Noninvasive prenatal diagnosis of fetal chromosomal aneuploidies
... fetal chromosomal aneuploidy, using trisomy 18 as a model system (see later sections) (20 ). Since the development of the SERPINB5 marker, many other fetal epigenetic markers suitable for detection in maternal plasma have been described, including the RASSF1A gene on chromosome 3 (21, 22 ) and numer ...
... fetal chromosomal aneuploidy, using trisomy 18 as a model system (see later sections) (20 ). Since the development of the SERPINB5 marker, many other fetal epigenetic markers suitable for detection in maternal plasma have been described, including the RASSF1A gene on chromosome 3 (21, 22 ) and numer ...
Original Article:
... biased the conclusions. However, for coagulase negative staphylococci (CoNS), infections caused by several different strains are often considered (ref). Up to date, the lack of sufficient tools to characterize a multiclonal community of S. aureus may have hampered proper treatment. ...
... biased the conclusions. However, for coagulase negative staphylococci (CoNS), infections caused by several different strains are often considered (ref). Up to date, the lack of sufficient tools to characterize a multiclonal community of S. aureus may have hampered proper treatment. ...
Two DNA sites for MelR in the same orientation are sufficient for
... just two bound regulator molecules depends on both target sequences being in the same orientation (Carra & Schleif, 1993). MelR-dependent repression at the TB33 melR promoter is insensitive to spacing but reduced by a decoy DNA site for MelR ...
... just two bound regulator molecules depends on both target sequences being in the same orientation (Carra & Schleif, 1993). MelR-dependent repression at the TB33 melR promoter is insensitive to spacing but reduced by a decoy DNA site for MelR ...
Mutual Interactions of the Phosphate Groups in Locally Deformed
... (Borah et al. 1985; Nishimura et al. 1986; Sarma et al. 1986). It is also worth of being noticed that at 0.7 mol/l NaCl the backbones of both B- and A-DNA tend to overwind, presumably to counteract base stacking tendencies to unwind the double helices (Šponer and Kypr 1989). At high-salt, even the i ...
... (Borah et al. 1985; Nishimura et al. 1986; Sarma et al. 1986). It is also worth of being noticed that at 0.7 mol/l NaCl the backbones of both B- and A-DNA tend to overwind, presumably to counteract base stacking tendencies to unwind the double helices (Šponer and Kypr 1989). At high-salt, even the i ...
PDF
... manipulation of paramagnetic beads using a gradient of magnetic field [14]. The two techniques have been developing head to head to achieve nowadays a remarkable level of sophistication. They have their own unique advantages but, largely, offer similar capabilities and require the same workflow. The ...
... manipulation of paramagnetic beads using a gradient of magnetic field [14]. The two techniques have been developing head to head to achieve nowadays a remarkable level of sophistication. They have their own unique advantages but, largely, offer similar capabilities and require the same workflow. The ...
Cellular DNA Polymerases - DNA Replication and Human Disease
... localized in the mitochondria. Therefore, pol-y is thought to be the enzyme that replicates the mitochondria1 DNA. The general enzymatic properties and characteristics that distinguish each DNA pol are summarized in Table 1, and the optimal assay conditions for each enzyme, such as pH, preferred pri ...
... localized in the mitochondria. Therefore, pol-y is thought to be the enzyme that replicates the mitochondria1 DNA. The general enzymatic properties and characteristics that distinguish each DNA pol are summarized in Table 1, and the optimal assay conditions for each enzyme, such as pH, preferred pri ...
MOLECULAR STRUCTURE OF DNA AND RNA
... proteins and DNA assemble to make new viruses that are subsequently released from the cell by lysis (i.e., cell breakage). To verify that DNA is the genetic material of T2, Hershey and Chase devised a method to separate the phage coat, which is attached to the outside of the bacterium, from the gene ...
... proteins and DNA assemble to make new viruses that are subsequently released from the cell by lysis (i.e., cell breakage). To verify that DNA is the genetic material of T2, Hershey and Chase devised a method to separate the phage coat, which is attached to the outside of the bacterium, from the gene ...
Detection of Agrobacterium vitis by polymerase chain reaction in
... tion and ethanol precipitation. Finally DNA was dissolved in 200 ml sterile distilled water and stored in aliquots at -75 °C. For PCR analysis 1 ml DNA was used. Since the routine use of PCR requires simple rapid protocols to obtain template DNA from a large number of samples we compared three direc ...
... tion and ethanol precipitation. Finally DNA was dissolved in 200 ml sterile distilled water and stored in aliquots at -75 °C. For PCR analysis 1 ml DNA was used. Since the routine use of PCR requires simple rapid protocols to obtain template DNA from a large number of samples we compared three direc ...
Sequence and Structural Selectivity of Nucleic Acid Binding Ligands†
... ethidium will be described in detail, to introduce the method, the presentation of data, and data interpretation. Competition dialysis results for ethidium are shown (Figure 2, left) as a bar graph in which the amount of ethidium bound is shown for each DNA structure included in the assay. These res ...
... ethidium will be described in detail, to introduce the method, the presentation of data, and data interpretation. Competition dialysis results for ethidium are shown (Figure 2, left) as a bar graph in which the amount of ethidium bound is shown for each DNA structure included in the assay. These res ...
lecture 1 File
... when haploid cells of opposite mating types MATa and MATα come into contact. Ruderfer et al. pointed out that, in nature, such contacts are frequent between closely related yeast cells for two reasons. The first is that cells of opposite mating type are present together in the same acus, the sac tha ...
... when haploid cells of opposite mating types MATa and MATα come into contact. Ruderfer et al. pointed out that, in nature, such contacts are frequent between closely related yeast cells for two reasons. The first is that cells of opposite mating type are present together in the same acus, the sac tha ...
NUCLEOTIDES AND NUCLEIC ACIDS
... atoms of the nitrogenous bases. The base of a nucleotide is joined covalently (at N-1 of pyrimidines and N-9 of purines) in an N--glycosyl bond to the 1 carbon of the pentose, and the phosphate is esterified to the 5 carbon. The N--glycosyl bond is formed by removal of the elements of water (a h ...
... atoms of the nitrogenous bases. The base of a nucleotide is joined covalently (at N-1 of pyrimidines and N-9 of purines) in an N--glycosyl bond to the 1 carbon of the pentose, and the phosphate is esterified to the 5 carbon. The N--glycosyl bond is formed by removal of the elements of water (a h ...
Chapter 6: DNA Replication and Telomere Maintenance I
... 4. On each human chromosome, it is estimated that there are between 10,000 and 100,000 origins of replication 5. Human origins of replication lack a consensus sequence, but are thought to be A-T rich (Have many A-T base pairs) B. Eukaryotic Linear DNA Synthesis: Unwinding the DNA At Origins of Repli ...
... 4. On each human chromosome, it is estimated that there are between 10,000 and 100,000 origins of replication 5. Human origins of replication lack a consensus sequence, but are thought to be A-T rich (Have many A-T base pairs) B. Eukaryotic Linear DNA Synthesis: Unwinding the DNA At Origins of Repli ...
A New DNA-Based S-Box
... walk. The objective of this test is to see the differences from the anticipated amount of visits to a variety of states in the random walk. This test is actually a series of eighteen tests. V. S-BOX TESTS CRITERIA There are a number of criteria that S-boxes must meet to be considered a good S-Box. - ...
... walk. The objective of this test is to see the differences from the anticipated amount of visits to a variety of states in the random walk. This test is actually a series of eighteen tests. V. S-BOX TESTS CRITERIA There are a number of criteria that S-boxes must meet to be considered a good S-Box. - ...
Cloning methods
... ends, and then purified by preparative gel electrophoresis, in order to remove uncut plasmid, because restriction digestion usually isn’t 100 % complete. The digested plasmid furthermore needs to be dephosphorylated, in order to prevent recircularization of plasmids without insert in the following l ...
... ends, and then purified by preparative gel electrophoresis, in order to remove uncut plasmid, because restriction digestion usually isn’t 100 % complete. The digested plasmid furthermore needs to be dephosphorylated, in order to prevent recircularization of plasmids without insert in the following l ...
Using the Wolbachia Bacterial Symbiont to Teach Inquiry
... UÊ Dissecting probe UÊ 3 Petri dishes UÊ Eyedropper or transfer pipette UÊ 95% ethanol UÊ 6 glass vials or similar containers UÊ 3 microcentrifuge tubes UÊ Sharpie marker, extra-fine point UÊ Labeling tape UÊ Digital camera or colored pencils UÊ Dissecting microscope (optional) UÊ Computer with Inte ...
... UÊ Dissecting probe UÊ 3 Petri dishes UÊ Eyedropper or transfer pipette UÊ 95% ethanol UÊ 6 glass vials or similar containers UÊ 3 microcentrifuge tubes UÊ Sharpie marker, extra-fine point UÊ Labeling tape UÊ Digital camera or colored pencils UÊ Dissecting microscope (optional) UÊ Computer with Inte ...
CORPORATE RESPONSIBILITY AT DNA CORPORATE
... DNA employees are active users of Yammer, their workplace social media application. A new Yammer group called “Ideajamit” (“Idea jams”) was launched in March 2016. The group is intended for all employees to share development ideas related to DNA’s products, services, internal operating methods or sy ...
... DNA employees are active users of Yammer, their workplace social media application. A new Yammer group called “Ideajamit” (“Idea jams”) was launched in March 2016. The group is intended for all employees to share development ideas related to DNA’s products, services, internal operating methods or sy ...
Restriction Enzymes in Microbiology, Biotechnology and
... in the presence of the same MTase. This is because modification is lost when the DNA replicates; it is an epigenetic, rather than a genetic, change. When a replication fork moves through a fully modified sequence, the two resulting daughter duplexes are ‘hemi-methylated’. Their parental DNA strands ...
... in the presence of the same MTase. This is because modification is lost when the DNA replicates; it is an epigenetic, rather than a genetic, change. When a replication fork moves through a fully modified sequence, the two resulting daughter duplexes are ‘hemi-methylated’. Their parental DNA strands ...
Commonly Used STR Markers
... 2. For D#S### markers – always use first published report of marker 3. First repeat is the first 5’ nucleotides that define repeat unit 4. Microvariants: ...
... 2. For D#S### markers – always use first published report of marker 3. First repeat is the first 5’ nucleotides that define repeat unit 4. Microvariants: ...
High-Throughput NGS Library Preparation
... related to sequence assembly and/or analysis, input DNA should ideally be fragmented to achieve a size distribution that approximates the range that will be targeted in any size selection procedures that you intend to apply. Any method of size selection will necessarily result in significant loss of ...
... related to sequence assembly and/or analysis, input DNA should ideally be fragmented to achieve a size distribution that approximates the range that will be targeted in any size selection procedures that you intend to apply. Any method of size selection will necessarily result in significant loss of ...
Genotyping BoLA-DRB3 alleles in Brazilian Dairy Gir cattle (Bos
... PCR-amplified product was purified using a GFXTM Purification Kit (Amersham Pharmacia Biotech, Piscataway, NJ). A volume of 2 µl of purified products containing approximately 200–500 ng of template DNA was cyclesequenced in a reaction total volume of 15 µl, containing 2 µl of ABI Prism Dye Terminato ...
... PCR-amplified product was purified using a GFXTM Purification Kit (Amersham Pharmacia Biotech, Piscataway, NJ). A volume of 2 µl of purified products containing approximately 200–500 ng of template DNA was cyclesequenced in a reaction total volume of 15 µl, containing 2 µl of ABI Prism Dye Terminato ...
bis-locked nucleic acids: a new tool for double helix invasion
... ensure the integrity of genetic information, the bases are on the inside the double helix whereas the phosphate and sugar groups are on the outside. Thus, most DNA information is not accessible until DNA is unwound prior to replication or transcription. In cells, enzymes called helicases achieve DNA ...
... ensure the integrity of genetic information, the bases are on the inside the double helix whereas the phosphate and sugar groups are on the outside. Thus, most DNA information is not accessible until DNA is unwound prior to replication or transcription. In cells, enzymes called helicases achieve DNA ...
Rolling circle transcription on smallest size double stranded DNA
... The base-pairing rules first described by Watson and Crick (Watson and Crick 1953) in their revolutionary Nature paper has since the early seventies been exploited in the creation of novel DNA combinations from naturally occurring sequences (Cohen, et al. 1973). The basis of this versatile method of ...
... The base-pairing rules first described by Watson and Crick (Watson and Crick 1953) in their revolutionary Nature paper has since the early seventies been exploited in the creation of novel DNA combinations from naturally occurring sequences (Cohen, et al. 1973). The basis of this versatile method of ...
Wildlife Crime - TRACE Wildlife Forensics Network
... technique and the quality of evidence gathered. DNA analysis of human blood and body fluids is now very sophisticated and can be performed on minute samples. Always consider whether the offender has cut himself, left behind cigarette ends or other sources of body fluid. DNA evidence is discussed fur ...
... technique and the quality of evidence gathered. DNA analysis of human blood and body fluids is now very sophisticated and can be performed on minute samples. Always consider whether the offender has cut himself, left behind cigarette ends or other sources of body fluid. DNA evidence is discussed fur ...
DNA profiling
DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is a forensic technique used to identify individuals by characteristics of their DNA. A DNA profile is a small set of DNA variations that is very likely to be different in all unrelated individuals, thereby being as unique to individuals as are fingerprints (hence the alternate name for the technique). DNA profiling should not be confused with full genome sequencing. First developed and used in 1985, DNA profiling is used in, for example, parentage testing and criminal investigation, to identify a person or to place a person at a crime scene, techniques which are now employed globally in forensic science to facilitate police detective work and help clarify paternity and immigration disputes.Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic (""identical"") twins. DNA profiling uses repetitive (""repeat"") sequences that are highly variable, called variable number tandem repeats (VNTRs), in particular short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but are so variable that unrelated individuals are extremely unlikely to have the same VNTRs.The DNA profiling technique nowadays used is based on technology developed in 1988.