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Transcript 
Purification Enzyme
Aulanni’am
Biochemistry Laboratory
Chemsitry Depatment
Brawijaya University
Aulani "Biokimia Enzim"
Presentasi 8
How to Separate These Objects
Shape
Size
Density
1
1
2 3 4 5 6
10 11
12
wood stone cotton wood wood cotton stone wood stone cotton stone cotton
2 3
Shape
6
Size
4 5 6
4
7 8
Density
5
7
9
7 8 9
10 11
12
Sieving different sizes
cotton
8
4
8
wood
stone
5
Different sedimentation
Aulani "Biokimia Enzim"
Presentasi 8
Different rolling speed
Basic Principles of Protein Purification
Organelle
Cell
Homogenization
Small molecule
Amino acid, Sugar,
Nucleotides, etc
Macromolecule
Nucleic
acid
Protein
Ammonium sulfate
Size
Charge
Carbohydrate
(Lipid)
Cell
Debris
fractionation
Polarity
Affinity
Reverse phase
Ion exchange,
Gel filtration,
Affinity
Chromatofocusing, chromatography, chromatography,
SDS-PAGE,
Disc-PAGE,
HIC,
Ultrafiltration Isoelectric focusing
Hydroxyapatite
Salting-out
Aulani "Biokimia Enzim"
Presentasi 8
Aulani "Biokimia Enzim"
Presentasi 8
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