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Table S2 A2/CD20-reactive T cells specifically recognize the CD20188-196 peptide Amino acid sequence Protein shading: % pentamer+ cells of CD8+ cells Cell line 1 Cell line 2 References Cell line 3 homology with CD20p SLFLGILSV CD20 LLFLTPMEV CD19 SLFAAISGM 95 % 75 % 0.06% 0.13% 0.12% 0.02% CD20 0.25% 0.09% 0.04% ILFAFVSSV FLT3 0.14% 0.02% 0.01% ELTLGEFLKL Survivin 0.34% 0.11% 1.28% YLISGDSPV CD33 0.14% 0.00% 0.00% Schmitz M., et al. (2000) Cancer Res 60, 4845-4849 Bae J., et al. (2004) Clin Cancer Res 10, 7043-7052 KLSLGLPGL CD19 0.16% 0.06% 0.03% SMFHVTLKI CLL-1 0.14% 0.07% 0.00% FLDCQIHRV CD127 0.17% 0.09% 0.05% FLLGLGLGV CD7 0.14% 0.06% 0.07% LLWAGALAM CD33 0.13% 0.03% 0.02% FLLLTISLL CD52 0.21% 0.12% 0.06% NLVPMVATV CMV pp65 0.3 % 1.37% SLYNTVATL HIV-1 gag p17 0.22% 0.34% 97 % 0.08% Goulder P.J., et al. (1997) J Exp Med 185, 1423-1433 VLQELNVTV Proteinase 3 0.1 % 0.03% 0.02% Molldrem, J.J., et al. (2003) J Clin Invest 111, 639-647. WLSAAPTSL CD33 0.14% 0.03% 0.01% VLWHWLLRT CD19 0.12% 0.00% 0.04% KLGGDLLRA CD18 0.14% 0.06% 0.07% RLYENRASL HA-1 0.01% 0.00% 0.02% SMLDDLRNV CD18 0.12% 0.03% 0.08% LLLPLLLAL CD7 0.13% 0.13% 0.03% ALARGLPGA CD7 0.16% 0.02% 0.02% KLMSPKLYV CD19 0.11% 0.09% 0.06% Bae J., et al. (2005) Clin Cancer Res 11, 1629-1638 LMGTLIAKV HA-1 0.1 % 0.03% 0.04% HLLGPWLLL CD22 0.07% 0.01% 0.01% KMIMNSLSL CD20 0.16% 0.04% 0.04% GLCTLVAML EBV BMLF-1 2.2 % 0.03% 0.03% Steven M.N., et al. (1997) J Exp Med 185, 1605-1617 RMFPNAPYL WT-1 0.06% 0.01% 0.02% Oka, Y., et al. (2000) Immunogenetics 51, 99-107 FVANAIIHL CD52 0.11% 0.03% 0.01% ALAVISFLL CD7 0.17% 0.15% 0.03% ALPAALAVI CD7 0.16% 0.04% 0.02% LLSGFFFFA CD11a 0.12% 0.03% 0.02% ILYPINILI CD11a 0.14% 0.00% 0.04% FMQPLFLVA HA-1 0.3 % 1.8 % 0.32% GLADVAVGA CD11a 0.18% 0.03% 0.55% IMSDSNYVV FLT3 0.15% 0.06% 0.04% YLGYTVTWL CD11a 0.17% 0.00% 0.09% VMNGTDPEL CD2 0.13% 0.09% 0.07% LIQPFMFRL MPO 0.02% 0.00% 0.00% ELAGIGILTV MART-1 0.3 % 2.0 % Valmori D., et al. (1998) J Immunol 160, 1750-8 ELAGIGILTV FLYALALLL EBV LMP-2 0.6 % 0.9 % CLGGLLTMV EBV LMP-2 0.5 % 0.8 % Lautscham G., et al. (2003) J. Virol. 77, 2757-61 Lee S.P., et al. (1993) J Virol 67, 7428-35 cell lines were stained with this peptide-HLA-multimer in separate experiment homology dependent on starting point for comparison (right or left)
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