Download Study Guide 3 - Microscopy Chpt. 3

Independent Study Guide - Microscopy and Cell Structure (Chapter 3)
I.
Microscopy (section 3.1)
a. Light microscopes - maximum magnification ∼1000X
i. contrast/resolution
ii. wet mounts
iii. stained specimens
b. Electron microscopes - maximum magnification ∼ 100,000X
II.
Microscopic techniques: dyes and staining (section 3.2; table 3.2)
a. Simple stains
b. Differential stains
i. Gram stain - separates bacteria into categories based on type of cell wall
1. Gram-positive – purple
2. Gram-negative – pink
c. Special stains - detect specific structures (capsules, endospores, flagella)
d. Fluorescent dyes and tags
III.
Morphology of prokaryotic cells (section 3.3)
a. Shapes (figure 3.20)
i. cocci
ii. rods
iii. coccobacillus
iv. vibrio
v. spirillum
vi. spirochete
b. Groupings (figure 3.22)
i. chains
ii. packets
iii. clusters
IV.
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Study Questions
What is an advantage of a wet mount over staining, and vice versa?
What is an advantage of an electron microscope over a light microscope, and vice versa?
How is a differential stain different from a simple stain?
What does a Gram-positive cell look like? How about a Gram-negative cell?
Describe the shape of a coccus versus a rod.
Structure/function (day 1) -
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