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Alien Encounters! Purpose: In this activity, you will determine the traits of recovered aliens by analyzing their DNA and determining the amino acid sequences of the resulting small protein fragments. Each fragment is associated with a particular gene and a specific alien characteristic (trait). Procedure: 1. 2. 3. 4. 5. Put the DNA sequence for each gene in the proper location on your data sheet. Transcribe the DNA for each gene into mRNA. Record those sequences. Translate the mRNA into amino acids using the provided mRNA Codon Chart. Record the amino acid sequence for each gene. Use the “Alien Genes” chart to determine the traits that are associated with each of your amino acids sequences and write those traits on the charts. On the back of the Alien Genes chart, draw your alien and answer the following questions: Questions: 1. 2. 3. 4. 5. 6. Did you find any “identical” aliens in your group? Give the tRNA sequences for Gene D. How does a single change in a nitrogen base alter the formation of a resulting protein? If you knew a particular amino acid sequence, could you figure out the DNA for that sequence? What is the difference between transcription and translation? What are the roles of the DNA, the mRNA, the rRNA, and tRNA in protein synthesis? mRNA CODON CHART DATA Tables for Alien Gene Analysis: Alien Number 1 Is your alien hairless or hairy? GENE A DNA CAAGGATAT mRNA Amino Acids Trait Is your alien fat or skinny? GENE B DNA ACCCAACAA mRNA Amino Acids Trait Does your alien have 4 legs or 8 legs? GENE C DNA AGCAGG mRNA Amino Acids Trait What size nose does your alien have? GENE D DNA GTCAGGAAACCC mRNA Amino Acids Trait Does your alien have antennae or not? GENE E DNA TTTAAA mRNA Amino Acids Trait What color skin does your alien have? GENE F DNA GGACGCCGA mRNA Amino Acids Trait How many fingers does your alien have? GENE G DNA GTCCTCCTA mRNA Amino Acids Trait Does your alien have 4 eyes or 8 eyes? GENE I DNA TATCTACGC mRNA Amino Acids Trait Does your alien have a tail? GENE H DNA CGCCCCTAT mRNA Amino Acids Trait ALIEN GENES This table shows the amino acid sequences for the various alien genes and traits. Gene Letter A B C D E F G H I Amino Acid Sequence Val-pro-ileu Tryp-pro-ileu Tryp-val-val Ileu-ileu-ser Ser-ala Ser-ser Pro-ser-phe-gly Gln-ser-phe-gly Lys-phe Lys-leu Pro-ala-ala Pro-ala-asp Pro-ala-val Pro-ala-pro Gln-glut-asp Gln-glut-lys Gly-gly-ileu ala-gly-ileu Ileu-asp-ala Ser-asp-ala Resulting Characteristic Hairless Hairy Fat Skinny 4 legs 8 legs Long nose Short nose No antennae 4 antennae Blue skin Red skin Yellow skin Green skin 10 fingers 12 fingers Tail No tail 4 eyes 8 eyes