Par-1
... machinery Many components of RNAi machinery have been identified through genetic screening for RNAi defective mutants and through biochemical studies using cell extracts (e.g. Drosophila embryo extract). ...
... machinery Many components of RNAi machinery have been identified through genetic screening for RNAi defective mutants and through biochemical studies using cell extracts (e.g. Drosophila embryo extract). ...
p68/DDX5 DEAD-box RNA helicase gene encodes a novel miRNA
... that while p68 RNA helicase activity appears to be important for some functions (e.g., RNA processing), it does not appear to be required for its role as a transcriptional coactivator. The p68 gene contains a large intron (intron 11, 1.2 kb in the human gene), which has been conserved through evolut ...
... that while p68 RNA helicase activity appears to be important for some functions (e.g., RNA processing), it does not appear to be required for its role as a transcriptional coactivator. The p68 gene contains a large intron (intron 11, 1.2 kb in the human gene), which has been conserved through evolut ...
Alternatively Spliced Genes
... 3 orientation of U4 and U4atac is from right to left, and that of U1, U11, U2, U12, and U5snRNAs is from left to right. The RNA helices are indicated by Roman numerals, and nucleotide positions by Arabic numerals. The shaded nucleotides are binding sites for Sm proteins, and regions marked with bla ...
... 3 orientation of U4 and U4atac is from right to left, and that of U1, U11, U2, U12, and U5snRNAs is from left to right. The RNA helices are indicated by Roman numerals, and nucleotide positions by Arabic numerals. The shaded nucleotides are binding sites for Sm proteins, and regions marked with bla ...
Structural Analyses of Zinc Finger Domains for Specific Interactions
... interactions with basic residues are an interesting primary structure and a main reason that GAGA binds specifically to GAGA-rich sequences with one zinc finger domain. The basic residues, including Lys13, Arg14, and Lys23, generate contacts with the minor groove. There is an extended α-helix (Arg27 ...
... interactions with basic residues are an interesting primary structure and a main reason that GAGA binds specifically to GAGA-rich sequences with one zinc finger domain. The basic residues, including Lys13, Arg14, and Lys23, generate contacts with the minor groove. There is an extended α-helix (Arg27 ...
Computational Definition of
... We have used a computational approach to identify ESEs and ESSs associated with constitutively spliced exons. We focused on constitutive splicing as opposed to alternative splicing so as to tackle the more fundamental problem represented by the former and to avoid what are likely more complex mechan ...
... We have used a computational approach to identify ESEs and ESSs associated with constitutively spliced exons. We focused on constitutive splicing as opposed to alternative splicing so as to tackle the more fundamental problem represented by the former and to avoid what are likely more complex mechan ...
Syntabulin, a motor protein linker, controls dorsal
... disorganized vegetal microtubule array formation, and subsequently displays ventralization (Mei et al., 2009). Furthermore, a ubiquitin ligase, tripartite motif-containing 36 (trim36), is localized to the vegetal cortex of Xenopus oocytes, and when depleted causes defective vegetal microtubule array ...
... disorganized vegetal microtubule array formation, and subsequently displays ventralization (Mei et al., 2009). Furthermore, a ubiquitin ligase, tripartite motif-containing 36 (trim36), is localized to the vegetal cortex of Xenopus oocytes, and when depleted causes defective vegetal microtubule array ...
Translational control of regA, a key gene controlling
... copies of the 12 nucleotide BamHI linker sequence CGCGGATCCGCG and inserted it ~50 bp upstream of the E3 landing site (Fig. 2) according to Hallmann and Wodniok (Hallmann and Wodniok, 2006). To facilitate cloning, an additional SalI site (GTCGAC) was introduced into the central loop of the hairpin ( ...
... copies of the 12 nucleotide BamHI linker sequence CGCGGATCCGCG and inserted it ~50 bp upstream of the E3 landing site (Fig. 2) according to Hallmann and Wodniok (Hallmann and Wodniok, 2006). To facilitate cloning, an additional SalI site (GTCGAC) was introduced into the central loop of the hairpin ( ...
informe tecnológico de patentes
... disorder caused by the expression of mutant huntingtin protein (Htt). Suppression of Htt expression, using RNA interference, might be an effective therapy. However, if reduction of wild-type protein is not well tolerated in the brain, it may be necessary to suppress just the product of the mutant al ...
... disorder caused by the expression of mutant huntingtin protein (Htt). Suppression of Htt expression, using RNA interference, might be an effective therapy. However, if reduction of wild-type protein is not well tolerated in the brain, it may be necessary to suppress just the product of the mutant al ...
Personal details Current position Name: Lilah Glazer, PhD E-mail:
... documented worldwide and are a cause for concern for both human health and wildlife conservation. Over the past several years there has been increased recognition that the earlylife environment can strongly influence the trajectory of developmental pathways, and that perturbations at critical stages ...
... documented worldwide and are a cause for concern for both human health and wildlife conservation. Over the past several years there has been increased recognition that the earlylife environment can strongly influence the trajectory of developmental pathways, and that perturbations at critical stages ...
Penagarikano O, Mulle JG, and Warren ST: The Pathophysiology of Fragile X Syndrome; Annual Rev. Genomics Hum. Genet., 8:109-29 (2007).
... The location of the principal domains of the normally encoded protein is also indicated. ...
... The location of the principal domains of the normally encoded protein is also indicated. ...
[PDF]
... premutation-size alleles in their sperm (see below) (26). In the full mutation, the CGG repeat is massively expanded and hypermethylated, which results in the repression of the FMR1 gene and leads to the loss of FMRP. However, mosaicism of both CGG repeat length and methylation status exists in frag ...
... premutation-size alleles in their sperm (see below) (26). In the full mutation, the CGG repeat is massively expanded and hypermethylated, which results in the repression of the FMR1 gene and leads to the loss of FMRP. However, mosaicism of both CGG repeat length and methylation status exists in frag ...
Abundant RNA editing sites of chloroplast protein
... and it mostly converts C to U, although hornwort and fern have abundant U to C editing. Moreover, the editing events have also been detected in tRNAs, introns and the untranslated regions [19, 20]. RNA editing is essential for the normal development of plant and is involved in a wide variety of biol ...
... and it mostly converts C to U, although hornwort and fern have abundant U to C editing. Moreover, the editing events have also been detected in tRNAs, introns and the untranslated regions [19, 20]. RNA editing is essential for the normal development of plant and is involved in a wide variety of biol ...
LS1a Fall 2014 Lab 6: Ribosomal Protein Translation (PyMOL lab #3)
... of a sequence of ribonucleotides in a strand of messenger RNA (“mRNA”) into the correct sequence of amino acids of a protein. The ribosome reads the mRNA transcript in the 5’ 3’ direction, and synthesizes an amino acid polymer (a “polypeptide”) in the amino-terminal (“Nterminal”) to carboxy-termin ...
... of a sequence of ribonucleotides in a strand of messenger RNA (“mRNA”) into the correct sequence of amino acids of a protein. The ribosome reads the mRNA transcript in the 5’ 3’ direction, and synthesizes an amino acid polymer (a “polypeptide”) in the amino-terminal (“Nterminal”) to carboxy-termin ...
Multistep process of FUS aggregation in the cell cytoplasm involves
... in detergent-insoluble fraction (Fig. 3D) and formed structures resembling aggregates in cells expressing FUS R522G (Fig. 3B). In contrast, FUS-Npl3 chimeric protein was found only in soluble fraction (Fig. 3D) and its distribution was exclusively diffuse regardless of expression levels (Fig. 3B and ...
... in detergent-insoluble fraction (Fig. 3D) and formed structures resembling aggregates in cells expressing FUS R522G (Fig. 3B). In contrast, FUS-Npl3 chimeric protein was found only in soluble fraction (Fig. 3D) and its distribution was exclusively diffuse regardless of expression levels (Fig. 3B and ...
Thesis-1965R-K29r
... composition of the nucleolus is chiefly RNA and proteins. It may also contain certain enzymes and I ipids. The synthesis of RNA in the nucleolus is generally accepted. The types are not agreed on. The transfer of RNA to the cytoplasm seems probable but the method is not apparent. Protein synthesis w ...
... composition of the nucleolus is chiefly RNA and proteins. It may also contain certain enzymes and I ipids. The synthesis of RNA in the nucleolus is generally accepted. The types are not agreed on. The transfer of RNA to the cytoplasm seems probable but the method is not apparent. Protein synthesis w ...
Supplemental figure legends13092010HM
... (Topf et al, 2008). The fitting procedure for the apo exosome improved the crosscorrelation coefficient between the cryo-EM map and the pseudo-atomic model from 0.54 for a rigid body fit of the crystal structures to 0.62 for the final model after flexible fitting. Fitting of the RNA-bound exosome wa ...
... (Topf et al, 2008). The fitting procedure for the apo exosome improved the crosscorrelation coefficient between the cryo-EM map and the pseudo-atomic model from 0.54 for a rigid body fit of the crystal structures to 0.62 for the final model after flexible fitting. Fitting of the RNA-bound exosome wa ...
Gene Section FA1 (Fanconi anaemia 1) Atlas of Genetics and Cytogenetics
... Verlander PC, Auerbach AD. Sequence variation in the Fanconi anemia gene FAA. Proc Natl Acad Sci USA ...
... Verlander PC, Auerbach AD. Sequence variation in the Fanconi anemia gene FAA. Proc Natl Acad Sci USA ...
An Introduction to RNA Interference (RNAi)
... endogenously encoded primary miRNA transcripts in the cell nucleus that are processed into precursor microRNA and then exported from the nucleus into the cytoplasm. Here, the Dicer enzyme complex further processes them for RISC loading. Unlike siRNAs, mature miRNAs typically have imperfect sequence ...
... endogenously encoded primary miRNA transcripts in the cell nucleus that are processed into precursor microRNA and then exported from the nucleus into the cytoplasm. Here, the Dicer enzyme complex further processes them for RISC loading. Unlike siRNAs, mature miRNAs typically have imperfect sequence ...
Virp1 Is a Host Protein with a Major Role in Potato - IMBB
... circular positive-strand RNA into multimeric, linear negative strands. The latter then serve as the replication intermediates for enhanced production of multimeric, linear positive strands that are finally processed into unit-length, mature circular RNAs (1). While the negative strands are anchored ...
... circular positive-strand RNA into multimeric, linear negative strands. The latter then serve as the replication intermediates for enhanced production of multimeric, linear positive strands that are finally processed into unit-length, mature circular RNAs (1). While the negative strands are anchored ...
Nonsensemediated decay of glutathione peroxidase 1 mRNA in the
... ef®ciency with which the selenocysteine (Sec) UGA codon is recognized as nonsense either by lowering the intracellular selenium concentration, which reduces the level of charged selenocysteyl-tRNA (Hat®eld et al., 1991), or by changing the Sec codon to nonsense (UAA) increases the ef®ciency of NMD; ...
... ef®ciency with which the selenocysteine (Sec) UGA codon is recognized as nonsense either by lowering the intracellular selenium concentration, which reduces the level of charged selenocysteyl-tRNA (Hat®eld et al., 1991), or by changing the Sec codon to nonsense (UAA) increases the ef®ciency of NMD; ...
Increased transcription rates correlate with increased reversion rates
... transcription in derepressed genes were correlated with increased reversion rates. Rates of leuB and argH mRNA synthesis were determined using half-lives and concentrations, during exponential growth and at several time points during 30 min of amino acid starvation. Changes in mRNA concentration wer ...
... transcription in derepressed genes were correlated with increased reversion rates. Rates of leuB and argH mRNA synthesis were determined using half-lives and concentrations, during exponential growth and at several time points during 30 min of amino acid starvation. Changes in mRNA concentration wer ...
Solid Tumour Section Head and Neck: Retinoma Atlas of Genetics and Cytogenetics
... is more common in children with retinoma than in adults. In a child, the physician may opt to treat retinoma as retinoblastoma if there is even a minor suspicion of malignant progression. ...
... is more common in children with retinoma than in adults. In a child, the physician may opt to treat retinoma as retinoblastoma if there is even a minor suspicion of malignant progression. ...
CHAPTER 16
... Chapter 19 describes a blotting method known as Northern blotting, which can be used to detect RNA that is transcribed from a particular gene or a particular operon. In this method, a specific RNA is detected by using a short segment of cloned DNA as a probe. The DNA probe, which is radioactive, is ...
... Chapter 19 describes a blotting method known as Northern blotting, which can be used to detect RNA that is transcribed from a particular gene or a particular operon. In this method, a specific RNA is detected by using a short segment of cloned DNA as a probe. The DNA probe, which is radioactive, is ...
Article On the Complexity of Chloroplast RNA
... translation rely on a large cohort of nucleus-encoded proteins that act specifically on a single target transcript or a small set of targets. For example in the chloroplast of Chlamydomonas, trans-splicing of the two split introns of psaA requires at least 14 nucleus-encoded proteins. To investigate ...
... translation rely on a large cohort of nucleus-encoded proteins that act specifically on a single target transcript or a small set of targets. For example in the chloroplast of Chlamydomonas, trans-splicing of the two split introns of psaA requires at least 14 nucleus-encoded proteins. To investigate ...
Bicoid associates with the 5 -cap-bound complex of caudal mRNA
... Translational control plays a key role in many biological processes including pattern formation during early Drosophila embryogenesis. In this process, the anterior determinant Bicoid (BCD) acts not only as a transcriptional activator of segmentation genes but also causes specific translational repr ...
... Translational control plays a key role in many biological processes including pattern formation during early Drosophila embryogenesis. In this process, the anterior determinant Bicoid (BCD) acts not only as a transcriptional activator of segmentation genes but also causes specific translational repr ...