Specific PCR Primers for the Identification of Salmonella enterica
... Salmonella is one of the most common pathogens and a major cause of foodborne diseases in human worldwide (1,2,3). Contaminated poultry products have been identified as the principal sources of Salmonella leading to foodborne illness in human(4.5). The most common serotypes of Salmonella isolated fr ...
... Salmonella is one of the most common pathogens and a major cause of foodborne diseases in human worldwide (1,2,3). Contaminated poultry products have been identified as the principal sources of Salmonella leading to foodborne illness in human(4.5). The most common serotypes of Salmonella isolated fr ...
Forensic analysis of expirated blood
... trauma and/or machinery accidents) is often important but difficult to determine due to ...
... trauma and/or machinery accidents) is often important but difficult to determine due to ...
Maintenance of genomic integrity by p53: complementary
... are far less clear. Furthermore, it is still not known, whether and how p53 directly participates in DNA repair processes, despite some evidence pointing to this possibility. Last but not least, a possible role of p53 in the control of genomic integrity in its non-induced state, i.e., in the absence ...
... are far less clear. Furthermore, it is still not known, whether and how p53 directly participates in DNA repair processes, despite some evidence pointing to this possibility. Last but not least, a possible role of p53 in the control of genomic integrity in its non-induced state, i.e., in the absence ...
f o r e n s i c science - and
... profile. Generally, all these pieces of evidence do not contain a substantial amount of biological material and are processed for DNA without going through any type of serological screening to maximize the amount of sample available for DNA testing. Cases involving kinship determination do not requi ...
... profile. Generally, all these pieces of evidence do not contain a substantial amount of biological material and are processed for DNA without going through any type of serological screening to maximize the amount of sample available for DNA testing. Cases involving kinship determination do not requi ...
Formosa haliotis sp. nov., a brown-alga
... DNA–DNA hybridizations were performed between isolates LMG 28520T and LMG 28522 and the type strains of their nearest phylogenetic neighbours (F. algae LMG 28216T and F. arctica LMG 28318T). Hybridizations were performed under stringent conditions in a solution containing 50 % (v/v) formamide at 35 ...
... DNA–DNA hybridizations were performed between isolates LMG 28520T and LMG 28522 and the type strains of their nearest phylogenetic neighbours (F. algae LMG 28216T and F. arctica LMG 28318T). Hybridizations were performed under stringent conditions in a solution containing 50 % (v/v) formamide at 35 ...
Quantitative Analysis of the Kinetics of End
... that affect RecA ®lament assembly and disassembly. Filament assembly occurs in at least two major phases. Nucleation is generally rate limiting on all DNAs and under all conditions, and is followed by a rapid and unidirectional extension of the ®lament until the available RecA protein and/or contigu ...
... that affect RecA ®lament assembly and disassembly. Filament assembly occurs in at least two major phases. Nucleation is generally rate limiting on all DNAs and under all conditions, and is followed by a rapid and unidirectional extension of the ®lament until the available RecA protein and/or contigu ...
Etude du régime alimentaire des carnivores par des techniques
... aim of my study was to develop a DNA-based universal method to study carnivores diet using environmental samples, and to apply this approach to several carnivore species. The main focus was on the diet of endangered and elusive wild felid species of Asia. Studying carnivores diet by characterizing t ...
... aim of my study was to develop a DNA-based universal method to study carnivores diet using environmental samples, and to apply this approach to several carnivore species. The main focus was on the diet of endangered and elusive wild felid species of Asia. Studying carnivores diet by characterizing t ...
1 - IPPC
... indicated in Figures 1 and 2 are the minimum requirements for the diagnosis, but further tests may be required by the national plant protection organization (NPPO), especially for the first report in a country. For example, serological tests may facilitate a presumptive diagnosis of symptomatic plan ...
... indicated in Figures 1 and 2 are the minimum requirements for the diagnosis, but further tests may be required by the national plant protection organization (NPPO), especially for the first report in a country. For example, serological tests may facilitate a presumptive diagnosis of symptomatic plan ...
One-stop polymerase chain reaction (PCR): An improved PCR
... (v/v). 5×compatible dye mix included 100g Ficoll400/L, 0.1 g cresol red/L, 0.8 g tartrazine/L, Gelred 1.25× (Original concentration is 10000× in DMSO; Biocompare, USA). 2×One-stop PCR master mix (2×Red mix) was prepared as follows: 10×PCR reaction buffer, 2 µl; 40 mM dNTP (10mM each), 0.5 µl; Taq DN ...
... (v/v). 5×compatible dye mix included 100g Ficoll400/L, 0.1 g cresol red/L, 0.8 g tartrazine/L, Gelred 1.25× (Original concentration is 10000× in DMSO; Biocompare, USA). 2×One-stop PCR master mix (2×Red mix) was prepared as follows: 10×PCR reaction buffer, 2 µl; 40 mM dNTP (10mM each), 0.5 µl; Taq DN ...
Phylogenomics of type II DNA topoisomerases
... Archaea, B for Bacteria, E for Eukarya and V for Virus. Letters in brackets indicates that the corresponding Topo II has been transferred in this domain from another one. Activities: rel, relaxation; sup, supercoiling; , negative superturns; þ, positive superturns. Representative species are those ...
... Archaea, B for Bacteria, E for Eukarya and V for Virus. Letters in brackets indicates that the corresponding Topo II has been transferred in this domain from another one. Activities: rel, relaxation; sup, supercoiling; , negative superturns; þ, positive superturns. Representative species are those ...
Biotechnology Explorer™ GMO Investigator™ Kit: A - Bio-Rad
... mixed (multiplexed) together but are detected simultaneously, either as bands on an agarose gel or as a SYBR Green I dye signal in this real-time extension. In the description above, primer pairs also include a matched fluorescent probe for each primer set, which allows multiple PCR reactions to be ...
... mixed (multiplexed) together but are detected simultaneously, either as bands on an agarose gel or as a SYBR Green I dye signal in this real-time extension. In the description above, primer pairs also include a matched fluorescent probe for each primer set, which allows multiple PCR reactions to be ...
Supplementary Materials and methods (doc 154K)
... detect small differences in fitness. The exact initial proportions were confirmed via flow cytometry (see conditions below). Mixtures were diluted 200-fold in fresh LB and competed for 16 hours at 37°C with no agitation (~7 generations). Again, the final proportion was measured by flow cytometry. Th ...
... detect small differences in fitness. The exact initial proportions were confirmed via flow cytometry (see conditions below). Mixtures were diluted 200-fold in fresh LB and competed for 16 hours at 37°C with no agitation (~7 generations). Again, the final proportion was measured by flow cytometry. Th ...
Introduction to Forensic Sciences - Beck-Shop
... a suspicious fluid or stain is saliva, semen or blood. Tests are available to determine if the evidence, such as blood, is of human origin. Once it is identified as human, then DNA (deoxyribonucleic acid) testing can be attempted. Forensic DNA testing is sometimes referred to as “DNA fingerprinting. ...
... a suspicious fluid or stain is saliva, semen or blood. Tests are available to determine if the evidence, such as blood, is of human origin. Once it is identified as human, then DNA (deoxyribonucleic acid) testing can be attempted. Forensic DNA testing is sometimes referred to as “DNA fingerprinting. ...
Taxonomic characterization of Ochrobactrum sp. isolates from soil
... from isolate 1a, SCII24T, OgA9aT, OiC8a, OiC8-6, LMG 5140, CLM14 and CLM18 was amplified by PCR using primers rD1 and fD1 (Weisburg et al., 1991). Amplificates of isolate 1a, SCII24T, OgA9aT, OiC8a and OiC8-6 were purified by low-melting agarose gel electrophoresis and sequenced following the dideox ...
... from isolate 1a, SCII24T, OgA9aT, OiC8a, OiC8-6, LMG 5140, CLM14 and CLM18 was amplified by PCR using primers rD1 and fD1 (Weisburg et al., 1991). Amplificates of isolate 1a, SCII24T, OgA9aT, OiC8a and OiC8-6 were purified by low-melting agarose gel electrophoresis and sequenced following the dideox ...
Rh phenotype prediction by DNA typing and its
... the most prevalent RHD-negative allele cannot yet be detected specifically. For the time being, we prefer therefore the term DNA typing rather than genotyping, when an RhD phenotype prediction by molecular techniques is attempted. We have summarized the molecular genetics of the RH genes according t ...
... the most prevalent RHD-negative allele cannot yet be detected specifically. For the time being, we prefer therefore the term DNA typing rather than genotyping, when an RhD phenotype prediction by molecular techniques is attempted. We have summarized the molecular genetics of the RH genes according t ...
DNA METHODS FOR HLA TYPING A WORKBOOK FOR - ASHI-U
... clotting (platelets). Certain types of cancer affect these stem cells and can be fatal to the patient. One therapy for the treatment of these blood diseases like leukemia and aplastic anemia is bone marrow (or hematopoietic stem cell) transplantation. In this procedure, the abnormal bone marrow of t ...
... clotting (platelets). Certain types of cancer affect these stem cells and can be fatal to the patient. One therapy for the treatment of these blood diseases like leukemia and aplastic anemia is bone marrow (or hematopoietic stem cell) transplantation. In this procedure, the abnormal bone marrow of t ...
KingFisher Pure DNA Plant Kit
... designed plastic consumables, and the easy-to-use BindIt Software 3.2. The KingFisher Pure DNA Plant Kit is optimized and ready for use with the KingFisher Flex or KingFisher Duo. KingFisher magnetic particle processors are intended for professional research use by trained personnel. Detailed inform ...
... designed plastic consumables, and the easy-to-use BindIt Software 3.2. The KingFisher Pure DNA Plant Kit is optimized and ready for use with the KingFisher Flex or KingFisher Duo. KingFisher magnetic particle processors are intended for professional research use by trained personnel. Detailed inform ...
Molecular Identification of Nematodes Manual
... a brand new tube of Taq, or it is almost empty and you want to get the last bit of it, then blip the Taq tube as well.) *To “blip” a tube of solution is to spin it in a microcentrifuge for only a second. (If you are spinning 0.5ml PCR tubes, make sure they are properly stabilized in the rotor. They ...
... a brand new tube of Taq, or it is almost empty and you want to get the last bit of it, then blip the Taq tube as well.) *To “blip” a tube of solution is to spin it in a microcentrifuge for only a second. (If you are spinning 0.5ml PCR tubes, make sure they are properly stabilized in the rotor. They ...
Document
... • Adenine must pair with Thymine • Guanine must pair with Cytosine • Their amounts in a given DNA molecule will be about the same. ...
... • Adenine must pair with Thymine • Guanine must pair with Cytosine • Their amounts in a given DNA molecule will be about the same. ...
Gel immobilization of acrylamide-modified single
... ing methods. It has been widely used in applications of SNP analysis [5], clone checking [6], identification of short DNA sequences used in bacterial typing [7], and recently in highthroughput genome sequencing [8]. Although the pyrosequencing chemistry itself is quite simple and straightforward, te ...
... ing methods. It has been widely used in applications of SNP analysis [5], clone checking [6], identification of short DNA sequences used in bacterial typing [7], and recently in highthroughput genome sequencing [8]. Although the pyrosequencing chemistry itself is quite simple and straightforward, te ...
Mutagenesis Point mutations Deletions Insertions Types of
... Cloning. Gibson Assembly eliminates the need to engineer restriction enzyme cut sites within DNA when assembling fragments together. DNA molecules are designed such that neighboring fragments contain a 20-40 bp overlapping sequence. If the DNA fragments originate from PCR products, the overlapping s ...
... Cloning. Gibson Assembly eliminates the need to engineer restriction enzyme cut sites within DNA when assembling fragments together. DNA molecules are designed such that neighboring fragments contain a 20-40 bp overlapping sequence. If the DNA fragments originate from PCR products, the overlapping s ...
Analysis of Cross Sequence Similarities for Multiple - PolyU
... sample sequence while the subsequence ‘ACGCAT’ listed between 7 and 12 is the 7th to 12th bases of the sample sequence. The vertical line located between two bases indicates that the upper base is identical to the lower base. By comparing the first subsequence (1st to 6th bases) and the second subse ...
... sample sequence while the subsequence ‘ACGCAT’ listed between 7 and 12 is the 7th to 12th bases of the sample sequence. The vertical line located between two bases indicates that the upper base is identical to the lower base. By comparing the first subsequence (1st to 6th bases) and the second subse ...
Resolving Individuals Contributing Trace Amounts of DNA to Highly
... components of the mixture [8]. Nevertheless, these methods based on STRs expectedly suffer from limited power when using severely degraded DNA [8,9]. Mitochondrial DNA (mtDNA) based on hypervariable region sequencing is useful when analyzing degraded DNA due to its high copy number and improved stab ...
... components of the mixture [8]. Nevertheless, these methods based on STRs expectedly suffer from limited power when using severely degraded DNA [8,9]. Mitochondrial DNA (mtDNA) based on hypervariable region sequencing is useful when analyzing degraded DNA due to its high copy number and improved stab ...
NUCLEIC ACID ECONOMY IN BACTERIA INFECTED WITH
... survival of 10-~) in chilled centrifuge tubes. After about 30 minutes in an ice bath, the tubes were warmed 30 minutes at 37°C. and refrigerated overnight. The next morning I0 #g. desoxyribonuclease was added to each tube and the contents were diluted to 10 ml. with 0.1 per cent peptone water. After ...
... survival of 10-~) in chilled centrifuge tubes. After about 30 minutes in an ice bath, the tubes were warmed 30 minutes at 37°C. and refrigerated overnight. The next morning I0 #g. desoxyribonuclease was added to each tube and the contents were diluted to 10 ml. with 0.1 per cent peptone water. After ...
DNA profiling
DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is a forensic technique used to identify individuals by characteristics of their DNA. A DNA profile is a small set of DNA variations that is very likely to be different in all unrelated individuals, thereby being as unique to individuals as are fingerprints (hence the alternate name for the technique). DNA profiling should not be confused with full genome sequencing. First developed and used in 1985, DNA profiling is used in, for example, parentage testing and criminal investigation, to identify a person or to place a person at a crime scene, techniques which are now employed globally in forensic science to facilitate police detective work and help clarify paternity and immigration disputes.Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic (""identical"") twins. DNA profiling uses repetitive (""repeat"") sequences that are highly variable, called variable number tandem repeats (VNTRs), in particular short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but are so variable that unrelated individuals are extremely unlikely to have the same VNTRs.The DNA profiling technique nowadays used is based on technology developed in 1988.