Isolating, Cloning, and Sequencing DNA
... These technical breakthroughs in genetic engineering the ability to manipulate DNA with precision in a test tube or an organism have had a dramatic impact on all aspects of cell biology by facilitating the study of cells and their macromolecules in previously unimagined ways. They have led to the di ...
... These technical breakthroughs in genetic engineering the ability to manipulate DNA with precision in a test tube or an organism have had a dramatic impact on all aspects of cell biology by facilitating the study of cells and their macromolecules in previously unimagined ways. They have led to the di ...
Guidelines for separating DNA (Deoxyribonucleic Acid) using gel
... molecular biology to separate macromolecules such as proteins and nucleic acids, (of which DNA is an example) based on physical properties such as their size, shape and electric charge. In molecular biology, gel electrophoresis is one of the standard, analytical, biochemical tools used to study gene ...
... molecular biology to separate macromolecules such as proteins and nucleic acids, (of which DNA is an example) based on physical properties such as their size, shape and electric charge. In molecular biology, gel electrophoresis is one of the standard, analytical, biochemical tools used to study gene ...
Looking at long molecules in solution: what happens when they are
... Many molecules in biological systems are long (e.g. DNA) or part of long assemblies of molecules (e.g. fibrous proteins or membrane-bound molecules). While it is clear that the structures of such moieties are important to their function, many of the powerful techniques of structural biology, includin ...
... Many molecules in biological systems are long (e.g. DNA) or part of long assemblies of molecules (e.g. fibrous proteins or membrane-bound molecules). While it is clear that the structures of such moieties are important to their function, many of the powerful techniques of structural biology, includin ...
III-D-2a
... with whole plants. III-D-5-b. BL3-P (NOTE: BLP-3 WORK IS NOT TO BE CONDUCTD AT VCU) or BL2-P + biological containment is recommended for experiments involving plants containing cloned genomes of readily transmissible exotic infectious agents with recognized potential for serious detrimental effects ...
... with whole plants. III-D-5-b. BL3-P (NOTE: BLP-3 WORK IS NOT TO BE CONDUCTD AT VCU) or BL2-P + biological containment is recommended for experiments involving plants containing cloned genomes of readily transmissible exotic infectious agents with recognized potential for serious detrimental effects ...
Restriction mapping
... modification system. Today, most commercially available REs are not purified from their natural sources.. Instead, REs are usually isolated from bacteria that overexpress large quantities of REs from plasmids. These recombinant REs have often been engineered by molecular biologists to include amino ...
... modification system. Today, most commercially available REs are not purified from their natural sources.. Instead, REs are usually isolated from bacteria that overexpress large quantities of REs from plasmids. These recombinant REs have often been engineered by molecular biologists to include amino ...
LNA-PNA Comparison4
... over 70°C. In these triplexes, one PNA strand hybridizes to DNA through standard Watson–Crick base pairing rules, while the other PNA strand binds to DNA through Hoogsteen hydrogen bonds. The resulting structure is called P-loops. The stability of these triple helixes is so high that homopyrimidine ...
... over 70°C. In these triplexes, one PNA strand hybridizes to DNA through standard Watson–Crick base pairing rules, while the other PNA strand binds to DNA through Hoogsteen hydrogen bonds. The resulting structure is called P-loops. The stability of these triple helixes is so high that homopyrimidine ...
NucleoSpin 96 Flash Plasmid and Large-Construct DNA
... host cells by SDS / alkaline lysis after resuspension of the pelleted bacteria (buffers F2 and F1, respectively). The resulting lysate is then neutralized by addition of Buffer F3 – cell debris will precipitate together with SDS. Incubation in boiling water (optional) inactivates nucleases which are ...
... host cells by SDS / alkaline lysis after resuspension of the pelleted bacteria (buffers F2 and F1, respectively). The resulting lysate is then neutralized by addition of Buffer F3 – cell debris will precipitate together with SDS. Incubation in boiling water (optional) inactivates nucleases which are ...
The polymerase chain reaction
... different DNA sequences. By targeting multiple genes at once, additional information may be gained from a single test-run that otherwise would require several times the reagents and more time to perform. Annealing temperatures for each of the primer sets must be optimized to work correctly within a ...
... different DNA sequences. By targeting multiple genes at once, additional information may be gained from a single test-run that otherwise would require several times the reagents and more time to perform. Annealing temperatures for each of the primer sets must be optimized to work correctly within a ...
96-well PCR Cleanup Manual for Non
... After DNA is captured onto a filter media, impurities are washed away and the purified PCR product is eluted into an isotonic buffer. Eluted DNA is free of polymerases and other protein adjuncts (>99.5% removal), as well as nucleotides, salts and primers (see figure 1). The highly purified product i ...
... After DNA is captured onto a filter media, impurities are washed away and the purified PCR product is eluted into an isotonic buffer. Eluted DNA is free of polymerases and other protein adjuncts (>99.5% removal), as well as nucleotides, salts and primers (see figure 1). The highly purified product i ...
JOIN2004 Universidade do Minho
... A restriction enzyme is a protein that cuts DNA at short, specific sequences EcoRI cuts at the restriction site GAATTC, between the G and A, on both complementary strands (the reverse complement of GAATTC is GAATTC), leaving "sticky ends" for insertion into a vector for cloning and sequencing. A res ...
... A restriction enzyme is a protein that cuts DNA at short, specific sequences EcoRI cuts at the restriction site GAATTC, between the G and A, on both complementary strands (the reverse complement of GAATTC is GAATTC), leaving "sticky ends" for insertion into a vector for cloning and sequencing. A res ...
Maurice Wilkins
Maurice Hugh Frederick Wilkins CBE FRS (15 December 1916 – 5 October 2004) was a New Zealand-born English physicist and molecular biologist, and Nobel Laureate whose research contributed to the scientific understanding of phosphorescence, isotope separation, optical microscopy and X-ray diffraction, and to the development of radar. He is best known for his work at King's College, London on the structure of DNA which falls into three distinct phases. The first was in 1948–50 where his initial studies produced the first clear X-ray images of DNA which he presented at a conference in Naples in 1951 attended by James Watson. During the second phase of work (1951–52) he produced clear ""B form"" ""X"" shaped images from squid sperm which he sent to James Watson and Francis Crick causing Watson to write ""Wilkins... has obtained extremely excellent X-ray diffraction photographs""[of DNA]. Throughout this period Wilkins was consistent in his belief that DNA was helical even when Rosalind Franklin expressed strong views to the contrary.In 1953 Franklin instructed Raymond Gosling to give Wilkins, without condition, a high quality image of ""B"" form DNA which she had unexpectedly produced months earlier but had “put it aside” to concentrate on other work. Wilkins, having checked that he was free to personally use the photograph to confirm his earlier results, showed it to Watson without the consent of Rosalind Franklin. This image, along with the knowledge that Linus Pauling had published an incorrect structure of DNA, “mobilised” Watson to restart model building efforts with Crick. Important contributions and data from Wilkins, Franklin (obtained via Max Perutz) and colleagues in Cambridge enabled Watson and Crick to propose a double-helix model for DNA. The third and longest phase of Wilkins' work on DNA took place from 1953 onwards. Here Wilkins led a major project at King's College, London, to test, verify and make significant corrections to the DNA model proposed by Watson and Crick and to study the structure of RNA. Wilkins, Crick and Watson were awarded the 1962 Nobel Prize for Physiology or Medicine, ""for their discoveries concerning the molecular structure of nucleic acids and its significance for information transfer in living material.""