Synthesis and isolation of a single-strand
... a 1:1:1 ratio. This design imitates the proportion of the four nucleobases in a fully randomized library. Importantly, since the enzymes employed here may favor library members with little or no LNA content, our library design also ensures that all library members will have the same level of LNA mod ...
... a 1:1:1 ratio. This design imitates the proportion of the four nucleobases in a fully randomized library. Importantly, since the enzymes employed here may favor library members with little or no LNA content, our library design also ensures that all library members will have the same level of LNA mod ...
Evolutionary Origin and Adaptive Function of Meiosis
... under crowded conditions, such as in biofilms, continues to advance, many further examples of transformation are likely to be discovered. Transformation in bacteria can be viewed as a primitive sexual process, since it involves interaction of homologous DNA from two individ‐ uals to form recombinant ...
... under crowded conditions, such as in biofilms, continues to advance, many further examples of transformation are likely to be discovered. Transformation in bacteria can be viewed as a primitive sexual process, since it involves interaction of homologous DNA from two individ‐ uals to form recombinant ...
Control of Chromosome Pairing and Genome Evolution in Disomic
... probably indicate intergenomic chromosome associations and could have resulted in loss of RFLP fragments through subsequent segregation of recombined or broken chromosomes. A small frequency of these events could result in gain of novel fragments due to recombination events within the probed regions ...
... probably indicate intergenomic chromosome associations and could have resulted in loss of RFLP fragments through subsequent segregation of recombined or broken chromosomes. A small frequency of these events could result in gain of novel fragments due to recombination events within the probed regions ...
Crystal structure of the nucleosome core particle at 2.8 Å
... primary determinant of DNA accessibility. The physical properties of nucleosomes depend on solution conditions such as ionic strength, divalent-ion concentration, and on histone-modification state (4). For example, the variability of nucleosome spacing within in vitro-assembled arrays demonstrates t ...
... primary determinant of DNA accessibility. The physical properties of nucleosomes depend on solution conditions such as ionic strength, divalent-ion concentration, and on histone-modification state (4). For example, the variability of nucleosome spacing within in vitro-assembled arrays demonstrates t ...
Sheared DNA fragment sizing: comparison of techniques
... Fig. 4 Size Distribution of Sheared DNA Fragments. Panels a, b and c show the relative number of DNA molecules corresponding to given length increments (base pairs) as determined by Kleinschmidt EM, Adsorption EM and gel electrophoresis procedures, respectively. Panels d, e and f show the relative m ...
... Fig. 4 Size Distribution of Sheared DNA Fragments. Panels a, b and c show the relative number of DNA molecules corresponding to given length increments (base pairs) as determined by Kleinschmidt EM, Adsorption EM and gel electrophoresis procedures, respectively. Panels d, e and f show the relative m ...
LNA-PNA Comparison4
... DNA and RNA with high specificity and selectivity, leading to PNA–RNA and PNADNA hybrids more stable than the corresponding nucleic acid complexes. The thermal stability of PNA:DNA and PNA:RNA is higher compared to DNA:DNA and DNA:RNA duplexes. The stronger binding is attributed to the lack of charg ...
... DNA and RNA with high specificity and selectivity, leading to PNA–RNA and PNADNA hybrids more stable than the corresponding nucleic acid complexes. The thermal stability of PNA:DNA and PNA:RNA is higher compared to DNA:DNA and DNA:RNA duplexes. The stronger binding is attributed to the lack of charg ...
Multi-parent's niche: n-ary crossovers on NK-landscapes
... concluded that sexual recombination is useful for low and medium epistasis (not very rugged landscapes). We found that on these very problems a generalization of 1-point 2-parent crossover (that they use) to n-parent diagonal crossover increases GA performance. Also the results of Schaffer and Eshel ...
... concluded that sexual recombination is useful for low and medium epistasis (not very rugged landscapes). We found that on these very problems a generalization of 1-point 2-parent crossover (that they use) to n-parent diagonal crossover increases GA performance. Also the results of Schaffer and Eshel ...
The distribution of DNA translocation times in solid
... the nanopore to the positively biased trans chamber. The translocation of DNA molecules through a nanopore blocks the ionic current flow and produces transient ionic current blockages as shown in the pulses in figure 1(B). An integrated Axopatch 200B system (molecular devices) was used to perform th ...
... the nanopore to the positively biased trans chamber. The translocation of DNA molecules through a nanopore blocks the ionic current flow and produces transient ionic current blockages as shown in the pulses in figure 1(B). An integrated Axopatch 200B system (molecular devices) was used to perform th ...
Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides
... Complex between 1 and d(CCGGTACCGG) This self-complementary sequence crystallizes to give a symmetrical duplex with a stoichiometry of three cations of 1 per duplex, or 1.5 cations of 1 per decamer strand. All the nucleic acid strands are equivalent in the crystal lattice, with the packing shown in ...
... Complex between 1 and d(CCGGTACCGG) This self-complementary sequence crystallizes to give a symmetrical duplex with a stoichiometry of three cations of 1 per duplex, or 1.5 cations of 1 per decamer strand. All the nucleic acid strands are equivalent in the crystal lattice, with the packing shown in ...
An accessible database for mouse and human whole transcriptome
... is an important tool in quantitative studies of DNA and RNA molecules; especially in transcriptome studies, where different primer combinations allow identification of specific transcripts such as splice variants or precursor messenger RNA. Several softwares that implement various rules for optimal ...
... is an important tool in quantitative studies of DNA and RNA molecules; especially in transcriptome studies, where different primer combinations allow identification of specific transcripts such as splice variants or precursor messenger RNA. Several softwares that implement various rules for optimal ...
me-6 - Genetics
... the paired region. The Fixed Pairing Region model predicts that among prototrophic recombinants selected as random spores, the more frequent class having flanking markers of parental combination represents conversion of the mutant site further from an end of the pairing region; the hybrid-DNA model, ...
... the paired region. The Fixed Pairing Region model predicts that among prototrophic recombinants selected as random spores, the more frequent class having flanking markers of parental combination represents conversion of the mutant site further from an end of the pairing region; the hybrid-DNA model, ...
Genetic recombination in plants
... for genetic diversity through the generation of novel allelic arrays via crossing-over, a process which generates recombinant chromosomes. Recombination events can result in either reciprocal recombination or the related process of gene conversion. Reciprocal recombination arises via the exchange of ...
... for genetic diversity through the generation of novel allelic arrays via crossing-over, a process which generates recombinant chromosomes. Recombination events can result in either reciprocal recombination or the related process of gene conversion. Reciprocal recombination arises via the exchange of ...
D-Isonucleotide (isoNA) incorporation around cleavage site of
... complex.7 Then, the passenger strand is nicked between positions 9 and 10, counted from the 5′-terminal, by the Argonaute 2 (Ago2) protein in the same way as target mRNA is cleaved,8,9 followed by being degraded by C3PO to finally activate the RISC.10 Active RISC recognizes and cleaves complementary ...
... complex.7 Then, the passenger strand is nicked between positions 9 and 10, counted from the 5′-terminal, by the Argonaute 2 (Ago2) protein in the same way as target mRNA is cleaved,8,9 followed by being degraded by C3PO to finally activate the RISC.10 Active RISC recognizes and cleaves complementary ...
Southern molecular hybridization experiments with parallel
... may form perfect and stable parallel duplexes. Nonetheless, the results reported here suggests that parallel complementary oligonueleotides can be used successfully as probes in molecular hybridization experiments with cloned and genomic sequences, as well as for effective screening of genomic libra ...
... may form perfect and stable parallel duplexes. Nonetheless, the results reported here suggests that parallel complementary oligonueleotides can be used successfully as probes in molecular hybridization experiments with cloned and genomic sequences, as well as for effective screening of genomic libra ...
as a PDF - CiteSeerX
... homologous recombination and is also likely to affect recombination between diverged DNAs. For example, altered semiconservative replication in yeast DNA polymerase mutants can increase homologous recombination severalfold in a variety of systems, although the reason remains unclear (3, 28). Long in ...
... homologous recombination and is also likely to affect recombination between diverged DNAs. For example, altered semiconservative replication in yeast DNA polymerase mutants can increase homologous recombination severalfold in a variety of systems, although the reason remains unclear (3, 28). Long in ...
DNA Self-assembly Model for Matrix Addition Problem
... column of an L-configuration. The adder adds one bit of the column number to the row number, per row. The i th bit has to be added at the ith position, and the system uses the yellow diagonal line to compute that position. Each tile has two input sides (south and east) and two output sides (north an ...
... column of an L-configuration. The adder adds one bit of the column number to the row number, per row. The i th bit has to be added at the ith position, and the system uses the yellow diagonal line to compute that position. Each tile has two input sides (south and east) and two output sides (north an ...
Site-Directed Mutagenesis Using Oligonucleotide
... 1.2 Use of λ Red recombination for manipulation of bacterial genomes The basic strategy of the λ Red system is the replacement of a chromosomal sequence with a (e.g., PCR-amplified) selectable antibiotic resistance gene flanked by homology extensions of distinct lengths. For genetic engineering in t ...
... 1.2 Use of λ Red recombination for manipulation of bacterial genomes The basic strategy of the λ Red system is the replacement of a chromosomal sequence with a (e.g., PCR-amplified) selectable antibiotic resistance gene flanked by homology extensions of distinct lengths. For genetic engineering in t ...
The Large Loop Repair and Mismatch Repair Pathways
... Plasmids containing his4 alleles with varying length DNA sequence insertions were used to replace the wild-type HIS4 chromosomal sequence in AS13. Each plasmid was constructed by annealing two complementary oligonucleotides and inserting the oligos into the Sal I site in HIS4 on pDN9 (Nag et al. 198 ...
... Plasmids containing his4 alleles with varying length DNA sequence insertions were used to replace the wild-type HIS4 chromosomal sequence in AS13. Each plasmid was constructed by annealing two complementary oligonucleotides and inserting the oligos into the Sal I site in HIS4 on pDN9 (Nag et al. 198 ...
Tertiary base pair interactions in slipped loop-DNA
... in length, which cannot fold as a standard hairpin but which may favor the SLS formation. AT base pairs were identified, consistent only with the formation of an additional, tertiary miniduplex in the SLS. An all-atom stereochemically sound model has been developed for the SLS with the use of confor ...
... in length, which cannot fold as a standard hairpin but which may favor the SLS formation. AT base pairs were identified, consistent only with the formation of an additional, tertiary miniduplex in the SLS. An all-atom stereochemically sound model has been developed for the SLS with the use of confor ...
Automated Constraint-Based Nucleotide Sequence Selection for
... To simplify the design of our computational systems, we have developed a tool for constraint-based selection of nucleotide sequences. This tool incorporates domain knowledge that has proven to be important in our experimental process. However, we have also formulated a framework for systematically s ...
... To simplify the design of our computational systems, we have developed a tool for constraint-based selection of nucleotide sequences. This tool incorporates domain knowledge that has proven to be important in our experimental process. However, we have also formulated a framework for systematically s ...
PcrA Helicase Tightly Couples ATP Hydrolysis to Unwinding Double
... (Figure 3a). Anisotropy is dependent on the rotational correlation time of the fluorophore, which is often dependent on the molecular mass of its complex, thus increasing as RepD binds to the DNA. A titration of RepD into a solution of Junction 1 (DNA is defined in Figure 2) is shown in Figure 3a. T ...
... (Figure 3a). Anisotropy is dependent on the rotational correlation time of the fluorophore, which is often dependent on the molecular mass of its complex, thus increasing as RepD binds to the DNA. A titration of RepD into a solution of Junction 1 (DNA is defined in Figure 2) is shown in Figure 3a. T ...
Gel Electrophoresis
... • Lithium borate (LB) is relatively new but it has low conductivity, so much higher voltage could be used, which means a shorter analysis time for routine electrophoresis. ...
... • Lithium borate (LB) is relatively new but it has low conductivity, so much higher voltage could be used, which means a shorter analysis time for routine electrophoresis. ...
MORE ABOUT SOLUTIONS - Bio-Link
... In many ways, DNA is structurally and functionally simpler than protein ...
... In many ways, DNA is structurally and functionally simpler than protein ...
DNA Mutation and Repair
... • Double-strand breaks are repaired via two mechanisms • Synthesis dependent strand annealing ...
... • Double-strand breaks are repaired via two mechanisms • Synthesis dependent strand annealing ...
Full-Text PDF
... to permit virus replication in a controlled or limited manner in vivo (for a review see [1]). While many early studies based on these techniques were successful, the scope of determining viral gene function was limited by two factors. Firstly, genes could only be manipulated using homologous recombi ...
... to permit virus replication in a controlled or limited manner in vivo (for a review see [1]). While many early studies based on these techniques were successful, the scope of determining viral gene function was limited by two factors. Firstly, genes could only be manipulated using homologous recombi ...
Holliday junction
A Holliday junction is a branched nucleic acid structure that contains four double-stranded arms joined together. These arms may adopt one of several conformations depending on buffer salt concentrations and the sequence of nucleobases closest to the junction. The structure is named after the molecular biologist Robin Holliday, who proposed its existence in 1964.In biology, Holliday junctions are a key intermediate in many types of genetic recombination, as well as in double-strand break repair. These junctions usually have a symmetrical sequence and are thus mobile, meaning that the four individual arms may slide though the junction in a specific pattern that largely preserves base pairing. Additionally, four-arm junctions similar to Holliday junctions appear in some functional RNA molecules.Immobile Holliday junctions, with asymmetrical sequences that lock the strands in a specific position, were artificially created by scientists to study their structure as a model for natural Holliday junctions. These junctions also later found use as basic structural building blocks in DNA nanotechnology, where multiple Holliday junctions can be combined into specific designed geometries that provide molecules with a high degree of structural rigidity.