Download LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY
SECOND SEMESTER – APRIL 2010
BT 2954 - MICROBIAL & BIOPROCESS TECHNOLOGY
Date & Time: 26/04/2010 / 1:00 - 4:00
Dept. No.
Max. : 100 Marks
PART A
(20 marks)
Answer All questions
I Choose the Correct Answer
( 5 x 1 = 5 marks)
1. Bioprocess is infinitely easier to improve the productivity is greater in
a. Bacteria
b. Plant cells
c. Animal cells
d. Actinomycetes
2. Which one of the following is not an antifoam agent?
a. Silicones
b. Fatty acids
c. Alcohols
d. Caesein
3. Common problem in bacterial fermentation is due to
b. Fungus
b. Other bacteria
c. Bacteriophages
d. Actinomycetes
4. ------------- is least polar solvent used in liquid – liquid extraction of fermented products.
a. Hexane
b. Benzene
c. Diethyl ether
d. Ethyl acetate
5. Find out the odd one used in effluent treatment.
a. Fluidized filters
c. Rotating biological contactors
b. Trickling filters
d. Upflow anaerobic sludge blankets
II State whether the following statements are True or False. If false give reason.(5 x 1 = 5 marks)
6. Auxotrophs requires minimal medium for the growth.
7. Antifoam agents are spread throughout the medium.
8. Online sensor is an integral part used directly for control the bioprocess.
9. Cyanides are used as precursor for Vitamine B12 production.
10. Gluconobacter is used for acetic acid production.
III. Complete the following
( 5 x 1 = 5 marks)
11. Fermented product of pharmaceutical importance should be inspected and approved by ----------------------- of FDA.
12. Key enzymes for alcohol production are -------------- and --------------.
13. ------------------ method is a pilot scale experiment in lab to mimic industrial conditions.
14. Freezing and drying the cells under vacuum is known as -----------------.
15. The first recombinant protein licensed for therapeutic use is ---------------.
IV. Answer all the questions, each in about 50 words
Write notes on:
16. HEPA
17. Scale-up process
18. Biosensors
19. Rate zonal centrifugation
20. Hops
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( 5 x 1 = 5 marks)
PART B
( 5 x 8 =40 marks)
Answer any FIVE of the following, each within 350 words only. Draw diagrams and
flowcharts wherever necessary.
21. Describe the methods of sterilization for fermentors.
22. Enumerate and explain the environmental conditions required for industrial microbes.
23. How are industrial important microbes are preserved?
24. Write notes on upstream processing.
25. Comment on “Online control of Bioprocess”.
26. Give the outline for the purification of charged molecules.
27. Write details on cheese production.
28. Bring out the production procedure for beer and wine.
PART C
(2x 20 = 40 marks)
Answer the following, each within 1500 words only. Draw diagrams and flowcharts wherever
necessary.
29. (a) Explain various strategies of strain improvement for industrial microbes.
Or
(b) With illustrations, explain different types of bioreactors.
30. (a) Describe the roles of chromatographic techniques in enzyme recovery in bioindustries.
Or
(b) Give the procedure for mushroom production in large scale.
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