutations in domain III or IV of the protein, or to the introduction of additional datA sites, initiate replication at an increased cell mass per origin. Initiations are often asynchronous, indicating that not all origins are in ...
Actin machinery: pushing the envelope Gary G Borisy* and Tatyana
... microbial rocketing have proved also to be involved in lamellipodial and filopodial protrusion. These sheet-like and spike-like processes, respectively, represent two different components of protrusive activity of crawling cells. Thus, the rocketing motion may be significant not only for understandi ...
... microbial rocketing have proved also to be involved in lamellipodial and filopodial protrusion. These sheet-like and spike-like processes, respectively, represent two different components of protrusive activity of crawling cells. Thus, the rocketing motion may be significant not only for understandi ...
Print - Circulation Research
... disease. Loss of phosphoinositide 3-kinase (PI3K)␥, the isoform linked to G protein– coupled receptor signaling, results in increased myocardial contractility, but the response to pressure overload is controversial. Objective: To characterize molecular and cellular responses of the PI3K␥ knockout (K ...
... disease. Loss of phosphoinositide 3-kinase (PI3K)␥, the isoform linked to G protein– coupled receptor signaling, results in increased myocardial contractility, but the response to pressure overload is controversial. Objective: To characterize molecular and cellular responses of the PI3K␥ knockout (K ...
ftsZ mutations affecting cell division frequency, placement and
... number of eukaryotic organelles, e.g. mitochondria and chloroplasts (reviewed by Vaughan et al., 2004). The crystal structure of FtsZ closely resembles that of eukaryotic a- and b-tubulin (Lowe & Amos, 1998; Nogales et al., 1998). FtsZ consists of two domains, of which the GTP-binding interactions l ...
... number of eukaryotic organelles, e.g. mitochondria and chloroplasts (reviewed by Vaughan et al., 2004). The crystal structure of FtsZ closely resembles that of eukaryotic a- and b-tubulin (Lowe & Amos, 1998; Nogales et al., 1998). FtsZ consists of two domains, of which the GTP-binding interactions l ...
Chloroplast envelope membranes: a dynamic interface between
... of lipids and terpenoid compounds serving numerous biochemical functions and the flexibility of their biosynthetic pathways allow plants to adapt to fluctuating environmental conditions (for instance phosphate deprivation). A large body of knowledge has been generated by proteomic studies targeted t ...
... of lipids and terpenoid compounds serving numerous biochemical functions and the flexibility of their biosynthetic pathways allow plants to adapt to fluctuating environmental conditions (for instance phosphate deprivation). A large body of knowledge has been generated by proteomic studies targeted t ...
BMP binding domains in the extracellular space
... homozygous for a truncated chordin allele lacking the three C-terminal CRs (CR2-4), but retaining CR1 and most of the inter-repeat region, were found to be viable hypomorphic mutants displaying only minor skeletal defects (D. B., J. Klingensmith, J. Rossant and E. M. D. R., unpublished results). Thi ...
... homozygous for a truncated chordin allele lacking the three C-terminal CRs (CR2-4), but retaining CR1 and most of the inter-repeat region, were found to be viable hypomorphic mutants displaying only minor skeletal defects (D. B., J. Klingensmith, J. Rossant and E. M. D. R., unpublished results). Thi ...
The Effect of Ultrasound on the Functional Properties of
... resulting from bubble size variation and subsequent collapse of bubbles. Commonly, strong microstreaming currents are associated with high-velocity gradients and shear stresses that can alter the characteristics of the liquid system [5]. The third aspect of the acoustic cavitation phenomenon is the ...
... resulting from bubble size variation and subsequent collapse of bubbles. Commonly, strong microstreaming currents are associated with high-velocity gradients and shear stresses that can alter the characteristics of the liquid system [5]. The third aspect of the acoustic cavitation phenomenon is the ...
Inherited defects in platelet signaling mechanisms
... responses to several agonists were impaired with the exception of thrombin [6]. The binding of TxA2 analogs to platelets was normal [6,7]. GTPase activity on activation with a TxA2 analog, but not thrombin, was diminished [7,8] suggesting a defect in TxA2 receptor-G-protein coupling. TxA2-induced ac ...
... responses to several agonists were impaired with the exception of thrombin [6]. The binding of TxA2 analogs to platelets was normal [6,7]. GTPase activity on activation with a TxA2 analog, but not thrombin, was diminished [7,8] suggesting a defect in TxA2 receptor-G-protein coupling. TxA2-induced ac ...
Natalia Gromak, Alexis Rideau,
... mutations at each of the three PTB consensus sites, while mutations of the DY pyrimidine tract involved complete deletion (DDY) or deletion of 12 nt including the two overlapping optimal PTB sites (DYDPC). As expected, compared with the wild-type construct all of the mutants had decreased amounts of ...
... mutations at each of the three PTB consensus sites, while mutations of the DY pyrimidine tract involved complete deletion (DDY) or deletion of 12 nt including the two overlapping optimal PTB sites (DYDPC). As expected, compared with the wild-type construct all of the mutants had decreased amounts of ...
The bicoid Protein Determines Position in the Drosophila Embryo in
... The analysis of maternal mutants also shows the autonomy of gradient interpretation. Medium and low protein levels are interpreted correctly regardless of whether high (exu) or very low (exu vasa) levels are present elsewhere in the embryo. This observed autonomy excludes the possibility that, for e ...
... The analysis of maternal mutants also shows the autonomy of gradient interpretation. Medium and low protein levels are interpreted correctly regardless of whether high (exu) or very low (exu vasa) levels are present elsewhere in the embryo. This observed autonomy excludes the possibility that, for e ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.