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NAME___________________________
DATE___________________
HOUR___________________
BIOLOGY I - DIHYBRID CROSSES
HEREDITY - 5
INTRODUCTION – Crosses involving two traits can be followed with a Punnett square just like a Monohybrid. It just
requires a larger square. These types of crosses are called Dihybrids. The major difference is that when following one trait, the
parent can produce a maximum of two gametes. With two triats, a dihybrid, the parent could produce as many as four gametes.
For example, look at a plant that is heterozygous for tallness and heterozygous for pod color. Its genotype would be TtGg. It
can produce four different gametes. Any one of these gametes could be passed on: TG, Tg, tG, and tg. Here is an example of a
Dihybrid cross:
TtGg X TtGg
TG
Tg
tG
tg
TG
TTGG
TTGg
TtGG
TtGg
THE RESULTS:
Tg
TTGg
TTgg
TtGg
Ttgg
tG
TtGG
TtGg
ttGG
ttGg
tg
TtGg
Ttgg
ttGg
ttgg
9 TALL GREEN PLANTS
3 TALL YELLOW PLANTS
3 SHORT GREEN PLANTS
1 SHORT YELLOW PLANT
PROBLEMS – Use the table below to solve the following problems. Determine the gametes that will be produced.
Then using a Punnet square, predict the genotypic ratio and phenotypic ratio for the following crosses. Always write down the
genotype of the parents if it is not supplied for you.
TRAIT
Seed coat color
Seed coat shape
Pod color
Height of plant
HOMOZYGOUS DOMINANT
SYMBOL
LOOKS LIKE
BB
Brown
RR
Round
GG
Green
TT
Tall
HETEROZYGOUS
SYMBOL
LOOKS LIKE
Bb
Brown
Rr
Round
Gg
Green
Tt
Tall
HOMOZYGOUS RECESSIVE
SYMBOL
LOOKS LIKE
bb
White
rr
Wrinkled
gg
Yellow
tt
Short
1. Cross these two plants: TTBB X ttbb
What gametes can the first plant produce? _________ _________ _________ _________
What gametes can the second plant produce? _________ _________ _________ _________
What is the genotypic ratio _________________
What is the phenotypic ratio__________________
2. Cross two offspring from the cross in problem number 1.
What gametes can the first plant produce? _________ _________ _________ _________
What gametes can the second plant produce? _________ _________ _________ _________
What is the phenotypic ratio__________________
3. Cross two plants that are heterozygous for both round seed coats and green pod color.
What gametes can the first plant produce? _________ _________ _________ _________
What gametes can the second plant produce? _________ _________ _________ _________
What is the phenotypic ratio__________________
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