Download tutorial DNA - UniMAP Portal

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Document related concepts
no text concepts found
Transcript 
ERT 101 Biochemistry
Tutorial – Nucleic acid
1. What structure do DNA nucleotides and RNA nucleotides have in common?
2. Why is DNA replication termed ‘semiconservative’
3. Explain the central dogma in molecular biology
4. On average, RNA polymerase makes one error for every 10,000 nucleotides it incorporates in
RNA. By contras, only one base pair error remains for every ten billion base pairs during DNA
replication. Explain why the accuracy of RNA transcription is not as critical as the accuracy of
DNA replication.
5. What is the difference between a promoter sequence and origin?
6. What is the function of ligase during DNA replication?
7. Discuss the step involved in cloning a gene
8. If molecules of mRNA have the following nucleotide base sequences, what will be the sequence
of amino acids in polypeptides synthesized in eukaryotic ribosomes?
a. AUGGGGAUACGCUACCCC
b. CCGUACAUGCUAAUCCCU
c. CCGAUGUAACCUCGAUCC
d. AUGCGGUCAGCCCCGUGA
9. Give definition about DNA replication, transcription and translation.
10. Beside the fact that it synthesizes RNA, how does RNA polymerase differ in function from
DNA polymerase?
11. Describe three artificial methods of introducing DNA into cells.
Related documents