Download Organic Molecules

Name:_____________________________
Date:______________________________
Semester:____________
Banker
Lab 9: Organic Molecules
Practical 1
Reading: Chapter 2: Chemical Constituents of cells
Figures: 2.12, 2.14, 2.17, 2.19
Define:
Carbohydrate
Monosaccharide
Polysaccharides
Lipid
Triglyceride
Protein
Amino Acid
Iodine
Sudan IV
Biuret Reagent
Grease spot
Peptide Bond
Peptide
Polypeptide
Identify on a Diagram/Model:
Carbohydrate
Triglyceride
Monosaccharide
Protein
Lab:
Iodine Test For Starch
1. Label six test tubes: 1W, 1E, 1G, 1D, 1S, and 1P
2. Add to each test tube 2ml of the following sample liquids:
a. 1W- 2ml distilled water
b. 1E- 2ml egg albumen
c. 1G- 2ml 10% glucose solution
d. 1D- 2ml carbonated soft drink
e. 1S- 2ml 10% starch solution
f.
1P- 2ml potato juice
3. Add 0.5ml iodine solution (IKI).
4. Swirl the tube GENTLY to mix.
5. Note the final color and record below.
6. Wash out and re-use the tubes for the next part of the experiment.
Brown Paper Test for Lipids
1. Separate two areas on a piece of brown paper.
2. Label them “Water” and “Oil.”
3. Give 10 min. for the spots to dry.
4. Record which spots leave a stain (grease spot) and which do not.
Sudan IV Test for Lipids
1. In a test tube add 2ml of water and 2ml of oil.
2. Shake the tube, then let it sit for several minutes.
3. Observe and record the results.
4. Add a few drops of Sudan IV to the test tube.
5. Swirl GENTLY and let sit for several minutes.
6. Record your results.
Biuret Test for Proteins
1. Label six test tubes: 2W, 2E, 2G, 2D, 2S, and 2P
2. Add to each test tube 2ml of the following sample liquids:
a. 2W- 2ml distilled water
b. 2E- 2ml egg albumen
c. 2G- 2ml 10% glucose solution
d. 2D- 2ml carbonated soft drink
e. 2S- 2ml 10% starch solution
f.
2P- 2ml potato juice
3. Add 2ml of Biuret Reagent to each.
4. Swirl GENTLY to mix.
5. Note the final color and record below.
6. Wash out the test tubes and bring them up to the front of the lab.
Lipid
Amino Acid
Results:
Iodine Test For Starch
Tube
Contents
1W
Distiller Water
1E
Egg Albumin
1G
10% Glucose Solution
1D
Soft Drink
1S
10% Starch Solution
1P
Potato juice
Color
Starch Present or Absent
Brown Paper Test for Lipids
What did you observe after giving
both spots on the brown paper time
to dry?
Sudan IV Test for Lipids
What did you observe after giving
shaking the test tube containing
water and oil?
Substance
Oil
Color without Sudan IV
Color with Sudan IV
Observations: Lipids Present or Absent
Water
Biuret Test for Proteins
Tube
Contents
2W
Distiller Water
2E
Egg Albumin
2G
10% Glucose Solution
2D
Soft Drink
2S
10% Starch Solution
2P
Potato juice
Color
Protein Present or Absent