Download LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY
SECOND SEMESTER – APRIL 2006
IB 37
BT 2952 - MEDICAL BIO-TECHNOLOGY
Date & Time : 26-04-2006/9.00-12.00
Dept. No.
Max. : 100 Marks
PART – A
Answer all the Questions
I
(20 marks)
Choose the best answer
(51=5)
1. FACS refers to
(a) Fine activated Cell Separator
(b) Fluorescence Activated Cell Sorter
(c) Fluorescence Absorbent Culture Sorter
(d) Fluorescent Activated Culture Cell.
2. Which one of the following is a DNA oncogenic virus?
(a) SV40 virus (b) HIV
(c) Rous sarcoma virus (d) Reovirus
3. Phosphoramidite linkage in antisense oligonucleotides makes them
(a) Unstable (b) Resistant to nucleases (c) Shorter (d) Bind better with mRNA
4. Which one of the following is useful for PCR-based tissue typing?
(a) ISO probes (b)MLR
(c)SSO probes (d)Trypan blue
5. Recombination fraction between two specific gene loci
(A)
Σ (R) ____
Σ (R) + Σ (NR)
(B) i.e, it is the ratio of the recombinants to the total offspring.
(a) Only statement A is true
(b) Only statement B is true
(c) Both Statement A and B are true
(d) Both Statement A and B are false
II
State whether true or false, if false give reasons
(51=5)
6. The 5’ and 3’ ends of mRNA are good target sites for antisense oligonucleotides.
7. Diagnostic probes for pathogens are identified from a genomic library of that
pathogen.
8. Trypsinization is used to cleave cell surface proteins from cells in culture.
9.
Nuclear transfer is used in embryo cloning .
10. The shot-gun method is a top-down approach of gene sequencing.
III Complete the following
(51=5)
11. Stem cells that differentiate into endothelial cells are identified by _________cell
surface markers.
12. Genetically manipulated animals are said to be _______
_________.
13. __________ are made up of short oligonucleotides synthesized and attached to
quartz wafers.
14. __________ gene therapy involves correction or insertion of remedial gene
outside the patient’s body.
15. _____________ gene cloning entails mapping a disease gene of unknown function to
a chromosome location.
IV Answer in 50 words
(51=5)
16. Expand RT/QPCR. Mention its applications.
17. What is hybridoma technology.
18. What are the types of adult stem cells?
19. Mention the characteristics of chimeric antisense oligonucleotides.
20. EST’s
PART- B
Answer any five questions, each within 350 words.
(58=40)
21. Write about transgenic pigs.
22. Give an account on recombinant vaccines.
23. Write notes on Embryonic stem cells and Embryonic germ cells.
24. Write a note on PCR-based tissue typing.
25. Describe RT/QPCR as a tool in study and diagnosis of diseases.
26. Discuss the use of antisense oligonucleotides in disease therapy.
27. Explain the mapping, which is used to study the possible candidate for disease
genes.
28. Explain the HGP and, the goals and events concerning the project.
PART – C
Answer the following each within 1500 words.
(220=40)
29. a) Give an account of disease resistant transgenic birds and rabbits.
(OR)
b) Write notes on:
i. Conditions for animal cell culture. (12)
ii. Oncogenic viruses
(8)
30. a) Write notes on
i. Pharmacogenomics
(10)
ii.Oligonucleotide correction of genetic conditions (10)
(OR)
b). Explain:
i. DNA Microarrays
(10)
ii. Sanger’s method of gene sequencing. (10)
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