Osmoadaptation mechanisms in prokaryotes
... miotherma, a thermophilic bacterium of the order Thermotogales, accumulates α-glutamate at the highest salinity tolerated but accumulates only small amounts of this amino acid at low salinities [17]. On the other hand, the closely related species P. mobilis accumulates not only α-glutamate but also ...
... miotherma, a thermophilic bacterium of the order Thermotogales, accumulates α-glutamate at the highest salinity tolerated but accumulates only small amounts of this amino acid at low salinities [17]. On the other hand, the closely related species P. mobilis accumulates not only α-glutamate but also ...
... The DH% obtained was higher with protease XIV using collagen from fins (28.1±2.2) and arms (22.3±3.3), compared with hydrolysates obtained with trypsin using collagen from fins (24±2.8) and arms (15.4±2.2). Although the bioactivity of proteins may be increased by hydrolysis with specific enzymes, pr ...
High pKa variability of cysteine residues in structural databases and
... these oxidations are effectively counteracted through natural defensive mechanisms [9]; however, intense and/or persistent levels of ROS stress ultimately lead to protein damage (misfolding, cross-linkages, inactivation of functional residues) [10,11]. A critically important aspect of Cys reactivity ...
... these oxidations are effectively counteracted through natural defensive mechanisms [9]; however, intense and/or persistent levels of ROS stress ultimately lead to protein damage (misfolding, cross-linkages, inactivation of functional residues) [10,11]. A critically important aspect of Cys reactivity ...
Unit 8 Acids and Bases Notes (answers)
... - polyprotic acids dissociate one proton at a time. Each successive proton donation has its own Ka, which gets smaller until the last proton is donated. (Ka1 > Ka2 > Ka3 > …). - the intermediates (conjugate bases of each dissociation except the last one) are themselves acids. Thus, diprotic acid can ...
... - polyprotic acids dissociate one proton at a time. Each successive proton donation has its own Ka, which gets smaller until the last proton is donated. (Ka1 > Ka2 > Ka3 > …). - the intermediates (conjugate bases of each dissociation except the last one) are themselves acids. Thus, diprotic acid can ...
topological changes in the cyp3a4 active site probed with
... Downloaded from dmd.aspetjournals.org at ASPET Journals on June 14, 2017 ...
... Downloaded from dmd.aspetjournals.org at ASPET Journals on June 14, 2017 ...
Detection and Estimation of Collagen
... single method could combine accuracy with freedom from errors caused by interferring substances. Consequently, several different experimental approaches can be found in the literature, depending upon the nature of the test material. Chloramine T is now generally preferred for use as an oxidant in th ...
... single method could combine accuracy with freedom from errors caused by interferring substances. Consequently, several different experimental approaches can be found in the literature, depending upon the nature of the test material. Chloramine T is now generally preferred for use as an oxidant in th ...
Unusual properties of the fungal conventional kinesin neck domain
... However, the elution volume relative to the standard proteins was unchanged, demonstrating that NcKin378 remains monomeric under low salt conditions. It was not possible to test even lower ionic strengths because of interactions between the Sephadex matrix and the sample. The slightly longer NcKin38 ...
... However, the elution volume relative to the standard proteins was unchanged, demonstrating that NcKin378 remains monomeric under low salt conditions. It was not possible to test even lower ionic strengths because of interactions between the Sephadex matrix and the sample. The slightly longer NcKin38 ...
Stimulation of glycolysis in anaerobic elongation of pondweed
... 10±6 m3 of 80% ethanol for 30 min. The combined extract was dried under reduced pressure and redissoloved in deionized water. The ethanol-insoluble materials were used for analysis of polysaccharides. The water-soluble substances were passed through an ionexchange column packed with Dowex 50W-X8 (NH ...
... 10±6 m3 of 80% ethanol for 30 min. The combined extract was dried under reduced pressure and redissoloved in deionized water. The ethanol-insoluble materials were used for analysis of polysaccharides. The water-soluble substances were passed through an ionexchange column packed with Dowex 50W-X8 (NH ...
SURVEY AND SUMMARY A profusion of upstream open reading
... in plants. The presence of the two adjacent nearly universally conserved prolines is significant because of the known effects of this amino acid on translation termination when encoded by the penultimate or last sense codon of an ORF (25). Within each of the four orthologous uCC groups there are addit ...
... in plants. The presence of the two adjacent nearly universally conserved prolines is significant because of the known effects of this amino acid on translation termination when encoded by the penultimate or last sense codon of an ORF (25). Within each of the four orthologous uCC groups there are addit ...
URIC ACID
... - Levels initially fall by up to 25% in the first trimester, but then rise to values 20% higher than in the non-pregnant state. - Commonly used reference values: - Adult females: 0.21-0.36 mmol/l - Adult males: 0.31-0.47 mmol/l. B. Urinary urate: ...
... - Levels initially fall by up to 25% in the first trimester, but then rise to values 20% higher than in the non-pregnant state. - Commonly used reference values: - Adult females: 0.21-0.36 mmol/l - Adult males: 0.31-0.47 mmol/l. B. Urinary urate: ...
The Regulation of Acetyl Coenzyme A Synthesis in Chloroplasts
... Pyruvate Dehydrogenase-Complex, Acetyl-CoA Synthetase, Chloroplasts The enzymatic activities of the pyruvate dehydrogenase complex (PDC) and acetyl-CoA synthe tase (ACS) have been compared in extracts of plastids isolated from spinach leaves and from both green and etiolated pea seedlings. A ll pla ...
... Pyruvate Dehydrogenase-Complex, Acetyl-CoA Synthetase, Chloroplasts The enzymatic activities of the pyruvate dehydrogenase complex (PDC) and acetyl-CoA synthe tase (ACS) have been compared in extracts of plastids isolated from spinach leaves and from both green and etiolated pea seedlings. A ll pla ...
CHM 303 - Unaab.edu.ng
... choice. Margarine made from corn, safflower, or sunflower oils is much lower in saturated fatty acids than is butter, which is made from milk fat. However, margarine may present its own health risks. Its fatty acids contain trans-double bonds (introduced by the hydrogenation process), which may also ...
... choice. Margarine made from corn, safflower, or sunflower oils is much lower in saturated fatty acids than is butter, which is made from milk fat. However, margarine may present its own health risks. Its fatty acids contain trans-double bonds (introduced by the hydrogenation process), which may also ...
The Three-Dimensional Structure of Aspergillus niger Pectin Lyase
... from and cover the parallel b helix core. The differences have been exploited in the present research to improve the ...
... from and cover the parallel b helix core. The differences have been exploited in the present research to improve the ...
The Intermediary Metabolism of Cholesterol
... nutritional states excepting those which involve excessive feeding of cholesterol itself. Cholesterol is not required as an essential dietary constituent since synthetic, sterol-free diets are adequate for growth and maintenance of most animals. In this respect the larvae of certain insects' and som ...
... nutritional states excepting those which involve excessive feeding of cholesterol itself. Cholesterol is not required as an essential dietary constituent since synthetic, sterol-free diets are adequate for growth and maintenance of most animals. In this respect the larvae of certain insects' and som ...
Structure and Function of Mammalian Ribosomes
... intact. This then allows us to correlate function more quantitatively with structure. We wanted to minimize the harshness of the conditions required for subunit dissociation. Therefore, we prepared subunits from free 80 s ribosomes rather than from polysomes. To accomplish this we prepared pure poly ...
... intact. This then allows us to correlate function more quantitatively with structure. We wanted to minimize the harshness of the conditions required for subunit dissociation. Therefore, we prepared subunits from free 80 s ribosomes rather than from polysomes. To accomplish this we prepared pure poly ...
Fibrous Proteins
... Collagen is formed from tropocollagen subunits. The triple helix in tropocollagen is highly extended and strong. ...
... Collagen is formed from tropocollagen subunits. The triple helix in tropocollagen is highly extended and strong. ...
Synthesis and Structural Studies of Calcium and Magnesium
... This enormous research project would not have been possible without the grace of God and able leadership and support of two distinguished professors: Dr. Karin Ruhlandt and Dr. Jon Zubieta of the Chemistry Department of Syracuse University, who nurtured the seed for the bone project. I am especially ...
... This enormous research project would not have been possible without the grace of God and able leadership and support of two distinguished professors: Dr. Karin Ruhlandt and Dr. Jon Zubieta of the Chemistry Department of Syracuse University, who nurtured the seed for the bone project. I am especially ...
Sensitive Detection of Botulinum Neurotoxin Type A
... the antibody. Generation of cleaved peptide was monitored using reverse phase HPLC with a fluorescence detector. Summary of Results: Initial studies indicated that as low as 1 pg of BoNT/A can be captured and detected using SNAPtide® in an overnight digestion at room temperature. Optimization of thi ...
... the antibody. Generation of cleaved peptide was monitored using reverse phase HPLC with a fluorescence detector. Summary of Results: Initial studies indicated that as low as 1 pg of BoNT/A can be captured and detected using SNAPtide® in an overnight digestion at room temperature. Optimization of thi ...
Identification of Modified Amino Acids by Edman Sequencing
... Fmoc amino acid and the HATU coupling reagent, except in the cases of Fmoc-3Me-His-OH and Fmoc Lys(Me3)-OH, which were available in smaller amounts. In these cases, PyAOP (0.7 mmole) was used as the coupling reagent. Three amino acids, Fmoc-Lys(Me2)-OH, Fmoc Lys(Me3)-OH, and Fmoc-3-Me-His-OH, were i ...
... Fmoc amino acid and the HATU coupling reagent, except in the cases of Fmoc-3Me-His-OH and Fmoc Lys(Me3)-OH, which were available in smaller amounts. In these cases, PyAOP (0.7 mmole) was used as the coupling reagent. Three amino acids, Fmoc-Lys(Me2)-OH, Fmoc Lys(Me3)-OH, and Fmoc-3-Me-His-OH, were i ...
Relació estructura-funció en la família de transportadors d'aminoàcids heteromultimèrics.
... related to neurotransmission (the GABA transporter GAT1 and the glutamate transporter GLT-1), these data allowed microsequencing or generation of specific antibodies that have been used to isolate cDNA clones (184, 424). Alternative strategies and serendipity allowed the identification of up to 22 c ...
... related to neurotransmission (the GABA transporter GAT1 and the glutamate transporter GLT-1), these data allowed microsequencing or generation of specific antibodies that have been used to isolate cDNA clones (184, 424). Alternative strategies and serendipity allowed the identification of up to 22 c ...
Characteristics of Phenylacrylic Acid Decarboxylase
... living systems. Due to the potential for decarboxylases to produce optically pure products under mild reaction conditions, we have sought to further investigate phenylacrylic acid decarboxylase 1 (PAD1) and ferulic acid decarboxylase (FDC) from ...
... living systems. Due to the potential for decarboxylases to produce optically pure products under mild reaction conditions, we have sought to further investigate phenylacrylic acid decarboxylase 1 (PAD1) and ferulic acid decarboxylase (FDC) from ...