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AP BIOLOGY
Unit 8 Ch. 18, 19, 20
“biotechnology”
THINKING AHEAD:
 Lab Materials: Electrophoresis chambers, stain, buffer, micropipettes, tips.
 Teacher Print Work:  lab  lab quiz  test  essay
 Student Print Work:  assign.sheet,  obj. sheet  study sheet,  essay prep
Assignments are given for Campbell Biology 7e. Please use your old text.
Biology in Focus, 1e: Ch.17 Viruses, Ch.24 Bacteria, Ch.13.4 Biotech.
CHAPTER eighteen:
“The genetics of Viruses and Bacteria“
day 1: DUE: Ch. 18 obj. 5-10, 13, 24, 30, 32, 34.
Lecture: Lytic and lysogenic cycles, viruses and life, viral infections, viroids and prions, operons.
CHAPTER 26, 27:
“Bacteria “
day 2: Lecture: Bacteria. Review of bacteria and early Earth.
Lab set-up
CHAPTER twenty:
“DNA Technology and Genomics“
day 3: DUE: Ch.20 obj.1-14.
Lecture: blunt & sticky ends, cloning plasmids, ID recombinant cells, PCR, Southern Blot, RFLP.
day 4:
DUE: Ch.20 obj. 15-20, 24-30
Lecture: review DNA Technology, Human Genome Project, SNP’s and evolution, practical applications.
day 5:
DUE: Read lab “Background,” do “Analysis” #3-14
Discuss Lab background, do #14 analysis.
Pre-lab: “DNA Fingerprinting” LabBench, Lab#6b.
Lab: Pour agarose gels, set up electrophoresis chambers, and practice pipetting.
day 6:
Lab: “DNA Fingerprinting”
Once gels are running, discuss Analysis #14
day 7:
Lab: read gels (complete data tables 1-4 and analysis #1,2), photograph, correct/turn in.
Study for lab quiz.
day 8:
Lab Quiz
Ch.18-20 Test 30 m.c. (10/chapter), essay*
*Essay:
The human genome illustrates both continuity and change.
a. Describe the essential features of the procedures/techniques below. For each, explain how its
application contributes to understanding genetics.

The use of a bacterial plasmid to clone and sequence a human gene

Polymerase chain reaction (PCR)

Restriction fragment length polymorphism (RFLP) analysis.
b.
All humans are nearly identical genetically in coding sequences and have many proteins that are identical
in structure and function. Nevertheless, each human has a unique DNA fingerprint. Explain this apparent
contradiction.
Mrs. Loyd
Page 1 of 1
Campbell Biology 7/E
5/25/2017
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