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Supplementary table 1. Complete Formulation for RegES Medium Component Fatty acids * Linoleic acid Arachidonic acid Oleic acid Amino acids Glycine L-histidine L-isoleucine L-methionine L-phenylalanine L-proline L-hydroxyproline L-serine L-threonine L-tryptophan L-tyrosine L-valine Vitamins Thiamine Retinol * Antioxidants Glutathione Ascorbic acid Proteins Human serum albumin Insulin Transferrin FGF basic Activin A * Trace elements Trace elements B Trace elements C Selenium Other components NEAA L-glutamine -mercaptoethanol Concentration (mg/l) Manufacturer 1 1 1 Cayman Chemicals Cayman Chemicals Sigma-Aldrich 53 183 615 44 336 600 15 162 425 82 84 454 Sigma-Aldrich Sigma-Aldrich Sigma-Aldrich Sigma-Aldrich Sigma-Aldrich Sigma-Aldrich Sigma-Aldrich Sigma-Aldrich Sigma-Aldrich Sigma-Aldrich Sigma-Aldrich Sigma-Aldrich 9 0.5 Sigma-Aldrich Sigma-Aldrich 1,5 50 Sigma-Aldrich Sigma-Aldrich 10000 10 8 0.008 0.005 Sigma-Aldrich Invitrogen Sigma-Aldrich R&D Systems R&D Systems 1:1000 1:1000 0.00001 Cellgro Cellgro Sigma-Aldrich 1% 2 mM 0.1 mM Cambrex Bio Science Invitrogen Invitrogen Basal medium: KnockOut DMEM (Invitrogen) * Components added after optimization
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