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PGDAB SYLLABUS
SEMESTER I
PAPER-I : BIOCHEMISTRY I
Credit I : Chemistry of carbohydrates, lipids amino acids and nucleic acids.
Credit II : Metabolism of carbohydrates,
Credit III: Metabolism of amino acids and lipids
Credit IV: Metabolism of nucleotides and nucleic acids.
1. Zubay, G. (1998) Biochemsitry, 4th Edition, Wm C. Brown Publ.
2. Lehninger, A. L., Nelson, D. L. and Cox, M. M. (1997) Principles of Biochemistry.
3. Voet, D. and Voet, I. G. (1995) Biochemistry, 2nd Edition, John Wiley & Sons..
PAPER-II : BIOCHEMISTRY II
Credit I: Cell structure and function- Comparative aspects of bacterial, plant and animal cells.
Credit II: Biomembranes and transport systems and Nutritional Biochemistry
Credit III : Enzyme catalysis, cofactors, coenzymes, enzyme kinetics
Credit IV: Enzyme regulation. Electron transport chain and theories of energy coupling
Books :
1. Zubay, G. (1998) Biochemsitry, 4th Edition, Wm C. Brown Publ.
2. Lehninger, A. L., Nelson, D. L. and Cox, M. M. (1997) Principles of Biochemistry.
3. Voet, D. and Voet, I. G. (1995) Biochemistry, 2nd Edition, John Wiley & Sons..
PAPER-III: ANALYTICAL BIOCHEMISTRY
Credit I: Biological buffers – amino acids and proteins; Molecular interactions; Ion specific
electrodes;
Credit II: Microscopy, spectroscopy and radioactivity.
Credit III: Principles, techniques and applications of, chromatography (partition, gel
permeation, ion exchange, affinity, GC, HPLC, and FPLC)
Credit IV: Electrophoresis and centrifugation: Principles of protein purification and enzyme
assays.
Books :
1. Biophysical Chemistry Upadhyay, A. 2nd ed 1992.
2. Biologists guide to principles and techniques of Practical Biochemistry Wilson, K. and
Goulding, K. H. 3rd ed. 1986.
3. Physical Biochemistry by Friefelder 4th ed 1989.
SEMESTER II
PAPER I : MOLECULAR BIOLOGY & RECOMBINANT DNA TECHNOLOGY
Credit I : Genome organisation in prokaryotes and eukaryotes replication in prokaryotes;
Credit II: Transcription, Translation and their regulation in prokaryotes, post translational
modifications of proteins.
Credit III : Properties of nucleic acids; properties and applications of enzymes used in
recombinant DNA methods; vectors and their hosts.
Credit IV: Principles and uses of nucleic acid hybridizations. Principles and applications of
Polymerase chain reaction. Principles and methods of nucleic acid sequencing.
Books:
1. Genes VII: Lewin B 2000
2. Molecular cloning A Laboratory manual, Sambrook Joseph & Russell David W. , CSHL N.Y,
Ed. 3rd 2001, Vol. 1,2 & 3
3. Principles of Gene manipulation, Primrose Sandy, Twyman Richard & Old Bob, Blackwell
Science, Ed. 6th 2001.
PAPER II: PHYSIOLOGY
Credit I : Study of structure and function of gastrointestinal tract, excretory system in normal
and pathological conditions.
Credit II : Cardiovascular system and Blood and respiratory system
Credit III: Endocrine system in normal and pathological conditions.
Credit IV: Microbiological Asays, Toxicity testing. Strain improvement
Books:
1. Textbook of medical physiology Guyton A C and Hall J E Harcourt Asia Pvt. Ltd. Ed 10 th
2001.
2. Review of medical physiology Ganong W F Appleton and Lange Ed 19th 1999.
PAPER III : IMMUNOLOGY and MICROBIOLOGY
Credit I : Nature of immune responses and Immune system, structure and properties of antibodies,
complement system and immunodiagnostics,
Credit II: Allergy, hypersensitivity, autoimmune disorders and vaccines.
Credit III: Microbiology: Pathogenic organism such as viruses, bacteria.
Credit IV: Use of Microorganisms for industrial applications, antibiotics, vitamins, industrial
enzymes.
Books :
1. Immunology, Kuby J et al Freeman & Co. N.Y. ed 5th 2003.
2. Medical Microbiology R. Cruickshank 4th ed. 1987.
3. Fermentation microbiology and biotechnology, Ed. Mansi EL Mansi & Charlie Bryce, Taylor
& Francis group London , 2002.
4. Basic Biotechnology, Ed. Colin Ratledge Bjorn Kristiansen, Cambridge Univ. Press, Ed. 2nd,
2001.
PRACTICALS
SEMESTER I
I. General Orientation : Weights, Measures, Standards, Beer-Lambert's law, Buffers.
with MSc (P) BC+ MBT
2wk
II. Biochemical Analysis : DNA/RNA Estimation, P, Fe, Ca, Riboflavin/NADH, Vitamin C
estimations. .
2wk with MSc (P) BC+ MBT
III. Physiology: Blood collection, Hematocrit determintions, Cell counting, Glucose estimations,
Cholesterol and lipid profile, Kidney and liver function tests. 6wk with MBT(P)
IV. Enzymology : Protein estimation methods, Enzymes activity estimation, Enzyme Kinetics,
3wk -alone
SEMESTER II
I. Microbiology : Isolation Techniques, Morphology Studies, Milk Grading, Oligodynamic action
of heavy metals, Media Preparation, motility test, UV radiation, BOD and COD. 3 wks
II. Molecular Biology : Isolation of Genomic DNA, Plasmid Preparation and Electroelution. 3
wks
III. Immunology : Ouchterlony, ELISA and Antibody Purification. 1 wk with MBT(P)
IV. Animal Experiments : Breeding, Techniques like Injecting, blood collection, tube feeding and
dissection. 1wk with MBT(P)
V. Internship in any industry /research laboratory for one month. 4 wks