Download mRNA transcript practice

DNA Template strand
DNA Complement Strand
3’A A T C G G C T A A T C C G A T C A G T5’
5’T T A G C C G A T T A G G C T A G T C A 3’
1. Does it matter which strand is used to make the mRNA transcript?????
2. PRACTICE :
DNA Template strand
3’ A A T C G G C T A A T 5’
DNA Complement Strand
mRNA transcript
DNA Template strand
5’ T A A C G T A C C T C 3’
DNA Complement Strand
mRNA transcript
Follow the following example for number 3:
DNA : G T A C G C G T A T A C C G A C A T T C
mRNA: C A U G C G C A U A U G G C U G U A A G
Codons: AUG-CGC-AUA-UGG-CUG-UAA
Amino Acids: METHIONINE-ARGININE-ISOLEUCINE-TRYPTOPHAN-LEUCINE
3. Using the example above, transcribe the following DNA strand into mRNA and translate that strand into a polypeptide chain, identifying the
codons, and amino acid sequence.
DNA: A T A C G A A A T C G C G A T C G C G G C G A T T C G G
mRNA:
Codon:
Amino Acids:
4. Directions: Fill in the blanks below using the given template strand:
DNA Template:
3’ T A C T G G T T G T A C C C C C A T A G G A C T 5’
Complementary DNA sequence :
Which is the leading strand ? _____________________________________________
Which is the lagging strand ? _____________________________________________
Complementary mRNA sequence :
(mRNA transcript)
Amino Acid Sequence sequence :_______________________________________________
(polypeptide sequence)
How many nucleotides are present on this DNA template? ___________
5. Fill in the blanks below using the give template strand:
DNA Template:
5’ T T T A C T G G T T G T A C C C C C A T A G G A C T A 3’
Complementary DNA sequence :________________________________________________
Which is the leading strand ? __________________________
Which is the lagging strand ? __________________________
Complementary mRNA sequence :_______________________________________________
(mRNA transcript)
Amino acid sequence : _______________________________________________________
(polypeptide sequence)
Number of Amino Acids ? _______
Number of Codons ? ___________
How many nucleotides are present on this DNA template?