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Biol 211
Lab 8
PreLab Discussion Part I
1. In this lab we will transform bacteria with a plasmid
http://en.wikipedia.org/wiki/Plasmid
http://en.wikipedia.org/wiki/Transformation_(genetics)
2. How is a genetic transformation different than a cancerous transformation?
3. What are the three genes of interest on our plasmid? What is the purpose of each?
4. We have 4 experimental plates. What are your expected results in terms of growth and green color
under the fluorescent light.
+ pGLO on LB/amp
+ pGLO on LB/amp/ara
- pGLO on LB/amp
- pGLO on LB
5. Sterile Technique
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