Enhancement of image quality and imaging depth with Airy light
... showing great promise in biomedical research. LSM enables rapid, high-contrast, optically sectioned visualization of large three-dimensional samples with minimal photo-damage and is therefore ideally suited for imaging studies in developmental biology [1] and neuroscience [2, 3]. Mammalian brains ar ...
... showing great promise in biomedical research. LSM enables rapid, high-contrast, optically sectioned visualization of large three-dimensional samples with minimal photo-damage and is therefore ideally suited for imaging studies in developmental biology [1] and neuroscience [2, 3]. Mammalian brains ar ...
Upholding the diffraction limit in the focusing of light and sound
... possible to resolve subwavelength features using near-field optical methods, in which case structures with subwavelength dimensions such as needles, tapered fibers or optical antennas are used to confine or scatter light [5,6]. An important development occurred in the year 2000, when Pendry [7] exte ...
... possible to resolve subwavelength features using near-field optical methods, in which case structures with subwavelength dimensions such as needles, tapered fibers or optical antennas are used to confine or scatter light [5,6]. An important development occurred in the year 2000, when Pendry [7] exte ...
Spectral Domain Optical Coherence Tomography
... Compared with traditional TDOCT, SDOCT technology yields unprecedented acquisition speeds and significantly higher axial resolutions using a potentially affordable SLD source. This system produced high quality images while operating at safe ocular exposure levels, even below that of the commercially ...
... Compared with traditional TDOCT, SDOCT technology yields unprecedented acquisition speeds and significantly higher axial resolutions using a potentially affordable SLD source. This system produced high quality images while operating at safe ocular exposure levels, even below that of the commercially ...
Spectroscopic study of chromium, iron, OH, fluid and mineral
... appear in the infrared and near-infrared and their energies are host-dependent. The NIR spectra of the two samples under study show (Fig. 3 and Fig. 5) overtone and combination stretching + bending modes of H2O around 7000 and 5200 cm−1, respectively [21 and 32]. The 5200 cm−1 overtone bands have lo ...
... appear in the infrared and near-infrared and their energies are host-dependent. The NIR spectra of the two samples under study show (Fig. 3 and Fig. 5) overtone and combination stretching + bending modes of H2O around 7000 and 5200 cm−1, respectively [21 and 32]. The 5200 cm−1 overtone bands have lo ...
polygonal mirror laser scanning heads: characteristic functions
... experimentally. Considerations on the design of PM scanning heads are extracted from the analysis. Key words: laser scanners, polygon mirrors, geometrical optics, multi-parametric analysis, swept sources, optical coherence tomography. ...
... experimentally. Considerations on the design of PM scanning heads are extracted from the analysis. Key words: laser scanners, polygon mirrors, geometrical optics, multi-parametric analysis, swept sources, optical coherence tomography. ...
PDF
... micron-thick sample by producing 10 frames in less than 5 ms. For repetitive processes, one can use only xy-scanning, refocusing it at different depth step-by step. The speed of imaging depends on many additional factors, such as needed contrast (integration time), size of the scanned area, camera a ...
... micron-thick sample by producing 10 frames in less than 5 ms. For repetitive processes, one can use only xy-scanning, refocusing it at different depth step-by step. The speed of imaging depends on many additional factors, such as needed contrast (integration time), size of the scanned area, camera a ...
Fourier Transforms and Images
... A function can be thought of as made up by adding sine waves. A well-known example is the Fourier series. To make a periodic function add up sine waves with wavelengths equal to the period divided by an integer. ...
... A function can be thought of as made up by adding sine waves. A well-known example is the Fourier series. To make a periodic function add up sine waves with wavelengths equal to the period divided by an integer. ...
Aalborg Universitet Plant Leaf Imaging using Time of Flight Camera
... soyabean leaves in the NIR region. This reflectance is low but in their findings on a frequency scale from 400 nm to 2500 nm, which also includes most of the visible spectrum, the only region having highest reflectance and lowest possible transmittance and absorption is the NIR region between 750 nm ...
... soyabean leaves in the NIR region. This reflectance is low but in their findings on a frequency scale from 400 nm to 2500 nm, which also includes most of the visible spectrum, the only region having highest reflectance and lowest possible transmittance and absorption is the NIR region between 750 nm ...
Introduction to Remote Sensing of Biomass
... place in what are known as spectral bands. A spectral band is defined as a discrete interval of the EM spectrum. For example the wavelength range of 0.4μm to 0.5μm (μm = micrometers or 10-6m) is one spectral band. Satellite sensors have been designed to measure responses within particular spectral b ...
... place in what are known as spectral bands. A spectral band is defined as a discrete interval of the EM spectrum. For example the wavelength range of 0.4μm to 0.5μm (μm = micrometers or 10-6m) is one spectral band. Satellite sensors have been designed to measure responses within particular spectral b ...
Shaping the focal intensity distribution using spatial coherence
... aperture is given by the Fourier transform of the correlation function. One such example is using an annular incoherent source to produce a J0-correlated field at the lens. The detailed three-dimensional structure of the light field in the focal region, however, requires a numerical solution of the ...
... aperture is given by the Fourier transform of the correlation function. One such example is using an annular incoherent source to produce a J0-correlated field at the lens. The detailed three-dimensional structure of the light field in the focal region, however, requires a numerical solution of the ...
Get PDF - OSA Publishing
... of stimulated emission depletion microscopy (STED) [1] and single molecule localization microscopy (SMLM) [2,3]. The winners, Stefan Hell, William Moerner, and Eric Betzig, were among the first to recognize that the classical resolution barrier in optical microscopy, formulated almost 150 years ago ...
... of stimulated emission depletion microscopy (STED) [1] and single molecule localization microscopy (SMLM) [2,3]. The winners, Stefan Hell, William Moerner, and Eric Betzig, were among the first to recognize that the classical resolution barrier in optical microscopy, formulated almost 150 years ago ...
(UAVs) as an Element of the Integrated Ocean Observing System
... only need high-resolution, near-realtime imagery that shows a clearly-defined, in space and time, plume of color in the ocean. This plume could be imaged using a panchromatic camera and enhanced through the use of filters or image processing techniques. It is this type of operational product that ca ...
... only need high-resolution, near-realtime imagery that shows a clearly-defined, in space and time, plume of color in the ocean. This plume could be imaged using a panchromatic camera and enhanced through the use of filters or image processing techniques. It is this type of operational product that ca ...
Microscopy Overview
... mirrors. For example, it may be a scanning imaging system. A confocal system is a combination of a conventional and a scanning system. Another important category of microscopes is that of probe microscopes, including near-field optical microscopes. Some probe microscopes, for example the atomic forc ...
... mirrors. For example, it may be a scanning imaging system. A confocal system is a combination of a conventional and a scanning system. Another important category of microscopes is that of probe microscopes, including near-field optical microscopes. Some probe microscopes, for example the atomic forc ...
Untitled
... Kenneth Barnard, US Air Force Research Laboratory, United States, Program Chair Pietro Ferraro, Istituto Nazionale di Ottica (CNR), Italy, Program Chair Kristina Irsch, Johns Hopkins University School of Medicine, United States, Program Chair Rajesh Menon, University of Utah, United States, Program ...
... Kenneth Barnard, US Air Force Research Laboratory, United States, Program Chair Pietro Ferraro, Istituto Nazionale di Ottica (CNR), Italy, Program Chair Kristina Irsch, Johns Hopkins University School of Medicine, United States, Program Chair Rajesh Menon, University of Utah, United States, Program ...
IASI level 0 and 1 processing algorithms description
... where N is the size of the FFT and is the off axis angle. This equation is valid directly for pixel of small size. For a larger pixel the angle must be averaged over the whole pixel. In order to be able to apply this spectral calibration, the value of the p angle must be known for each of the I ...
... where N is the size of the FFT and is the off axis angle. This equation is valid directly for pixel of small size. For a larger pixel the angle must be averaged over the whole pixel. In order to be able to apply this spectral calibration, the value of the p angle must be known for each of the I ...
PDF file. - UCL Medical Physics and Biomedical
... distribution of the absorption coefficient, and hence the local chromophore concentrations, from these images, it is necessary to use a mathematical model of light transport that is capable of calculating images of the absorbed optical energy density for arbitrary chromophore distributions. The mode ...
... distribution of the absorption coefficient, and hence the local chromophore concentrations, from these images, it is necessary to use a mathematical model of light transport that is capable of calculating images of the absorbed optical energy density for arbitrary chromophore distributions. The mode ...
Advanced Microscopy
... • smaple or the laser beam has to be moved (this makes the hole thing slow) moving the sample ...
... • smaple or the laser beam has to be moved (this makes the hole thing slow) moving the sample ...
Ultrahigh-resolution optical coherence tomography
... temporally low-coherence source is used to measure axial scattering from the sample. By scanning the beam across the tissue, a two-dimensional cross-sectional image of the internal tissue structure can be obtained. The coherence length of the light source gives the axial resolution, which is inverse ...
... temporally low-coherence source is used to measure axial scattering from the sample. By scanning the beam across the tissue, a two-dimensional cross-sectional image of the internal tissue structure can be obtained. The coherence length of the light source gives the axial resolution, which is inverse ...
MRI - Echo-planar Imaging - 81Bones.net
... Disadvantages of EPI • Highly susceptible to image artifacts – Nyquist ghosting: gradient-induced eddy currents cause timedependent frequency shifts, which in turn create phase differences from line to line in k-space because of the back and forth acquisition trajectories (this is exacerbated when ...
... Disadvantages of EPI • Highly susceptible to image artifacts – Nyquist ghosting: gradient-induced eddy currents cause timedependent frequency shifts, which in turn create phase differences from line to line in k-space because of the back and forth acquisition trajectories (this is exacerbated when ...
optical design of an echelle grating based atomic emission
... photo-multiplier detectors (PMT) is used for simultaneous multi-element analysis[1-2]. This type of instrument has several drawbacks such as the number of spectral lines recorded are limited due to physical limitations in mounting many exit slits. The instrument also lacks the flexibility of changin ...
... photo-multiplier detectors (PMT) is used for simultaneous multi-element analysis[1-2]. This type of instrument has several drawbacks such as the number of spectral lines recorded are limited due to physical limitations in mounting many exit slits. The instrument also lacks the flexibility of changin ...
Infrared IR Sensor Circuit Diagram and Working Principle
... This type of configuration is commonly referred to as non dispersive infrared (NDIR) technology. This type of analyzer is used for carbonated drinks. Non dispersive analyzer is used in most of the commercial IR instruments, for an automobile exhaust gas fuel leakages. ...
... This type of configuration is commonly referred to as non dispersive infrared (NDIR) technology. This type of analyzer is used for carbonated drinks. Non dispersive analyzer is used in most of the commercial IR instruments, for an automobile exhaust gas fuel leakages. ...
Clutter elimination for deep clinical optoacoustic imaging using
... journal homepage: www.elsevier.com/locate/pacs ...
... journal homepage: www.elsevier.com/locate/pacs ...
Medical applications of Terahertz Imaging: a Review of Current
... is absorption by water. The entire THz band is strongly absorbed by water (or any polar liquid) [9] and consequently does not penetrate moist tissue to any significant depth. It has been reported that a THz pulse can be detected through 1.5 mm of skin when the signal to noise ratio is 500:1 [10], th ...
... is absorption by water. The entire THz band is strongly absorbed by water (or any polar liquid) [9] and consequently does not penetrate moist tissue to any significant depth. It has been reported that a THz pulse can be detected through 1.5 mm of skin when the signal to noise ratio is 500:1 [10], th ...
Rejection of two-photon fluorescence background in
... According to Beer’s law, the proportion of ballistic light arriving at the focus decays roughly exponentially with focal depth in the media. Hence, the laser power must be increased exponentially to maintain a relatively constant TPEF signal level at increasing imaging depths, and the depth limitati ...
... According to Beer’s law, the proportion of ballistic light arriving at the focus decays roughly exponentially with focal depth in the media. Hence, the laser power must be increased exponentially to maintain a relatively constant TPEF signal level at increasing imaging depths, and the depth limitati ...
Hyperspectral imaging
Hyperspectral imaging, like other spectral imaging, collects and processes information from across the electromagnetic spectrum. The goal of hyperspectral imaging is to obtain the spectrum for each pixel in the image of a scene, with the purpose of finding objects, identifying materials, or detecting processes.Much as the human eye sees visible light in three bands (red, green, and blue), spectral imaging divides the spectrum into many more bands. This technique of dividing images into bands can be extended beyond the visible. In hyperspectral imaging, the recorded spectra have fine wavelength resolution and cover a wide range of wavelengths.Engineers build hyperspectral sensors and processing systems for applications in astronomy, agriculture, biomedical imaging, geosciences, physics, and surveillance. Hyperspectral sensors look at objects using a vast portion of the electromagnetic spectrum. Certain objects leave unique 'fingerprints' in the electromagnetic spectrum. Known as spectral signatures, these 'fingerprints' enable identification of the materials that make up a scanned object. For example, a spectral signature for oil helps geologists find new oil fields.