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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – FOOD CHEMISTRY AND FOOD PROCESSING
SECOND SEMESTER – APRIL 2014
FP 2807 - FOOD BIOCHEMISTRY
Date : 01/04/2014
Time : 09:00-12:00
Answer ALL the questions.
Dept. No.
Max. : 100 Marks
Part A
10x2=20 marks
1.
2.
3.
4.
5.
What is the role of Fe-S centres in Electron Transport Chain?
Which site of the Electron Transport Chain is inhibited by rotenone?
Glucose6 phosphate + 2 NADP+ + H20-------------->
Mention the rate limiting steps involved in glycolysis
List the enzymes used for unwinding the double helical structure of DNA. What is the role of DNA
gyrase in replication?
6. Mention the purpose of adding SDS in determining the molecular weight of proteins.
7. Name the enzymes present in the fatty acid synthase complex.
8. What is phenylketonuria? Give the sequence of reaction highlighting the zone of blockage.
9. What is the role of the carnitine shuttle in fatty acid metabolism?
10. What is insulin resistance? Illustrate with a diagram.
Part B
Answer any EIGHT questions.
8x5=40 marks
11. Explain the reactions involved in complex II of Electron transport chain.Why is complex II not
directly involved in energy generation?
12. Describe the transamination reactions .Enumerate its significance in protein metabolism.
13. Why is Ammonia considered as a toxic metabolite? Explain the possible route of its excretion.
14. Explain the role of various enzymes involved in DNA replication.
15. Explain the various types of solute transport systems functioning in biological membranes.
16. Write a note on preparative phase of glycolysis.
17. Enumerate F0 F1 ATP synthase complex.
18. What are the factors that regulate the synthesis of cholesterol?
19. Give the energetics of Palmitic acid oxidation.
20. Explain the oxidation of odd carbon chain fatty acids in our system.
21. Mention the various disorders that are caused due to insulin resistance.
22. Briefly explain the stages of Cholesterol synthesis.
Answer any FOUR questions.
Part C
4x 10=40 marks
23. Explain the major route of energy generation through carbohydrate metabolism.
Calculate its energetics.
24. Write a detailed note on invivo protein synthesis.
25. Explain the pathway which yields riboses and NADPH from Glucose.
26. Discuss the beta oxidative pathway of fatty acids giving the energetics for stearic acid.
27. Discuss any two glycogen storage diseases.
28. Explain the hormonal regulation of water balance in our system.
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