Acetate kinase from CIostridiurn acetobutylicurn : a highly specific
... HE3B (Pharmacia) equilibrated with imidazole buffer. The column was washed with 40 ml of the same buffer, and the enzyme was eluted by an ATP gradient (0-1 mM ATP in imidazole buffer; 80 ml) at a maximal flow rate of 10 ml h-'. The fractions with high acetate kinase activity were pooled and shifted ...
... HE3B (Pharmacia) equilibrated with imidazole buffer. The column was washed with 40 ml of the same buffer, and the enzyme was eluted by an ATP gradient (0-1 mM ATP in imidazole buffer; 80 ml) at a maximal flow rate of 10 ml h-'. The fractions with high acetate kinase activity were pooled and shifted ...
53 - Lab Times
... be in danger of extinction when it comes to sequencing entire genomes like there’s no tomorrow. Instead of cloning single genes, as in the “old days”, many of today’s molecular biologists clone large sets of genes to determine their function. ...
... be in danger of extinction when it comes to sequencing entire genomes like there’s no tomorrow. Instead of cloning single genes, as in the “old days”, many of today’s molecular biologists clone large sets of genes to determine their function. ...
Case Study 5 Literature - Department of Chemistry
... purposes by supplying NADPH for anabolic reactions or in serine biosynthesis (10, 13, 14). Sequence analyses of the genes encoding GAPN of pea and maize, as well as of the bacterium Streptococcus mutans, indicated that GAPN enzymes are not related to phosphorylating GAPDH at all, but belong to the s ...
... purposes by supplying NADPH for anabolic reactions or in serine biosynthesis (10, 13, 14). Sequence analyses of the genes encoding GAPN of pea and maize, as well as of the bacterium Streptococcus mutans, indicated that GAPN enzymes are not related to phosphorylating GAPDH at all, but belong to the s ...
PDF - Biochemical Society Transactions
... acids. However, since the proposed mechanism of inactivation results in the suicide substrate being bound to the coenzyme rather than the enzyme protein it may be more successful with enzymes that bind pyridoxal phosphate tightly. We thank the M.R.C., S.E.R.C. and Queen's University for support. All ...
... acids. However, since the proposed mechanism of inactivation results in the suicide substrate being bound to the coenzyme rather than the enzyme protein it may be more successful with enzymes that bind pyridoxal phosphate tightly. We thank the M.R.C., S.E.R.C. and Queen's University for support. All ...
PLP-dependent Enzymes: a Powerful Tool for - Beilstein
... called generalist in opposition to specialist enzymes, which evolved to catalyze one reaction on a unique primary substrate. They represent a significant portion of the total enzymes in a living cell and play more than one physiologically important role [15]. Besides this natural eclecticism, many e ...
... called generalist in opposition to specialist enzymes, which evolved to catalyze one reaction on a unique primary substrate. They represent a significant portion of the total enzymes in a living cell and play more than one physiologically important role [15]. Besides this natural eclecticism, many e ...
REVIEW ARTICLE `New uses for an Old Enzyme
... in the second domain (Falzon & Davidson, 1996). Thus rather than having a common binding site for both the reducing and oxidizing substrates (as in OYE), these enzymes would appear to have separate sites, permitting optimization of each site for the half-reaction it catalyses. However, a two-domain ...
... in the second domain (Falzon & Davidson, 1996). Thus rather than having a common binding site for both the reducing and oxidizing substrates (as in OYE), these enzymes would appear to have separate sites, permitting optimization of each site for the half-reaction it catalyses. However, a two-domain ...
AMP-forming acetyl-CoA synthetases in Archaea show
... with the MEGA program (Kumar et al. 1994) using a neighbor joining algorithm with a gamma distance estimation (γ = 2). The phylogeny was constructed based on pairwise distance estimates of the expected number of amino acid replacements per site (0.2 in the scale bar). One thousand bootstrap replicat ...
... with the MEGA program (Kumar et al. 1994) using a neighbor joining algorithm with a gamma distance estimation (γ = 2). The phylogeny was constructed based on pairwise distance estimates of the expected number of amino acid replacements per site (0.2 in the scale bar). One thousand bootstrap replicat ...
Immobilization_Mecha..
... relation to the extension of the charged analyte molecule from the substrate interface.11−13 By directly immobilizing the probe to the gate surface, a larger portion of the charged molecule will reside within the Debye length, which should increase sensitivity of the sensor. Our group has previously ...
... relation to the extension of the charged analyte molecule from the substrate interface.11−13 By directly immobilizing the probe to the gate surface, a larger portion of the charged molecule will reside within the Debye length, which should increase sensitivity of the sensor. Our group has previously ...
structure and mechanism of dna polymerases
... well as the eukaryotic telomerases. During the course of reverse transcription, retroviral RTs interact with a variety of different nucleic acid substrates (RNA/RNA, DNA/RNA, RNA/DNA, DNA/DNA) to convert a single‐stranded viral RNA genome into double‐stranded proviral DNA (Gotte et al., 1999). Some ...
... well as the eukaryotic telomerases. During the course of reverse transcription, retroviral RTs interact with a variety of different nucleic acid substrates (RNA/RNA, DNA/RNA, RNA/DNA, DNA/DNA) to convert a single‐stranded viral RNA genome into double‐stranded proviral DNA (Gotte et al., 1999). Some ...
Prokaryotic features of a nucleus
... located inside the chloroplast. Antisera raised against the chloroplast enzyme do not cross-react with the cytosolic GAPDH and fingerprints as well as amino acid compositions are different for the two enzymes [33 - 351. The chloroplast GAPDH is also exceptional in that it is composed of two separate ...
... located inside the chloroplast. Antisera raised against the chloroplast enzyme do not cross-react with the cytosolic GAPDH and fingerprints as well as amino acid compositions are different for the two enzymes [33 - 351. The chloroplast GAPDH is also exceptional in that it is composed of two separate ...
Hydrogel-Encapsulated Fluorophore-Enzyme Conjugates for Direct
... result in a decrease in the microenvironment pH and an increase in the measured fluorescence intensity. SNAFL-1 is a selfreferencing dye, which reduces problems associated with photobleaching and decreases sensor noise through the use of ratiometric emission measurements. Recently, a bead-based opti ...
... result in a decrease in the microenvironment pH and an increase in the measured fluorescence intensity. SNAFL-1 is a selfreferencing dye, which reduces problems associated with photobleaching and decreases sensor noise through the use of ratiometric emission measurements. Recently, a bead-based opti ...
Minimalist Active-Site Redesign: Teaching Old Enzymes New Tricks
... their high catalytic efficiencies (often at or near diffusion control) are coupled with tight substrate specificities, limiting their usefulness in nonbiological applications. Protein engineering may provide a way to broaden the scope of enzymecatalyzed transformations, enhancing their synthetic uti ...
... their high catalytic efficiencies (often at or near diffusion control) are coupled with tight substrate specificities, limiting their usefulness in nonbiological applications. Protein engineering may provide a way to broaden the scope of enzymecatalyzed transformations, enhancing their synthetic uti ...
Deoxyribonucleic Acid Base Compositions and Nucleotide
... tide distribution, (TI + guanine-plus-cytosine (G+C) frequency curves (6), describe an additional physicochemical quality of the bacterial genome. The DNA nucleotide distributions of some 2,500 bacterial strains were found to be “quite similar within each genus,” but varied considerably between gene ...
... tide distribution, (TI + guanine-plus-cytosine (G+C) frequency curves (6), describe an additional physicochemical quality of the bacterial genome. The DNA nucleotide distributions of some 2,500 bacterial strains were found to be “quite similar within each genus,” but varied considerably between gene ...
Molecular signatures-based prediction of enzyme
... Supplementary Fig. S2 for an illustrative example). An enzyme is classified as promiscuous if it can process dissimilar reactions, i.e. if the enzyme has been annotated with at least a pair of reactions RA and RB with different signatures. Reaction signature-based characterization of enzyme substrat ...
... Supplementary Fig. S2 for an illustrative example). An enzyme is classified as promiscuous if it can process dissimilar reactions, i.e. if the enzyme has been annotated with at least a pair of reactions RA and RB with different signatures. Reaction signature-based characterization of enzyme substrat ...
Purification and characterization of the 1-3
... 1-3 PPD dehydrogenase (EC 1.1.1.202) was purified to homogeneity from Clostridium butyricum E5 grown anaerobically on glycerol in continuous culture. The native enzyme was estimated by gel filtration to have a molecular weight of 384 200 ⫾ 31 100 Da; it is predicted to exist as an octamer or a decam ...
... 1-3 PPD dehydrogenase (EC 1.1.1.202) was purified to homogeneity from Clostridium butyricum E5 grown anaerobically on glycerol in continuous culture. The native enzyme was estimated by gel filtration to have a molecular weight of 384 200 ⫾ 31 100 Da; it is predicted to exist as an octamer or a decam ...
Amino acid frequency distribution at the enzymatic active site
... this study, the whole dataset was divided in three categories namely Viral (VEs; 9), Prokaryotic (pEs; 481) and Eukaryotic (eEs; 348) enzymes based on the source organism. While preparing the database, repetitive CSA entries were excluded. Additionally, depending upon the cellular localization of th ...
... this study, the whole dataset was divided in three categories namely Viral (VEs; 9), Prokaryotic (pEs; 481) and Eukaryotic (eEs; 348) enzymes based on the source organism. While preparing the database, repetitive CSA entries were excluded. Additionally, depending upon the cellular localization of th ...
Chlamydia trachomatis RNA polymerase major sigma subunit
... by restriction endonuclease digestion. Sequence of 0 Subunit Gene-The gene was sequenced after ligation into M13, using the dideoxy sequencing method of Sanger et al. (28) and modified T7 DNA polymerase (Sequenase, United States Biochemical Corp.). The reaction used either fluorescent primers, with ...
... by restriction endonuclease digestion. Sequence of 0 Subunit Gene-The gene was sequenced after ligation into M13, using the dideoxy sequencing method of Sanger et al. (28) and modified T7 DNA polymerase (Sequenase, United States Biochemical Corp.). The reaction used either fluorescent primers, with ...