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Exam 3 Review Supplemental Instruction Iowa State University Exam covers material in chapters 19.1, 19.2, 20.1, 20.3, 35, 36.1-36.3 1. Short tandem repeating sequences are a) Coding regions of chromosomal DNA. b) Found at specific locations in the genomes of all species. c) Non-coding sequences in bacterial DNA. d) Non-coding regions of chromosomal DNA. e) B and D only. f) All the above. 2. What is a plasmid? Circular DNA that originated in bacteria. Often used as a vector. 3. Which are techniques used to make multiple copies of a single gene? a) PCR b) Gel electrophoresis c) Gene cloning d) Gene therapy e) A and C only f) C and D only 4. Restriction enzymes a) Cut DNA at specific sequences called restriction sites. b) Discovered by Wener Arber. c) Are naturally present in bacteria to guard against viral infection. d) All of the above. 4. Give an example of a sticky end that could be matched up with the following sticky end: ACGTC T A GCAGT Leader: Course: Instructor: Date: Kali Disterhoft BIOL 212 (2) Dr.Kukday 10/27/14 a) a plant seedling will bend away from the light. b) the hormone auxin will accumulate on the side facing the light. c) the side facing the light will grow more slowly than the side away from the light. d) the hormone auxin will slow growth on the side of the seedling to which the hormone accumulates. e) All of the above. 9. What is the likely mechanism by which an enzymelinked receptor might transduce and amplify a signal intracellularly? a) By activating a protein kinase cascade. b) b. By degrading a repressor. c) c. By upregulating the expression of a transporter. 10.Which is correct? a) System > tissue > cell > organ b) Cell > tissue > organ > system c) Organ > tissue > system > cell d) System > organ > tissue > cell 11. What are the four types of animal tissue? a) Epithelial b) Connective c) Muscle d) Nervous 12. Fertilization produces a a) Oocyte b) Zygote c) Embryo d) None of the above 13. Explain cell differentiation. -The process by which cells develop specific characteristics and functions 5. What is a method used to treat human diseases by the introduction of cloned genes into cells? -gene therapy 6. What are the 3 steps of PCR? 1. Denaturation 2. Primer annealing 3. Primer extension 7. Organisms that carry genes that were introduced using molecular techniques are called? a) Transgenics. b) Recombinant DNA. c) Mutants. d) Genetically modified organisms. e) Both B and D. 8.In response to unidirectional light …….. (finish the sentence) 14. Stem cells are a) pluripotent. b) Undifferentiated cells. c) Maintain their population. d) All of the above. 15. Within an organism, what makes one cell type different from another? -They express different phenotypes. 16. Morphogens are a) Molecules that disrupt normal development. b) Molecules that convey positional information. c) Mutagenic agents that cause apoptosis. 1060 Hixson-Lied Student Success Center 515-294-6624 [email protected] http://www.si.iastate.edu d) e) Receptors that allow cells to adhere to the extracellular matrix. Both a and c. 17.Which of the following genes do not play a role in the process whereby segments are formed in the fruit fly embryo? a) Homeotic genes. b) Gap genes. c) Pair-rule genes. d) Segment-polarity genes. e) All of the above. 18. Some genes are on in all types of cells. a) True b) False 19. Name 3 characteristics of model organisms. a) Small b) Short Generation Time c) There is an abundance of information on them 20. All cells of an organism contain the same DNA and the same set of genes. a) True b) False 21. d) e) That it is not required for making ventral structures. None of the above. 23. Gametes are a) Haploid b) Diploid 24. Meristems a) Are undifferentiated cells. b) Have a self-renewing cell population. c) Grow throughout life. d) Occur at tips of shoots and roots. e) All of the above. 25. What are the 3 types of organs in a plant? a) Stems b) Leaves c) Roots 26. Vegetative growth is a) Determinate. b) Indeterminate. c) Increases the size of the shoot and roots system. d) A and C. e) B and C. 27. Describe apical-basal polarity in plants. -Meristems at the roots and shoots grow in opposite directions. 28. Secondary growth in plants a) Produces lateral meristems. b) Includes the protoderm. c) Increases the girth of stems and roots. . The figure shown above illustrates the expression domains of homeotic genes in Arabidopsis. What would the pattern of structures be (Whorl 1 to Whorl 4) for a loss-of-function mutant in B? A) Sepals, Petals, Stamens, Carpels B) Sepals, Sepals, Stamens, Stamens C) Sepals, Sepals, Carpels, Carpels D) Carpels, Stamens, Stamens, Carpels 22.A loss-of-function mutation in a Drosophila melanogaster gene called dorsal causes a pattern defect in the fly embryo. Mutant embryos are completely dorsalized; that is, dorsal structures form where there should be ventral structures. What does this tell us about the normal function of the dorsal+ gene? a) That it is required for producing dorsal structures. b) That it is not required for producing dorsal structures. c) That it is required for making ventral structures. 29.Where would you look to find the gametophyte generation of a flowering plant? a) At the shoot apical meristem. b) At the root apical meristem. c) In seeds. d) In flower parts. e) Flowering plants lack a gametophyte generation. 30.Shoot apical meristems are……(Finish the sentence) a) totipotent stem cells b) pluripotent stem cells c) bipotent stem cells d) determined cells e) All of the above f) None of the above 31. Expansins do what in plants? -They unzip cross-linking cell-wall polysaccharides so the cell can stretch. 32. Which is not true about Bicoid: a) is a morphogen that regulates Drosophila embryo polarity b) the gene is transcribed in nurse cells and transported into anterior end of Oocyte c) d) is a transcription factor Mutation of its genes leads to embryos with two heads 33. 38. Thigmotropism in plants a) Is a response to light. b) Is a response to gravity. c) Is a response to touch. d) Is a response to rain. 39. Cytokinins.. The figure above illustrates the activation and inactivation of a G protein-coupled receptor. If a nonhydrolyzable form of GTP, a form that does not loose the terminal phosphate group to form GDP, was added to cells, which of the following would be true? a) Help fruit ripening. b) Promote cell division. c) Promote seed germination. d) All the above. 40. What are the 3 types of cell surface receptors? a) G-protein linked A)The ligand would not bind to the receptor. b) Enzyme linked B)The αβγ subunits of the G protein would not dissociate. c) Ligand linked C)Once activated, signaling through the receptor would continue for a longer time than normal. D)None of the above 41. Can large, hydrophilic ligands enter the cell? a) Yes b) No E)All of the above 34. What is the difference between estrogen-mediated response and glucocorticoid meditated response? -estrogen-mediated response receptors are found on the nuclear membrane. Glucocorticoid-mediated responses receptors are in the cytoplasm. 35. What is the role of caspses in the cell? -Caspses breakdown cellular components after apoptosis 36. What are the 3 stages of plant signal transduction? a) Receptor activation b) Signal transduction c) Response 37. The major signaling molecule in plants is a) Abscisc acid b) Auxin c) Ethylene d) Gibberellic acid 42. What is the importance of cAMP? cAMP is a secondary messenger that helps in making a response signal quick and fast.