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All living things are made of cells. Cells are the basic unit of structure and function in living things. New cells are produced from existing cells. Anton van Leeuwenhoek Dutch Businessman 1600’s Invented first powerful microscope (300X) Examined pond water Saw “little animals” Simple Microscope-1 lens. Ex. Magnifying lens Compound Light Microscope- uses light and many lenses to view small objects. Ex. *Our Lab* 1.) Body Tube- tube of mirrors connecting objective lens and eyepiece 2.) Revolving Nosepiece- moves lens into place 3.) Low Power Objective- 4x or 10x; locates specimen 4.) Medium Power Objective- 10x; locates specimen 5.) High Power Objective- 40x; greater magnification 6.) Stage Clips- holds slides in place 7.) Diaphragm- controls the amount of light 8.) Illuminator/Light 9.) Eyepiece (Ocular Lens)- piece you look through, magnifies 10 times (10x) 10.) Arm- supports body tube 11.) Stage- holds the slide Stage Opening- circle where light passes through 12.) Coarse Adjustment- used for FIRST focusing, moves body tube 13.) Fine Adjustment- sharpens image, final focusing 14.) Base- bottom Slide- holds the specimen (glass or plastic) Slide Cover- covers specimen so it doesn’t move Lens Paper- cleans lenses One hand on arm, one hand on base, electric cord securely wrapped and looking forward. Ocular Lens X Objective Lens =Total Magnification 10X 10X 10X X X X 4X 10X 40X = = = 40X 100X 400X Put slide under stage clips. Focus using low power (4X). Coarse -> Fine Switch to higher power (10X or 40X). *ONLY USE FINE ADJUSTMENT ON 40X LENS! Robert Hooke- English Physicist observed plant cells named the word “cell” (Jail cells) Cell Membrane- ALL cells are surrounded by a thin flexible barrier. Nucleus- Large membrane enclosed structure that contains genetic material in the form of DNA and controls many of the cells activities. Enclose DNA in their nuclei Cells that do not enclose DNA in their nuclei Prokaryotic Eukaryotic Simple Complex Unicellular Mostly Multicellular No Nucleus Nucleus No Organelles Organelles First Cells on Earth Ex. Bacteria Ex. Plant & Animal Cells Plant Cell Animal Cell Eukaryotic Eukaryotic Cell Wall Cell Membrane Photosynthesis/Chlorophyll Obtain Energy/ Eat Boxlike Shape Various Shapes