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UNIVERSIDAD DE BARCELONA
Facultad de Farmacia
Departamento de Bioquímica y Biología Molecular
REDESIGN OF CARNITINE ACETYLTRANSFERASE
SPECIFICITY BY PROTEIN ENGINEERING
ANTONIO FELIPE GARCIA CORDENTE
2006
OBJECTIVES
Objectives
The objectives of the present thesis are
1. Identification of the amino acids responsible for the different acyl-CoA chainlength specificity of the members of the carnitine acyltransferase family.
2. Identification of the amino acids involved in choline-carnitine discrimination in
the carnitine acyltransferase family of proteins.
3. Analysis of the effect of overexpressing CrAT on ester production during
alcoholic fermentation in yeast and its possible biotechnological applications.
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